Quality control method of podocarpus macrophyllus fruit medicinal material

A quality control method, a loose technology, applied in the direction of measuring devices, material separation, analysis materials, etc., can solve problems that do not involve the quality control method of Podocarpus pine

Active Publication Date: 2019-11-22
GUANGXI MEDICAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

All these patents do not involve the quality control method of Podocarpus medicinal material

Method used

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  • Quality control method of podocarpus macrophyllus fruit medicinal material
  • Quality control method of podocarpus macrophyllus fruit medicinal material
  • Quality control method of podocarpus macrophyllus fruit medicinal material

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] TLC identification

[0022] Take about 20 mg of the Ligustrazine reference substance, weigh it accurately, add methanol to dissolve it to make a reference solution containing 1 mg of Ligustrazine per 1 ml; take another 200 g of dried Podocarpus powder, pass through a 20-mesh sieve, and extract with 95% ethanol under reflux 2 times, 500ml each time, extracting for 1 hour each time, combining the two extracts, recovering ethanol under reduced pressure at 60°C to obtain the extract, drying in vacuum at 80°C to obtain a dry paste, taking about 20mg of the dry paste, accurately weighing, adding Dissolve 50ml of methanol, add 1g of activated carbon, ultrasonically treat for 60 minutes, filter, evaporate the filtrate to dryness, add methanol to dilute to 1ml, and prepare the test solution;

[0023] Take 5 μl each of the reference substance solution and the test solution, and place them on the same silica gel G thin-layer plate, use the solution of cyclohexane:ethyl acetate (2:...

Embodiment 2

[0025] Assay

[0026] 1 Instruments and materials

[0027] Agilent1260 high performance liquid chromatograph (Agilent Corporation, USA), electronic balance (Mettler-Torley Instrument Co., Ltd., Switzerland), XS-205DU one-hundred-thousandth balance (Mettler-Torley Instrument Co., Ltd., Switzerland), KQ -500DB CNC ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd.), CD-UPH-II-20T ultrapure water device (Chengdu Ultrapure Technology Co., Ltd.), universal pulverizer (Wuxi Jiuping Instrument Co., Ltd.).

[0028] The fresh podocarpus is collected from Beihai, Guangxi, and is identified as the fruit of Podocarpus brevifolia P.macrophyllus (Thunb.) D.Don var.maki Endl. Ligustrazine reference substance (Shanghai Yaji Biotechnology Co., Ltd.), methanol was chromatographically pure, phosphoric acid, petroleum ether and anhydrous sodium sulfate were all analytically pure, and water was deionized water.

[0029] 2 Methods and results

[0030] 2.1 Preparation of solution

[003...

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Abstract

The invention discloses a quality control method of a podocarpus macrophyllus fruit medicinal material. The quality control method is characterized in that a chemical component ligustrazine in the medicinal material is taken as a quality control index, a method for identifying ligustrazine in the medicinal material by means of thin-layer chromatography and a method for determining the content of ligustrazine in the medicinal material by means of high performance liquid chromatography are established. The result of the established thin-layer identification method shows that spots are clear, andinterference of other components does not exist; the high performance liquid chromatography target peak has high resolution, good peak shape, high specificity, good reproducibility, high precision and universal applicability and is free of interference of other impurity peaks. The quality control method can scientifically and effectively control the quality of the podocarpus macrophyllus fruit medicinal material.

Description

technical field [0001] The invention belongs to the fields of traditional Chinese pharmacy, natural medicine chemistry, traditional Chinese medicine analysis and medical technology, and in particular relates to a quality control method of podocarpus medicinal material. Background technique [0002] Podocarpus is a plant of the Podocarpus family Podocarpus, mainly distributed in Jiangsu, Zhejiang, Fujian, Hunan, Hubei, Jiangxi, Sichuan, Guizhou, Yunnan, Guangdong and Guangxi in China, with a planting area of ​​15,000 mu in Beihai City, Guangxi. Podocarpus fruit is the fruit of Podocarpus macrophyllus (Thunb.) D.Don or Podocarpus macrophyllus (Thunb.) D.Don var.maki (Sieb.) Endl., consisting of seeds and receptacles. According to the "Dictionary of Chinese Medicine", Podocarpus is a kind of medicinal material, which has the effects of nourishing the kidney and lung, treating blood deficiency, sallow complexion and heart and stomach pain. According to research, at present, Pod...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/90
CPCG01N30/02G01N30/06G01N30/90
Inventor 黄增琼曾凡艳林乐珍杜坤刘小杜
Owner GUANGXI MEDICAL UNIVERSITY
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