Method of evaluating developmental toxicity of polybrominated diphenyl ethers

A polybrominated diphenyl ether and toxicity technology, applied in the field of toxicology detection, can solve the problems of intuition and high research cost, and achieve the effects of easy observation, reduction of test cost, and improvement of efficiency

Inactive Publication Date: 2019-11-19
HUAIYIN TEACHERS COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Experimental research requires a special zebrafish juvenile behavior recording and analysis system, which is expensive and not intuitive

Method used

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  • Method of evaluating developmental toxicity of polybrominated diphenyl ethers
  • Method of evaluating developmental toxicity of polybrominated diphenyl ethers
  • Method of evaluating developmental toxicity of polybrominated diphenyl ethers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Zebrafish Feeding Management

[0034] (1) Zebrafish rearing: zebrafish are raised in a culture tank with a constant temperature circulating water system, wherein the culture water in the culture tank is tap water heated by aeration, and the water temperature in the culture tank is 28±0.5 ℃, the dissolved oxygen in the water is greater than 6.0mg / L, the pH is 7.0-8.0, and the light cycle of 14h light / 10h dark is maintained. Freshly hatched Artemia were fed from 7:00 to 9:00, 14:00 to 15:00, and 19:00 to 20:00 every day.

[0035] (2) Breeding method of Artemia: add 1000mL of water, 20g of sea salt, 0.7g of NaHCO to the hatching bottle 3 And 2.5g of Artemia eggs, put it into an artificial climate box, and maintain a constant temperature of 28±0.5℃. Open the air valve and inflate the device, so that the eggs of Artemia are in a state of tumbling motion, and the incubation time is 24-30 hours. Close the air valve and let it stand until the Artemia is fully charged to the ...

Embodiment 2

[0037] Selection of zebrafish embryos

[0038] (1) At 8:30 the night before egg retrieval, use a fishing net to move the adult zebrafish to the spawning box in a ratio of 1:1, and the water in the spawning tank is zebrafish breeding water. The spawning box was placed in an artificial climate box at 28 ± 0.5 °C for overnight incubation, and the light was turned on at 6:30-7:30 the next morning to allow the female and male to mate naturally, and fertilized eggs were collected after 15 minutes.

[0039] (2) Place the fertilized eggs in a plate, and use a plastic-tip dropper to clean up feces and impurities. Select normally developing embryos under a stereoscope, rinse with embryo culture medium twice, add embryo culture medium and transfer to culture in an artificial climate box at 28±0.5°C for 4h.

Embodiment 3

[0041] Preparation of BDE-47 solutions with different concentrations:

[0042] (1) Mother liquor preparation: Dissolve 25mg BDE-47 in 5mL dimethyl sulfoxide (DMSO), add 495mL embryo culture medium, and prepare BDE-47 mother liquor (50mg / L);

[0043] (2) Preparation of stock solution: Take 100 mL of BDE-47 stock solution and add 900 mL of embryo culture medium to prepare BDE-47 stock solution (5 mg / L).

[0044] (3) Working solution preparation: take 25 mL of BDE-47 stock solution, add 75 mL of embryo culture medium, and prepare a BDE-47 solution with a concentration of 1.25 mg / L. Take equal volumes of 1.25mg / L BDE-47 solution and embryo culture medium, and mix to prepare 0.625mg / L BDE-47 solution. Take equal volumes of 0.625 mg / L BDE-47 solution and embryo culture medium, and mix them to prepare 0.312 mg / L BDE-47 solution.

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Abstract

The invention provides a method of evaluating the developmental toxicity of polybrominated diphenyl ethers, which relates to the technical field of toxicology. The method includes the following steps:normal developing zebrafish embryos are placed in polybrominated diphenyl ether solutions of different concentrations for cultivation for 44 to 68 h as an experiment group, a blank control group is set at the same time, and the zebrafish embryo pigment change is compared; and the embryo pigment concentration in the experiment group is significantly reduced in comparison with the blank control group, suffering from the developmental toxicity of polybrominated diphenyl ethers is proved. The method provided in the invention uses the zebrafish as a test object to detect the polybrominated diphenyl ethers, a large number of test animals with good homogeneity can be acquired quickly, the test cost is reduced, the symptoms of poisoning can be observed easily, the fish pigment change can be observed intuitively in real time, and the efficiency, the intuitiveness and the accuracy of polybrominated diphenyl ether detection are improved.

Description

technical field [0001] The invention belongs to the technical field of toxicology detection, in particular to a method for evaluating the developmental toxicity of polybrominated diphenyl ethers. Background technique [0002] Polybrominated diphenyl ethers (PBDEs) are persistent organic pollutants that are widespread in the environment. Due to their excellent flame retardant properties, PBDEs are widely used in electronic equipment, textiles, plastics and building and decoration materials. Because PBDEs are non-chemically bound to the carrier, during use and disposal, PBDEs can migrate from products to environmental media through various routes, and can enter organisms through food, inhalation, and skin contact. PBDEs have high lipid solubility and long degradation cycle, so they can accumulate in the environment and in vivo, posing a threat to the ecological environment and human health. Toxicological studies have shown that PBDEs have thyroid toxicity, liver developmenta...

Claims

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Application Information

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IPC IPC(8): G01N21/75
CPCG01N21/75
Inventor 庄娟潘正军张佳琪汤潇
Owner HUAIYIN TEACHERS COLLEGE
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