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Organic acid inhibitor resistant to fish disease, and preparation method and application thereof

An organic acid and inhibitor technology, which is applied in the field of organic acid inhibitors against fish diseases and its preparation, can solve the problems of no drugs available, and achieve the effects of reducing drug residues, improving production efficiency, and reducing dosage

Active Publication Date: 2019-12-10
GUANGXI ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no marketable effective antiviral drug against grouper iridescent virus disease, and the grouper aquaculture industry is in the predicament of no drug available

Method used

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  • Organic acid inhibitor resistant to fish disease, and preparation method and application thereof
  • Organic acid inhibitor resistant to fish disease, and preparation method and application thereof
  • Organic acid inhibitor resistant to fish disease, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] 1. Main instruments and reagents

[0034] Optical microscope.

[0035] 2. Experimental method

[0036]GS cells were introduced into 96-well plates at the number of 10,000 / well, cultured at 28°C for 24 hours, and then the four plant sources of isochlorogenic acid A, isochlorogenic acid B, isochlorogenic acid C and caffeic acid Compound monomers were diluted to different concentrations in cell culture medium (isochlorogenic acid A: 1000, 500, 250 μg / mL; isochlorogenic acid B: 1000, 500, 250 μg / mL; isochlorogenic acid C: 2000, 1000, 500 μg / mL; caffeic acid: 500, 250, 125 μg / mL); in the experimental group, different concentrations of isochlorogenic acid A, isochlorogenic acid B, isochlorogenic acid C and caffeic acid were mixed with 96 wells The GS cells in the plate were incubated at 28°C for 48 hours, and the cell morphology was observed with a light microscope. The control group in this experiment was GS cells without adding the above-mentioned plant-derived compound ...

Embodiment 2

[0040] 1. Main instruments and reagents

[0041] Microplate reader, 5-(2,4-disulfophenyl)-3-(2-methoxy-4-nitrophenyl)-2H-tetrazole sodium inner salt solution (WST-8 solution).

[0042] 2. Experimental method

[0043] GS cells were introduced into 96-well plates at the number of 10,000 / well, cultured at 28°C for 24 hours, and then the four plant sources of isochlorogenic acid A, isochlorogenic acid B, isochlorogenic acid C and caffeic acid Compound monomers were set to different concentrations in the cell culture medium (isochlorogenic acid A: 2000, 1000, 500, 250, 125 μg / mL; isochlorogenic acid B: 2000, 1000, 500, 250, 125 μg / mL; Isochlorogenic acid C: 2000, 1000, 500, 25, 125 μg / mL; caffeic acid: 2000, 1000, 500, 250, 125, 62.5 μg / mL); different concentrations of plant-derived compound monomers were mixed with 96 wells The GS cells in the plate were incubated at 28°C for 48h. In order to detect cell viability, 20 μL of WST-8 solution was added to the cells in each well to ...

Embodiment 3

[0049] 1. Main instruments and reagents

[0050] Real-time fluorescent quantitative PCR technology (qRT-PCR).

[0051] 2. Experimental method

[0052] Dilute isochlorogenic acid A, isochlorogenic acid B, isochlorogenic acid C and caffeic acid in the cell culture medium and set to different concentrations (isochlorogenic acid A: 250, 125, 62.5 μg / mL; isochlorogenic acid B: 250, 125, 62.5 μg / mL; isochlorogenic acid C: 500, 250, 125 μg / mL; caffeic acid: 125, 62.5, 31.25 μg / mL) plant compound monomer solution, The RT-qPCR technique was used to detect the effect of the plant compound monomer solution prepared in this example on inhibiting grouper iridescent virus infection under different concentration conditions. The GS cells were introduced into a 24-well plate at a quantity of 40,000 / well, and cultured at 28°C for 24 hours. The plant compound monomer solution to be tested was mixed with SGIV-Gx virus (10 5 TCID 50 / mL) were added to the cells in a 24-well plate and incubat...

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PUM

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Abstract

The invention provides an organic acid inhibitor resistant to a fish disease. The organic acid inhibitor comprises at least one of isochlorogenic acid A, isochlorogenic acid B, isochlorogenic acid C and caffeic acid; the mass ratio of the isochlorogenic acid A, the isochlorogenic acid B, the isochlorogenic acid C and the caffeic acid is 0-4:0-4:0-4:0-1. The organic acid inhibitor resistant to thefish disease can be used for preventing and inhibiting Singapore grouper iridovirus safely and effectively, and meets the requirements of green and pollution-free sustainable development; and high quality ecological culture of aquaculture can be promoted, and non-negligible economic benefits are provided.

Description

technical field [0001] The invention belongs to the field of prevention and treatment of aquatic animal diseases, and specifically designs an organic acid inhibitor for resisting fish diseases and its preparation method and application. Background technique [0002] In recent years, my country's marine economy has developed rapidly. The "2017 China Marine Economic Statistical Bulletin" data shows that the national gross marine product reached 7.76 trillion yuan in 2017, of which the marine fishery economy accounted for more than 17.8% of the marine economy. Grouper is delicate in meat and rich in nutrition. As one of the largest marine fishes in South my country, the grouper has a very high economic value in the live seafood market. However, under high-density and intensive farming conditions, frequent outbreaks of various breeding diseases have caused huge economic losses. Among them, the grouper iridescent virus is called "marine foot-and-mouth disease", and the fatality ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/216A61K31/192A61P31/20
CPCA61K31/216A61K31/192A61P31/20A61K2300/00Y02A50/30
Inventor 李鹏飞余庆刘明珠肖贺贺德蒂.费兹瑞恩塞亚.普察
Owner GUANGXI ACAD OF SCI
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