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Microbial antibacterial agent and preparation method thereof

A bacteriostatic and microbial technology, applied in the field of microorganisms, can solve problems such as food safety, environmental problems, and excessive soil pesticide residues, and achieve the effects of safe use, low cost, and structural change

Inactive Publication Date: 2019-11-15
GANSU AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the existing technology, farmers focus on improving the high yield and gain of oats, and excessively use chemical pesticides to prevent and control the occurrence of pests and diseases. For a long time, harmful substances in the land have accumulated day by day, resulting in excessive pesticide residues in the soil, environmental problems and food safety problems.

Method used

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  • Microbial antibacterial agent and preparation method thereof
  • Microbial antibacterial agent and preparation method thereof
  • Microbial antibacterial agent and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0025] In order to describe in detail the technical content, objectives and effects of the present invention, the following will be described in conjunction with the embodiments.

[0026] A kind of microbial bacteriostasis agent, comprises: mixed bacteria solution and auxiliary agent, mixed bacteria solution is the mixed liquid of Bacillus mycoides Gnyt1 and Pseudomonas sp.ChO19, and auxiliary agent comprises Tween-80 , Sorbitol, Methylparaben.

[0027] It should be noted that Bacillus can produce spores that are highly resistant to heat, ultraviolet rays, ionizing radiation and certain chemicals, and can tolerate various adverse environments, which is beneficial to the production of biocontrol agents, formulation processing and Survive and reproduce in the environment; Tween-80, also known as polysorbate-80, is a non-ionic hydrophilic surfactant with a slight peculiar smell, slightly bitter taste, slightly astringent, warm feeling, and can be used as an injection Solubilizer...

Embodiment 2

[0033]A kind of preparation method of microbial antibacterial agent, see figure 1 , the method includes the following steps:

[0034] Step (1), preparing a mixed bacterial solution: respectively culturing Bacillus mycoides Gnyt1 and Pseudomonas sp.ChO19, and then mixing them to obtain a mixed bacterial solution.

[0035] Step (2), determining the ratio of auxiliary agents: based on the mixed bacterial solution, the ratio of auxiliary agents is determined by response surface analysis.

[0036] Step (3), preparing the microbial bacteriostatic agent: mixing the prepared mixed bacterial liquid with the auxiliary agent, and then culturing to obtain the microbial bacteriostatic agent.

Embodiment 3

[0038] A kind of preparation method of microbial bacteriostatic agent, combines figure 2 , for the above figure 1 The preparation method of shown microbial bacteriostat is discussed in detail:

[0039] Step (101): Bacillus mycoides Gnyt1 and Pseudomonas sp.ChO19 were respectively inoculated in LB solid medium by streaking method, and placed in an incubator at 28° C. for 3 days.

[0040] It should be noted that the streaking method refers to the dilution of microorganisms mixed together or different cells in the same microbial population with an inoculation loop on the surface of the plate medium to obtain more independently distributed single cells, which grow after culture. Propagate into a single colony, which is usually regarded as a pure species of the microorganism to be isolated. Sometimes this single colony is not all reproduced from a single cell, so it must be repeatedly isolated many times to obtain a pure species. The principle is to dilute microbial samples on ...

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PUM

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Abstract

The invention discloses a microbial antibacterial agent and a preparation method thereof, and belongs to the technical field of microbes. The microbial antibacterial agent includes a mixed bacterial liquid and assistants, the mixed bacterial liquid is a liquid obtained by mixing Bacillus mycoides Gnyt1 with Pseudomonas sp.ChO19, and the assistants include Tween 80, sorbitol and methylparaben. Theabove two strains and the assistants have synergistic effects, so the microbial antibacterial agent can prevent diseases and promote the growth, and also has good wetting, antiseptic, dispersing and ultraviolet-protecting properties.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a microbial antibacterial agent and a preparation method thereof. Background technique [0002] Oat is a grass plant, which is called brome and wild wheat in "Compendium of Materia Medica". Oatmeal is not easy to peel, so it is called oatmeal with skin. It is a low-sugar, high-nutrition, high-energy food. Oatmeal is sweet and flat in nature, can nourish the spleen and heart, and suppress sweating. It has high nutritional value and can be used for spontaneous sweating, night sweats or tuberculosis patients. Oats are cold-resistant, drought-resistant, highly adaptable to the soil, and can reproduce by themselves. Oatmeal is rich in dietary fiber, which can promote gastrointestinal motility, facilitate defecation, low calorie, low glycemic index, lower fat and blood sugar, and is also one of the high-end supplements. It is an indispensable dry food in poor areas. [0003] ...

Claims

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Application Information

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IPC IPC(8): A01N63/02A01P3/00C12N1/20C12R1/07C12R1/38
CPCC12N1/20
Inventor 姚拓张建贵赵桂琴侯栋胡彦婷杨琰珊董凯
Owner GANSU AGRI UNIV
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