Method and kit for rapidly detecting immunosuppressive agent in whole blood and application of kit

Abstract

The invention discloses a method and a kit for rapidly detecting an immunosuppressive agent in whole blood and application of the kit. The method for rapidly detecting the immunosuppressants in the whole blood comprises the following steps: carrying out sample treatment on a whole blood sample to be detected, and then carrying out liquid chromatography-tandem mass spectrometry detection, so that five immunosuppressants are detected at the same time; the sample treatment comprises the following steps: adding a precipitant 1 into a whole blood sample to be detected to carry out first precipitation treatment, adding a precipitant 2 to carry out second precipitation treatment, and centrifuging to obtain a supernatant; wherein the precipitant 1 is a zinc sulfate solution, the precipitant 2 is amethanol acetonitrile solution containing known amounts of internal standard substances, and the internal standard substances comprise a cyclosporine A isotope internal standard substance, an everolimus isotope internal standard substance, a sirolimus isotope internal standard substance and a mycophenolic acid isotope internal standard substance. According to the method and the kit disclosed by the invention, five immunosuppressants which are commonly involved and used can be detected at one time by only needing a small amount of whole blood samples, the method is simple and easy to operate,and a reference basis is provided for formulating an individualized dosing scheme.

Description

technical field [0001] The present application relates to the technical field of detection of immunosuppressants, in particular to a method, kit and application for rapid detection of immunosuppressants in whole blood. Background technique [0002] Immunosuppressants are a class of chemical or biological substances that reduce tissue damage by inhibiting cellular and humoral immune responses. They can inhibit abnormal immune responses in the body and are mainly used in the treatment of organ transplantation anti-rejection and autoimmune diseases. Since the development of immunosuppressants in the 1970s, the immunosuppressants currently being clinically used can be basically divided into five categories: the first category is cytokine inhibitors, such as cyclosporine A, tacrolimus, and serotonin. Limus, everolimus, etc.; the second category is DNA synthesis inhibitors, such as azathioprine, mycophenolic acid, mizoribine, etc.; the third category is glucocorticoids, such as pr...

AI Technical Summary

Problems solved by technology

[0004] Therefore, in general, the existing methods for detecting multiple immunosuppressants in whole blood can only effectively detect the four immunosuppressants CsA, Tac, Sir, and Ever, and cannot simultaneously detect CsA, Tac, Sir, Ever, and MPA. The method of five immunosuppressants cannot monitor the therapeutic drugs of the five immunosuppressants that are often used at the same time, and it is difficult to meet the needs of clinical use

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