Preparation method, application and detection method of molecular imprinting nanofiber reinforced basement membrane

A nanofiber membrane and nanofiber technology, applied in the field of analysis, can solve the problems of easy aggregation and sedimentation of metal nanoparticles, lower utilization rate of metal particles, and not easy to disperse, etc., achieve good SERS effect, simplify preparation steps, and improve SERS detection effect Effect

Active Publication Date: 2019-10-25
SOUTHEAST UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the metal nanoparticles directly used in this method are easy to coagulate, not easy to disperse in the high-viscosity electrospinning solution, and easy to oxidize during the spinning process. In addition, there are more metal particles concentrated inside the fiber, and the metal Reduced particle utilization

Method used

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  • Preparation method, application and detection method of molecular imprinting nanofiber reinforced basement membrane
  • Preparation method, application and detection method of molecular imprinting nanofiber reinforced basement membrane
  • Preparation method, application and detection method of molecular imprinting nanofiber reinforced basement membrane

Examples

Experimental program
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Effect test

Embodiment 1

[0040] Example 1: Preparation of silver-molecularly imprinted nanofiber reinforced basement membrane

[0041] Weigh 2.5g of polyethersulfone resin and dissolve in 10mL of N,N-dimethylformamide (DMF), stir at 60℃ until it is completely dissolved, add 0.25g of chlorpyrifos (template molecule), stir at room temperature for 12h, then add 0.3g Silver-molecularly imprinted nanofiber electrospinning solution can be obtained by using silver nitrate and sonicating at 40°C for 1 hour.

[0042] Put the configured electrospinning solution into the glass syringe and fix it on the micro syringe pump, choose aluminum foil paper to fix it on the receiving plate, and connect the positive and negative electrodes of the high voltage power supply to the needle of the glass syringe and the receiving plate. Spinning conditions were set as follows: positive voltage 15kV, negative voltage 3.8kV, receiving distance 27cm, spinning solution flow rate of 0.8mL / h, temperature of 25°C, humidity of 60-70%. Put ...

Embodiment 2

[0043] Example 2: Preparation of gold-molecularly imprinted nanofiber reinforced basement membrane

[0044] Weigh 3g of polysulfone (PSF) resin and dissolve in 10mL of N,N-dimethylformamide (DMF), stir at 60℃ until it is completely dissolved, add 0.3g of chlorpyrifos (template molecule), stir at room temperature for 12h, then add 0.25 g gold chloroauric acid, 40 ℃ constant temperature ultrasound for 1 hour to obtain gold-molecularly imprinted nanofiber electrospinning solution.

[0045] Put the configured electrospinning solution into the glass syringe and fix it on the micro-syringe pump, choose aluminum foil paper to fix it on the receiving plate, and connect the positive and negative electrodes of the high-voltage power supply to the needle of the glass syringe and the receiving plate. Spinning conditions are set as follows: positive voltage 14.5kV, negative voltage 3.5kV, receiving distance 25cm, spinning solution flow rate is 0.9mL / h, temperature is 25°C, humidity is 60-70%. ...

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Abstract

The invention discloses a preparation method, an application and a detection method of a molecular imprinting nanofiber reinforced basement membrane. The preparation method comprises the steps of: (1)weighing a functional polymer, dissolving the functional polymer in an organic solvent, adding template molecules into the solutino, stirring and mixing the mixture, adding a nano metal particle precursor into the mixture, and performing ultrasonic dispersion on the mixture to obtain a metal-molecular imprinting nanofiber electrostatic spinning liquid; (2) subjecting the prepared electrostatic spinning liquid to electrostatic spinning to obtain a nanofiber membrane; (3) putting the nanofiber membrane obtained in the step (2) into a reducing agent for reduction; (4) and eluting the template molecules in the reduced nanofiber membrane to obtain the molecular imprinting nanofiber reinforced basement membrane. The preparation method applies the molecular imprinting technique and the nanofibermaterial to the surface-enhanced Raman spectroscopy, realizes the specific identification of the basement membrane and improves the adsorption capacity of the basement membrane, thereby improving thedetection sensitivity.

Description

Technical field [0001] The invention belongs to the field of analysis technology, and particularly relates to a preparation method, application and detection method of a molecularly imprinted nanofiber reinforced basement membrane. Background technique [0002] Pesticide residue detection based on surface-enhanced Raman spectroscopy (SERS) technology has attracted wide attention because of its simple and fast operation, detection of low-concentration substances, portable and convenient detection instruments, and real-time detection. One of the most dynamic methods in pesticide residue testing. However, because the general enhanced substrate does not have selectivity, the interference of impurities cannot be eliminated in the detection process, and the actual detection effect is often not ideal, which restricts the further development of SERS technology. [0003] Molecular imprinting technology refers to simulating biological recognition systems such as antigen-antibody, enzyme, et...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65G01N1/34D01F6/94D01F1/10
CPCG01N21/658G01N1/34D01F6/94D01F1/10D01D5/003D01D5/0092D04H3/033
Inventor 戎非邓剑军付德刚吴巍
Owner SOUTHEAST UNIV
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