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Synthetic medium as well as preparation method and use thereof

A technology for synthesizing culture medium and glucose, which is applied in the field of fermentation, can solve the problem of low synthetic content of products, and achieve the effects of simple preparation, increased yield, and simple preparation method

Active Publication Date: 2019-12-10
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to solve the problem that the synthetic content of the product of gentamicin C1a fermentation in the above-mentioned prior art is very low, the present invention provides a method suitable for cultivating Micromonas crimsonosa or Micromonas aculeatus to efficiently produce gentamicin C1a. Synthetic medium formula of damycin C1a

Method used

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  • Synthetic medium as well as preparation method and use thereof
  • Synthetic medium as well as preparation method and use thereof
  • Synthetic medium as well as preparation method and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] This specific embodiment provides a kind of synthetic culture medium, and its composition and mass concentration thereof are as follows:

[0032] Glucose·H 2 O 28g / L, xylose 0.5g / L, ammonium sulfate 3g / L, sodium nitrate 2.3g / L, potassium chloride 0.5g / L, potassium dihydrogen phosphate 2.2g / L, ferrous sulfate 0.05g / L, Magnesium sulfate 0.045g / L, cobalt chloride 0.012g / L, calcium carbonate 4g / L, phenylalanine 0.05g / L, vitamin VB120.006g / L, vitamin VB30.0055g / L, biotin 0.015g / L , betaine 0.05g / L, and the remaining balance is water.

[0033] The preparation method of the synthetic medium is as follows:

[0034] Add xylose, ammonium sulfate, sodium nitrate, potassium chloride, potassium dihydrogen phosphate, ferrous sulfate, magnesium sulfate, cobalt chloride, phenylalanine, vitamin VB12, vitamin VB3, biotin, betaine into water and mix , adjust the pH value of the solution to 7.2-7.5;

[0035] Add calcium carbonate to the above solution and mix well, then sterilize at 12...

Embodiment 2

[0069] This specific example provides another embodiment of a kind of synthetic medium, and its composition and mass concentration thereof are as follows:

[0070] Glucose·H 2 O 10g / L, xylose 0.2g / L, ammonium sulfate 3g / L, sodium nitrate 2g / L, potassium chloride 0.2g / L, potassium dihydrogen phosphate 1g / L, ferrous sulfate 0.02g / L, magnesium sulfate 0.03g / L, cobalt chloride 0.005g / L, calcium carbonate 2g / L, phenylalanine 0.01g / L, vitamin VB120.001g / L, vitamin VB30.001g / L, biotin 0.001g / L, sugar beet Alkali 0.01g / L, the remaining balance is water.

[0071] The preparation method of the synthetic medium is as follows:

[0072] Add xylose, ammonium sulfate, sodium nitrate, potassium chloride, potassium dihydrogen phosphate, ferrous sulfate, magnesium sulfate, cobalt chloride, phenylalanine, vitamin VB12, vitamin VB3, biotin, betaine into water and mix , adjust the pH value of the solution to 7.2-7.5;

[0073] Add calcium carbonate to the above solution and mix well, then steri...

Embodiment 3

[0078] This specific example provides another embodiment of a synthetic culture medium, its composition and mass concentration are as follows:

[0079] Glucose·H 2 O 30g / L, xylose 1g / L, ammonium sulfate 10g / L, sodium nitrate 5g / L, potassium chloride 0.8g / L, potassium dihydrogen phosphate 3g / L, ferrous sulfate 0.08g / L, magnesium sulfate 0.06 g / L, cobalt chloride 0.02g / L, calcium carbonate 10g / L, phenylalanine 0.05g / L, vitamin VB120.01g / L, vitamin VB30.02g / L, biotin 0.03g / L, betaine 0.1g / L, the remaining balance is water.

[0080] The preparation method of the synthetic medium is as follows:

[0081]Add xylose, ammonium sulfate, sodium nitrate, potassium chloride, potassium dihydrogen phosphate, ferrous sulfate, magnesium sulfate, cobalt chloride, phenylalanine, vitamin VB12, vitamin VB3, biotin, betaine into water and mix , adjust the pH value of the solution to 7.2-7.5;

[0082] Add calcium carbonate to the above solution and mix well, then sterilize at 121°C for 25 minute...

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Abstract

The invention discloses a synthetic medium as well as a preparation method and a use thereof. The synthetic medium is characterized by being prepared from the following components by mass concentration: 10-30g / L of glucose.H2O, 0.2-1.0g / L of xylose, 3-10g / L of ammonium sulfate, 2-5g / L of sodium nitrate, 0.2-0.8g / L of potassium chloride, 1.0-3.0g / L of potassium dihydrogen phosphate, 0.02-0.08g / L offerrous sulfate, 0.03-0.06g / L of magnesium sulfate, 0.005-0.02g / L of cobalt chloride, 2-10g / L of calcium carbonate, 0.01-0.05g / L of phenylalanine, 0.001-0.01g / L of vitamin VB12, 0.001-0.02g / L of vitamin VB3, 0. 001-0.03g / L of biotin, 0.01-0.1g / L of betaine and the balance of water. The novel culture medium disclosed by the invention is used for culturing micromonospra and has an important significance of mechanism study on synthesizing secondary metabolites with fermentation of the micromonospra and industrial production of gentamicin C1a.

Description

technical field [0001] The invention belongs to the technical field of fermentation, and in particular relates to a synthetic medium and its preparation method and application. Background technique [0002] Gentamicin C1a is the mother core precursor of the semi-synthetic new drug ictimicin, which is the first in China and has independent intellectual property rights. The traditional method of preparing C1a is to use resin separation technology to extract C1a single component from multi-component gentamycin. Due to the similar structure and similar physical and chemical properties of each component, the extraction cost remains high, and the high cost in the market The price of purity C1a remains high. As one of the multi-components of gentamicin, gentamicin C1a uses a large number of organic nitrogen sources, carbon sources and other compounds in the industrial fermentation process, which leads to the inability to accurately determine the relationship between its product sy...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P19/48C12R1/01
CPCC12N1/20C12P19/485
Inventor 黄明志陈腾飞李敏超
Owner EAST CHINA UNIV OF SCI & TECH
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