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A biological control method for cherry and/or tomato fruit postpartum mold rot

A fruit and cherry technology, applied in the field of biological control of plant diseases, can solve the problems of poor control effect, unsafe chemical preservatives for fruit, and high cost, and achieve the effects of convenient operation, significant control effect, and low production cost

Active Publication Date: 2020-11-27
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the current technical problems of preventing cherry and tomato fruit mildew and rot in transportation and / or storage, special equipment and conditions are required, the cost is high, the chemical preservatives are unsafe for fruits, and the control effect is poor. The present invention provides The following biological control methods and bacterial strains of cherry and / or tomato fruit postpartum mildew

Method used

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  • A biological control method for cherry and/or tomato fruit postpartum mold rot
  • A biological control method for cherry and/or tomato fruit postpartum mold rot
  • A biological control method for cherry and/or tomato fruit postpartum mold rot

Examples

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Embodiment 1

[0023] Example 1 Isolation, purification, identification and preservation of Bacillus mucilaginosus strain YN2011 of the present invention

[0024] 1 Isolation and purification of Bacillus colioids strain YN2011

[0025] 1.1 Materials

[0026] Harvested from field soil where tobacco is grown.

[0027] 1.2 Culture medium preparation

[0028] The solid medium for silicate bacteria is: sucrose 5.0g / L, sodium phosphate 2.0g / L, magnesium sulfate heptahydrate 0.5g / L, calcium carbonate 0.1g / L, ferric chloride hexahydrate 5.0mg / L, agar powder 15g / L, pH 7.0.

[0029] Melt the above-mentioned silicate bacteria solid culture medium, pour them into sterilized petri dishes respectively, cool down, and make flat plates for separating and preserving bacteria.

[0030] 1.3 Isolation and culture

[0031] Under sterile conditions, weigh 10 g of the field soil sample collected from planting tobacco, dissolve it in 90 mL of sterile water, shake it fully, and dilute it gradually to 10 -2 、10...

Embodiment 2

[0041] The preparation of embodiment 2 microbial preparations of the present invention comprises the following steps:

[0042] (1) Connect 1 to 2 loops of the Bacillus mucilaginosus strain YN2011 stored at -80°C to the seed medium, and cultivate at 32°C for 24 hours to obtain activated Bacillus mucilaginosus strain YN2011, press 1% inoculum size, inoculate the activated colloid bacillus (Bacillus mucilaginosus) bacterial strain YN2011 into the seed culture medium, under the condition of 32 ℃ of culture temperature, rotating speed 160rpm, shake table culture 12h, obtain first-class seed liquid, described seed culture The composition of the base is: sucrose 8g / L, yeast extract 0.7g / L, ammonium sulfate 1.5g / L, calcium carbonate 1g / L, magnesium sulfate heptahydrate 0.8g / L, dipotassium hydrogen phosphate 2g / L, pH 7.5 .

[0043] (2) Transfer the primary seed solution to the secondary seed medium in the seed tank according to 2% inoculum amount, and cultivate it at 32°C, with a rota...

Embodiment 3

[0046] Embodiment 3 The application of microbial preparation soaking treatment of the present invention to prevent and treat the fruit mold and rot of big cherry Brooks

[0047] 1. Test period: May 2019-June 2019.

[0048] 2. Test location: the key laboratory of Yunnan Agricultural University.

[0049] 3. Test material: The variety of the large cherry to be tested is Brooks, and the ripe and well-ripe large cherry Brooks was picked on the same day.

[0050] 4. with the microbial preparation of the present invention prepared in embodiment 2, the effective viable count of its colloid bacillus (Bacillus mucilaginosus) bacterial strain YN2011 fermented liquid is 1 * 10 8 cfu / ml, referred to as: product stock solution, and dilute the product stock solution with sterile water to 10 times, 100 times, a total of 3 concentration treatments, when soaking the fruit of Big Cherry Brooks in each treatment, the whole fruit is soaked for 10 minutes, and then Rinse with water for 5 minutes....

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Abstract

The invention discloses a biological control method for cherry and / or tomato fruit postpartum mildew and rot, and relates to the technical field of plant disease biological control. The bacterial strain used in the biological control is Bacillus mucilaginosus strain YN2011, and its preservation number is CGMCC No.5724. The microbial preparation of the present invention contains the bacterial strain, and the microbial preparation is used to soak cherries and tomato fruits for 10 minutes, store at 2°C-25°C for 30 days, and the relative control effect on postpartum mildew of cherries and tomato fruits reaches 100%. The method and the product of the invention have low production cost, convenient operation, no poison, no pollution and no residue, and are an environment-friendly method, bacterial strain and microbial preparation.

Description

technical field [0001] The invention relates to the technical field of biological control of plant diseases, in particular to a colloid bacillus and its biological control of cherry and / or tomato fruit mildew and rot during transportation and storage. [0002] technical background [0003] Cherry and tomato fruit mildew is a common infectious disease that occurs during transportation and storage of cherry and tomato fruit. Cherry and tomato fruits have been exposed to the air throughout the growth process and are threatened by various molds. Because cherry and tomato fruits are in the late growth stage, the fruits are rich in various nutrients and are very easy to breed various microorganisms. During the transportation, storage and storage of cherries and tomato fruits, once the conditions are suitable, the cherries and tomato fruits are prone to mildew and deterioration, basically losing their utilization value, and bringing great economic losses to the producers and distrib...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23B7/155C12R1/07
CPCA23B7/155C12N1/20C12N1/205C12R2001/07
Inventor 何鹏搏何鹏飞吴毅歆沈艳卜姝文赵德柱
Owner YUNNAN AGRICULTURAL UNIVERSITY
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