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Culture medium comprising oligopeptides

A medium and oligopeptide technology, applied in cell culture medium, culture process, tissue culture, etc., can solve the problem that the medium is not optimal

Pending Publication Date: 2019-11-05
EVONIK DEGUSSA GMBH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0014] While the cell culture media described in the above prior art provide satisfactory performance and properties for certain cell culture processes, the prior art media are still not optimal

Method used

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  • Culture medium comprising oligopeptides
  • Culture medium comprising oligopeptides
  • Culture medium comprising oligopeptides

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Experimental program
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Effect test

Embodiment

[0128] General procedure

[0129] The effect of L-lysine dipeptide on antibody-producing Chinese hamster ovary (CHO) cells (subclone DG44; Life Technologies Cooperation, Carslbad) was studied compared with medium containing the corresponding free amino acid or the corresponding L-alanine dipeptide. , USA) growth. For this purpose, the cells were cultured in TC-42 medium produced by Xell AG, Bielefeld, Germany, which lacked the amino acids provided by the dipeptide in the experiments. The concentrations of these amino acids were adjusted so that, taking into account complete hydrolysis of the dipeptides to amino acids, the concentrations of all amino acids were the same in all media tested in this experiment.

[0130] Starting from a preparatory culture, adjust to 0.3 10 in shake flasks by adding test medium 6 Initial viable cell density (VCD) of cells / ml. After 2-4 days, transfer a portion of the cell culture to a new shake flask and adjust to 0.3 10 by adding new test medi...

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PUM

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Abstract

The invention relates to a culture medium, preferably a cell culture medium, comprising at least one oligopeptide of 2-10 amino acids in length, said amino acids being natural amino acids and at leastone of said amino acids being lysine (Lys) and one further amino acid is selected from cysteine (Cys), cystine (Cyss), leucine (Leu), tyrosine (Tyr), valine (Val) and Isoleucine (Ile) and the oligopeptide is present in an amount of at least 0.1 mM. The invention further relates to the use of a culture medium of the invention for culturing cells, preferably plant cells, animal cells or mammalian cells. Another aspect of the invention relates to a method of manufacturing a cell culture product comprising the steps of (i) providing a cell capable of producing said cell culture product; (ii) contacting said cell with a culture medium according to the invention; and (iii) obtaining said cell culture product from said culture medium or from said cell.

Description

[0001] field of invention [0002] The present invention relates to biotechnological production methods. More specifically, the present invention relates to improved media for use in biotechnological production processes, methods using such improved media and products obtained from such methods using improved media. [0003] Background of the invention [0004] Biotechnological methods are widely used in the production of biological products. These methods generally involve culturing the cells in a culture medium under conditions that permit growth of the cultured cells and product formation. Cells useful in biotechnological production are bacterial cells, fungal cells, yeast cells and cells of animal or plant origin. [0005] Animal cell cultures have long been used to produce biological products such as therapeutic proteins, polypeptides, oligopeptides or other biomolecules such as therapeutic polysaccharides. In this method of cell culture, animal cells, usually genetical...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/00
CPCC12N5/0031C12N5/005C12N2500/32C12N5/0018C12N2510/02
Inventor G·纳奥普F·梅茨C·凯斯勒
Owner EVONIK DEGUSSA GMBH