A strain of Lactobacillus plantarum and its application

A technology of Lactobacillus plantarum and live bacteria, applied in the direction of Lactobacillus, application, bacteria, etc., can solve the problems of low lactic acid bacteria, single effect, etc., to enhance immunity, improve immune cell immune activity, good acid and bile salt tolerance sexual effect

Active Publication Date: 2021-06-04
诺佰克(武汉)生物科技有限公司
View PDF8 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the lactic acid bacteria disclosed in the prior art have a relatively single effect of improving the immunity of the body, lacking a kind of lactic acid bacteria that can not only improve the immunity of the normal body, but also enhance the immunity of the body with low immunity.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A strain of Lactobacillus plantarum and its application
  • A strain of Lactobacillus plantarum and its application
  • A strain of Lactobacillus plantarum and its application

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0034] The present invention also provides a preparation method for the preparation described in the above scheme, comprising the following steps:

[0035] 1) inoculating Lactobacillus plantarum nbk-BC299 into the seed medium and activating it to obtain a first-grade seed liquid;

[0036] 2) inoculating the primary seed liquid described in step 1) into the seed medium, and culturing to obtain the secondary seed liquid;

[0037] 3) inoculating the secondary seed liquid in step 2) into the fermentation medium, carrying out fermentation, and centrifuging the fermentation liquid to obtain thallus;

[0038] 4) Drying and pulverizing the cells described in step 3) to obtain a preparation.

[0039] In the present invention, firstly, Lactobacillus plantarum is inoculated into the seed medium and activated to obtain a first-grade seed liquid; the present invention has no special limitation on the inoculum amount of the Lactobacillus plantarum; the seed medium preferably includes the f...

Embodiment 1

[0052] Screening and identification of embodiment 1 Lactobacillus plantarum nbk-BC299

[0053] Naturally fermented sour cabbage (collected from the homes of local residents in Wuhan) was collected, and after grinding, it was diluted with sterile saline to an appropriate gradient (10 3 ~10 7 ), were streaked on the MRS agar plate using the four-section line method, and cultured anaerobically in a 37°C incubator for 48h. A single colony was selected, examined under a microscope, streaked, and further purified to obtain Lactobacillus plantarum nbk-BC299. After identification (the identification method is 16S full-length sequencing), it is Lactobacillus plantarum and named as Lactobacillus plantarum nbk-BC299.

Embodiment 2

[0054] Embodiment 2 A kind of preparation method comprising the bacteria powder of plantaractobacillus nbk-BC299 live bacteria

[0055] In terms of mass percentage, seed medium: 2% glucose, 1.2% peptone, 0.5% yeast extract, 0.5% anhydrous sodium acetate, 0.4% ammonium citrate, 0.02% magnesium sulfate, 0.06% manganese sulfate, 0.1% Tween 80 and the balance of water, pH 6.5.

[0056] By mass percentage, fermentation medium: 2% glucose, 1% peptone, 0.5% yeast extract, 0.5% anhydrous sodium acetate, 0.2% ammonium citrate, 0.06% magnesium sulfate, 0.02% manganese sulfate, 0.1% Tween 80 and the balance of water with a pH of 6.4.

[0057] In parts by mass, the lyoprotectant: 2 parts of 10% skim milk, 2 parts of 3% glycerin and 5 parts of water.

[0058] Preparation

[0059] 1) Inoculate Lactobacillus plantarum into a test tube containing seed medium for activation (37°C, 16h) to obtain a first-grade seed liquid;

[0060] 2) Transfer the primary seed solution to the seed medium ac...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
particle size (mesh)aaaaaaaaaa
Login to view more

Abstract

The invention provides a strain of plant lactobacillus and its application, and belongs to the technical field of new strains of the genus Lactobacillus and its application. The Lactobacillus plantarum (Lactobacillus plantarum) nbk-BC299 of the present invention has a preservation number of CGMCC NO.16871. Both the bacteria powder and the inactivated bacteria powder of Lactobacillus plantarum nbk-BC299 of the present invention can effectively increase the weight of mice; all can improve mouse specific cellular immunity, specific humoral immunity and non-specific immunity. Rat immunity; both can improve the immune activity of immune cells, increase the content of antibodies and various cytokines in serum, and effectively enhance the immunity of immunocompromised mice; in addition, Lactobacillus plantarum nbk‑BC299 has good acid and bile salt tolerance Receptivity, which can ensure a sufficient number of live bacteria reach the intestinal tract and play a probiotic role.

Description

technical field [0001] The invention relates to the technical field of new bacterial strains of the genus Lactobacillus and the application thereof, in particular to a strain of Lactobacillus plantarum and the application thereof. Background technique [0002] With the increasing pressure of life and the irrationalization of diet and rest, most of the people's physical condition is in a sub-health state. Improving immunity has an important impact on getting rid of sub-health status and preventing diseases. There are many factors that affect the decline of the body's immunity, mainly in the destruction of the first barrier between the body's skin and mucous membranes and the body's immunity; one's own psychological factors, negative and pessimistic personality will affect the body's immune system, resulting in low immunity; Lack of exercise and excessive fatigue can also affect the immune system; aging and aging of the body cause the body's immune response to decline; unreas...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23K10/18A23K10/16A23L33/135A61K35/747A61P37/04C12R1/25
CPCC12N1/20A23K10/18A23K10/16A23L33/135A61P37/04A23V2002/00A61K2035/115C12R2001/25C12N1/205A23V2400/169A23V2200/324A23V2200/3204
Inventor 张阳潘芬湛剑龙蒋彪
Owner 诺佰克(武汉)生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap