Transgenic carrier system for promoting cell transplantation and gene expression and application thereof

A transgenic vector, gene expression technology, applied in the direction of plant gene improvement, application, vector, etc., can solve problems such as hindering application, uncommon target gene characteristics, tumor occurrence, etc., to avoid the influence of drug toxicity and achieve the effect of great application value
CN110373428AInactive Publication Date: 2019-10-25HANGZHOU DIANZI UNIV
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Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Applications(China)
Current Assignee / Owner
HANGZHOU DIANZI UNIV
Publication Date
2019-10-25
Estimated Expiration
Not applicable Β· inactive patent

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Abstract

The invention discloses a transgenic carrier system for promoting cell transplantation and gene expression and application thereof. The system comprises a gene selecting system, a target gene expression system and a carrier used for carrying the two systems, wherein the gene selecting system is a system for silencing genes for inhibiting division or promoting cell death. Compared with the prior art, the system is not limited by whether the targeted gene has growth advantages or not; selecting is generated in the stem cell level and the differentiated cell level, and therefore, the quantity ofgenetically engineered cells can be stably kept or increased in the whole lifetime; selecting is conducted without drugs, and the influence of the drug toxicity is avoided; moreover, a mutant which only inhibits division or promotes cell death is employed, so that there is no risks of causing cancer.
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Description

[0001] Original application date: 2016-10-08, original application number: 201610877184.9

[0002] Original patent title: A transgenic carrier system and its application for promoting cell transplantation and gene expression technical field

[0003] The invention relates to a transgene carrier system, especially a carrier system capable of promoting cell transplantation and gene expression. Background technique

[0004] Gene therapy or the establishment of adult (stem) cell-based animal bioreactors or animal models require stable target gene expression levels and stable target gene expression positive cell numbers in recipient patients or animals. However, in reality, it generally occurs that the efficiency of expressing the target gene in a single cell decreases and the proportion of cells expressing the target gene decreases. An approach to address the above deficiencies is to perform in vivo screening. There are currently three types of in vivo screening methods: 1) The...

Claims

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