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Preparation method of sheepskin collagen oligopeptide

The technology of oligopeptide and collagen peptide is applied in the field of preparation of sheepskin collagen oligopeptide, which can solve the problems of easy environmental pollution and high viscosity, and achieve the effects of low cost, small molecular weight of oligopeptide and improved utilization rate.

Active Publication Date: 2019-10-29
内蒙古中科生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Patent CN104479995B discloses a method for extracting sheepskin collagen, in which acid and alkali reagents are used in the process of sheepskin treatment, and the production of a large amount of acid and alkali waste water in industrial production is likely to cause pollution to the environment
Although the average molecular weight of the obtained collagen is 1012Da and 937Da respectively, the viscosity is as high as 2.8mPa·S and 3.1mPa·S

Method used

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  • Preparation method of sheepskin collagen oligopeptide
  • Preparation method of sheepskin collagen oligopeptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] 1. For fresh sheepskin, use a shearing machine to cut the wool on the sheepskin to about 1cm.

[0036] 2. Use a meat remover to remove fat and meat from the sheepskin.

[0037] 3. Soak in hot water at 50°C for 40 minutes, and then use a hair removal machine to remove the remaining short hairs on the sheepskin.

[0038] 4. Use a crusher to break the sheepskin to about 1cm in diameter.

[0039] 5. Put the broken sheepskin into the reaction tank, add alkaline electrolyzed water twice the weight of the sheepskin, the pH of the electrolyzed water is 12.5, stir for 30 minutes to stabilize the pH of the feed liquid, raise the temperature to 80°C, and keep at 80°C 3 hours.

[0040] 6. Reduce the temperature in the tank to 45°C, add alkaline protease in an amount of 1.3% of the sheepskin weight, and perform enzymatic hydrolysis for 4 hours.

[0041] 7. Raise the temperature to 95°C and keep it for 20 minutes.

[0042] 8. Centrifuge with a decanter centrifuge to obtain a clar...

Embodiment 2

[0048] 1. For fresh sheepskin, use a shearing machine to cut the wool on the sheepskin to about 1cm.

[0049] 2. Use a meat remover to remove fat and meat from the sheepskin.

[0050] 3. Soak in hot water at 60°C for 20 minutes, and then use a hair removal machine to remove the remaining short hairs on the sheepskin.

[0051] 4. Use a crusher to break the sheepskin to about 1cm in diameter.

[0052] 5. Put the broken sheepskin into the reaction tank, add alkaline electrolyzed water three times the weight of the sheepskin, the pH of the electrolyzed water is 10.0, stir for 30 minutes to stabilize the pH of the feed liquid, raise the temperature to 75°C, and keep at 75°C 5 hours.

[0053] 6. Reduce the temperature in the tank to 55° C., add trypsin, and the amount of enzyme added is 0.8% of the sheepskin weight, and the enzyme is hydrolyzed for 5 hours.

[0054] 7. Raise the temperature to 90°C and keep it for 30 minutes.

[0055] 8. Centrifuge with a decanter centrifuge to ...

Embodiment 3

[0061] 1. For fresh sheepskin, use a shearing machine to cut the wool on the sheepskin to about 1cm.

[0062] 2. Use a meat remover to remove fat and meat from the sheepskin.

[0063] 3. Soak in hot water at 55°C for 30 minutes, and then use a hair removal machine to remove the remaining short hairs on the sheepskin.

[0064] 4. Use a crusher to break the sheepskin to about 1cm in diameter.

[0065] 5. Put the broken sheepskin into the reaction tank, add alkaline electrolyzed water 4 times the weight of the sheepskin, the pH of the electrolyzed water is 11.0, stir for 30 minutes to stabilize the pH of the feed liquid, raise the temperature to 80°C, and keep at 80°C 4 hours.

[0066] 6. Lower the temperature in the tank to 50°C, add a mixture of trypsin and alkaline protease (mass ratio 1:1), the total amount of enzyme added is 1.8% of the weight of sheepskin, and enzymatically hydrolyze for 3 hours.

[0067] 7. Raise the temperature to 95°C and keep it for 40 minutes.

[0...

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Abstract

The invention provides a preparation method of sheepskin collagen oligopeptide. The preparation method comprises the steps of with fresh sheepskin as a raw material, performing soaking with alkaline electrolysed water, performing enzymolysis treatment, performing anion-cation exchange resin adsorption, performing filtration, performing concentration, performing drying and the like so that the sheepskin collagen oligopeptide of which the molecular weight is wholly smaller than 1000Da is obtained. According to the preparation method, acid or alkali is not used, though anion-cation exchange resinadsorption, the pH of the oligopeptide is adjusted, and skin absorption is facilitated. In addition, the method is low in cost and environmentally-friendly, the oligopeptide molecular weight is small, and the preparation method has industrialization application prospects.

Description

technical field [0001] The invention relates to a preparation method of oligopeptide. More specifically, it relates to a preparation method of sheepskin collagen oligopeptide. Background technique [0002] Collagen is a biological polymer, the main component of animal connective tissue, and the most abundant and widely distributed functional protein in mammals, accounting for one-third of the total protein in mammals. As a natural degradable polymer, collagen has the advantages of high bioactivity, good biocompatibility, and appropriate degradation rate. [0003] Collagen peptides are hydrolyzed products of collagen. Generally, collagen peptides consisting of 2-10 amino acids are called oligopeptides, and oligopeptides can be absorbed and utilized by the human body without decomposition. With the growth of people's age and the influence of nutrition, sunshine, environment and other factors, the ability of the human body to synthesize collagen peptides gradually decreases,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/18
CPCC07K14/78C12P21/06
Inventor 王颖郭燕川王宏光王圣楠
Owner 内蒙古中科生物科技股份有限公司
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