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Preparation method and application of hosta plantaginea root animal-origin-fungi-resistant effective parts

An animal-derived, white-flowered technology, applied in the field of ethnic medicine, can solve the problems of large differences in active ingredients and unpredictable antifungal activity of hosta roots.

Inactive Publication Date: 2019-12-06
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the large difference in the active ingredients of different medicinal parts, the antifungal activity of hosta root is unpredictable

Method used

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  • Preparation method and application of hosta plantaginea root animal-origin-fungi-resistant effective parts
  • Preparation method and application of hosta plantaginea root animal-origin-fungi-resistant effective parts
  • Preparation method and application of hosta plantaginea root animal-origin-fungi-resistant effective parts

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Preparation method of each effective part:

[0024] 1) Total extract extraction

[0025] Take 300g of dried white hosta roots, dry, and crush. Soak the powder of hosta roots with 10 times the amount of 70% ethanol for half an hour.

[0026] 2) Reflux extraction

[0027] Reflux and extract twice at 70-80°C for two hours each time, filter, collect the filtrate, and concentrate to 200 mL.

[0028] 3) Gradient extraction

[0029] The diluted concentrate was extracted with petroleum ether, chloroform, ethyl acetate, and n-butanol in turn, combined the same organic phases, and concentrated under reduced pressure to obtain petroleum ether fraction (0.2074 g, extraction rate: 0.0691%) and chloroform fraction (1.5708 g , Extraction rate: 0.524%), ethyl acetate fraction (0.8724 g, extraction rate: 0.291%), n-butanol fraction (20.5678 g, extraction rate: 6.86%) and water fraction (42.0368 g, extraction rate: 14%) .

Embodiment 2

[0031] The Ethanol Extract from the Root of Hosta White Flower and Its Different Polar Parts' Antifungal Effects on Animal Origin

[0032] 1) Resuscitation of strains: Inoculate Microsporum canis, Trichophyton mentagrophytes, and Microsporum gypsum stored in a refrigerator at 4°C into PDA medium, and place it in a fungus constant temperature incubator at 27°C for 7 days to activate. , Pick the mycelium around the activated colony and inoculate it into the PDA medium, and place it in a fungus incubator at 25°C for 7 days to restore the fungus to normal growth.

[0033] 2) Determination of the antifungal activity of the ethanol extract from the roots of Hosta white flower and its different polar parts: the ethanol extract and the four polar parts in Example 1 were dissolved in 500 μL of sterile distilled water and then diluted with distilled water to 50 mg / mL. In the ultra-clean workbench, after the aqueous solution is filtered through a microporous membrane (0.22 μm), add the melted...

Embodiment 3

[0038] MIC Determination of Ethanol Extracts from the Roots of Hosta White Flower and Its Different Polar Parts against Animal-derived Fungi

[0039] In a 96-well plate, add 100 μL of freshly prepared PDB medium to wells 1 to 6, add 100 μL (20 mg / mL) of the prepared drug solution to well 1, and use the multiple dilution method to dilute the drug in wells 2 to 6 . Add 100 μL of freshly prepared bacterial solution to wells 1~6 (10 6 CFU / mL). Add only 200 μL of bacterial solution to the 9th well, 200 μL of liquid medium to the 10th well, 100 μL of LPDB medium and 100 μL of bacterial to the 11th hole, and 100 μL of LPDB medium and 100 μL of medicine to the 12th hole. Solution, respectively, as controls. After placing each plate in a 35°C incubator for 48 hours, compare with the control group to observe the inhibitory effect of the drug on the growth of the tested bacteria.

[0040] See the results of the experiment Figure 4-Figure 8 .

[0041] See the results of MIC determination of...

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Abstract

The invention relates to hosta plantaginea root extract effective parts with a fungi-resistant effect and a preparation method of the hosta plantaginea root extract effective parts. The effective parts are selected from a hosta plantaginea root ethyl alcohol extract and one and a combination of a petroleum ether part, a chloroform part, an ethyl acetate part and an n-butanol part. Study proves that the petroleum ether part, the chloroform part and the n-butanol part have the obvious in-vitro animal-origin-fungi-resistant capacity, and thus the effective parts can be applied to skin fungal diseases.

Description

Technical field [0001] The present invention relates to an effective part of hosta root ethanol extract and a preparation method thereof; the present invention further relates to a drug combination of the effective part; the present invention also relates to the application of the effective part in an in vitro anti-animal-derived fungal medicine; Ethnic medicine field. Background technique [0002] In recent years, affected by environmental pollution and ecosystem imbalance, conflicts between man and nature have become more frequent. Mankind is faced with serious problems such as the toxic and side effects of chemical drugs and the increasing number of iatrogenic diseases. The voice of "advocating nature and returning to the basics" is increasing. People's social demand for natural medicines and natural health products is increasing. Mongolian medicine is an important part of traditional medicine in the world. It is the result of continuous creation, accumulation and selection o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/896A61P31/10A61P17/00A61K125/00
CPCA61K36/896A61K2236/333A61K2236/35A61K2236/39A61P17/00A61P31/10
Inventor 梁晓霞胡云锴吴强王昊王涵杨星月冯长青黄思慧
Owner SICHUAN AGRI UNIV
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