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Method for extracting soy isoflavone aglycone from soybean milk water

A technology for isoflavone aglycone and soybean milk, applied in the field of extracting soybean isoflavone aglycone, can solve the problems of low purity and complexity of consumption of soybean isoflavone glycoside and soybean isoflavone aglycone, and achieve improved purity, low purity and large investment. Effect

Inactive Publication Date: 2019-12-10
SHANDONG YUWANG ECOLOGY FOOD IND +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method is relatively complicated, the purity of the prepared soy isoflavone aglycone is low, and it needs to consume soy isoflavone aglycone

Method used

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  • Method for extracting soy isoflavone aglycone from soybean milk water

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Take the alkaline extraction and acid precipitation process to produce 4L of soy milk water discharged from the soybean protein, and naturally settle for 4h to obtain 1000 mL of yellow liquid in the lower layer (the supernatant is colorless). Add 15g of activated clay to the liquid, magnetically stir for 0.5h, and filter to obtain activated clay residue. After natural drying or drying (<140℃), add 30mL of ethyl acetate, magnetically stir for 60min, and centrifuge at 5000r / min for 12min. Take the ethyl acetate supernatant and spin-evaporate to dryness to obtain 12.5 mg of product, and the yield of soy isoflavone aglycone is 85%.

[0036] To analyze the obtained products, the method is as follows:

[0037] 1. Prepare 6 standard solutions of daidzein, daidzein, genistein, genistein, glycitein, and glycitin into 6 standard solutions of 1 mg / mL.

[0038] 2. The product prepared in this embodiment was dissolved in 10 mL of ethyl acetate as a sample liquid.

[0039] 3. Using the acti...

Embodiment 2

[0046] Take the alkaline extraction and acid precipitation process to produce 4L of soy milk water discharged from the soybean protein, and naturally settle for 5 hours to obtain 1000 mL of yellow liquid in the lower layer (the supernatant is colorless). Add 10g of activated clay to the liquid, magnetically stir for 1.5h, and filter to obtain activated clay residue. After natural drying or drying (<140°C), add 20mL of ethyl acetate, magnetically stir for 30min to extract, and centrifuge at 6000r / min for 15min. Take the ethyl acetate supernatant and spin-evaporate to dryness to obtain 12 mg of the product. The yield of soybean isoflavone aglycone is 85%.

[0047] After testing, the detection method is as in Example 1. The 12 mg product contains only daidzein, genistein, and glycitein, and no soy isoflavone glycosides. Among them, daidzein is 1.7 mg, genistein is 0.5 mg, and soy yellow Vegetarian 9.8mg.

Embodiment 3

[0049] Take the alkaline extraction and acid precipitation process to produce 4L of soy milk water discharged from the soybean protein, and naturally settle for 5 hours to obtain 1000 mL of yellow liquid in the lower layer (the supernatant is colorless). Add 12g of activated clay to the liquid, magnetically stir for 1h, and filter to obtain activated clay residue. After natural drying or drying (<140℃), add 40 mL of ethyl acetate, magnetically stir for 45 minutes, and centrifuge at 4000r / min for 10 minutes. The ethyl acetate supernatant was evaporated to dryness to obtain 13 mg of product, and the yield of soybean isoflavones was 83%.

[0050] After testing, the detection method is as in Example 1. The 13 mg product contains only daidzein, genistein, and glycitein, and no soy isoflavone glycosides. Among them, daidzein is 2.2 mg, genistein 0.6 mg, and soybean yellow Vegetarian 10.2mg.

[0051] The yield of soybean isoflavone aglycone prepared in Examples 1 to 3 is shown in Table 1...

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Abstract

The invention provides a method for extracting soy isoflavone aglycone from soybean milk water. The soybean milk water is used as a raw material solution, activated hargil is added into the raw material solution for adsorption, activated hargil residues are obtained after adsorption, ethyl acetate is used for extracting the activated hargil residues to obtain soy isoflavone aglycone, and the soybean milk water is soybean milk water discharged by an alkali extraction and acid precipitation soy protein process. According to the method disclosed by the invention, soy isoflavone glycoside does notneed to be used as a raw material, chemical reaction and biodegradation are not needed, the preparation method is simple, and the purity of the prepared soy isoflavone aglycone is relatively high.

Description

Technical field [0001] The present disclosure relates to a method for extracting soybean isoflavone aglycone from soybean milk water. Background technique [0002] The statements here only provide background information related to the present disclosure, and do not necessarily constitute prior art. [0003] Soybean is one of the important food crops in China, and it is a crop whose seeds are rich in plant protein. Soybeans are rich in isoflavones. Many studies in recent years have shown that soybean isoflavones have a variety of physiological functions, such as anti-cancer, anti-cardiovascular disease, anti-osteoporosis, anti-female menopausal syndrome, anti-senile dementia, anti-body immunity, anti-bacterial and anti-inflammatory, Anti-aging etc. [0004] Soy isoflavones mainly include: (1) trihydroxyisoflavones (also known as genistein, genistein), (2) dihydroxyisoflavones (also known as daidzein, daidzein, daidzein), (3) ) 12 kinds of compounds such as glycitein exist in natur...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/36C07D311/40
CPCC07D311/36C07D311/40
Inventor 刘汝萃李成辉崔玉涛侯尔群鲁绪强时玉强刘代成王笛
Owner SHANDONG YUWANG ECOLOGY FOOD IND
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