High-throughput in-situ hybridization instrument and method

Abstract

The invention discloses a high-throughput in-situ hybridization instrument. The high-throughput in-situ hybridization instrument comprises a hybridization instrument body and a heating plate arrangedin the hybridization instrument body, a porous plate used for an in-situ hybridization reaction is detachably connected to the heating plate and provided with a plurality of spaced reaction holes which are independent mutually, the outer edge of the bottom of the porous plate extends downwards to form a positioning suction-fit structure, the positioning suction-fit structure comprises a hard annular plate and a telescopic annular wall which sequentially extend downwards from the bottom of the porous plate, a circle of sealing base is arranged at the bottom of the telescopic annular wall, a one-way valve hole in circulating direction uni-directionally towards the outside from an inner cavity defined by the hard annular plate is formed in the hard annular plate, when the porous plate is placed on the heating plate, the sealing base of the positioning suction-fit structure is attached to the heating plate, when the porous plate is pressed downwards, the telescopic annular wall is compressed to discharge air in the space defined by the telescopic annular wall and the heating plate from the one-way valve, and thus the porous plate and the heating plate are in suction fit.

Description

technical field [0001] The invention relates to the field of biotechnology, more specifically, it relates to a high-throughput in situ hybridization instrument and method. Background technique [0002] In situ hybridization is an emerging molecular cytogenetics technique. The principle is: according to the principle of nucleic acid base complementary pairing, hapten-labeled DNA or RNA probes are complementary paired with denatured single-stranded nucleic acid sequences, and the antibodies with fluorescent groups are used to recognize the hapten for detection, or fluorescent The group directly labels the probe and binds to the target sequence, and finally uses a fluorescence microscope to directly observe the distribution of the target sequence in the nucleus, chromosome or sliced ​​tissue. At present, this technology has been widely used in the study of genome structure of animals and plants, analysis of fine chromosome structure variation, analysis of virus infection, huma...

AI Technical Summary

Problems solved by technology

[0005] 1) The number of samples in a single test is small, that is, the throughput is low (12 samples / time);

[0006] 2) The amount of reagent used for each sample is relatively large;

[0007] 3) The hybridization solution is easy to dry out, which affects the results of the hybridization experiment

[0008] Therefore, the application of gene detection with tissue in situ hybridization instrument is limited to a certain extent.

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