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Micro-electrophoresis chip and method for online concentration and detection of charged small particles

A small particle and micro-electrophoresis technology, applied in the preparation of test samples, measuring devices, sampling, etc., can solve the problems of increasing the cost of the instrument and reducing the convenience of the instrument, so as to reduce the cost of the instrument, improve the portability and maximize the application potential Effect

Active Publication Date: 2019-12-13
NANJING UNIV OF INFORMATION SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The pneumatic valve concentration technology is very ingenious and can be integrated into the microchip, which is well matched with the microchip electrophoresis technology; however, this method additionally introduces a high-precision pneumatic pump, which realizes the closing and closing of the pneumatic valve by accurately injecting and sucking out trace gas. open, which greatly reduces the convenience of the instrument and increases the cost of the instrument

Method used

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  • Micro-electrophoresis chip and method for online concentration and detection of charged small particles
  • Micro-electrophoresis chip and method for online concentration and detection of charged small particles
  • Micro-electrophoresis chip and method for online concentration and detection of charged small particles

Examples

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Embodiment 1

[0060] Such as figure 2 , image 3 As shown, a microelectrophoresis chip for online concentration and detection of small particles includes a substrate with three channels in the substrate, and five pools on the substrate, namely: sample pool P, concentration pool A, and sample waste pool B. Buffer pool C, buffer waste pool D. It also includes five high-voltage electrodes and a pair of detection electrodes (respectively the detection signal transmitting electrode 12 and the detection signal receiving electrode 13).

[0061] The concentration channel PA connects the sample pool P and the concentration pool A;

[0062] Such as Figure 4 As shown, the concentration channel PA 14 is connected to the sample cell P and the concentration cell A;

[0063] Such as Figure 5 As shown, the sample injection channel AB 15 connects the concentration tank A and the sample waste liquid tank B;

[0064] Such as Image 6 As shown, the separation channel CD 16 connects the buffer pool C and the buffer...

Embodiment 2

[0070] Optionally, in this embodiment, there are three sample cells P, which are connected in parallel with the concentration cell A, and there are three sample cells.

Embodiment 3

[0072] Optionally, in this embodiment, the concentration tank A is provided with an adjusting plunger for adjusting the volume, and the volume adjustment range is 100 nanoliters to 2500 nanoliters;

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Abstract

The invention provides a micro-electrophoresis chip and method for online concentration and detection of charged small particles. The micro-electrophoresis chip comprises a substrate, three channels are arranged in the substrate, a plurality of pools are arranged on the substrate; the concentration channel PA is communicated with the sample pool P and the concentration pool A, the sample injectionchannel AB is communicated with the concentration pool A and the sample waste liquid pool B, the separation channel CD is communicated with the buffer solution pool C and the buffer solution waste liquid pool D, and the separation channel CD is vertically communicated with the sample injection channel AB in a crossed manner; high-voltage electrodes are arranged in the five pools respectively andare used for applying high voltage into the pools; the volume of the concentration pool A is adjustable, and the volume of the sample pool P is 10-1000 times that of the concentration pool A; and a pair of detection electrodes is arranged in a position close to the buffer solution waste liquid pool D of the separation channel CD and is used for detecting charged small particles passing the position. According to the technology, the detection precision of an original microchip electrophoresis and capacitive coupled non-contact conductivity detection method can be greatly improved; and the micro-electrophoresis chip and method also have the advantages of low-cost, easy carrying, rapid detection and in-situ detection of ME-C4D.

Description

Technical field: [0001] The invention relates to a concentration and detection technology of charged small particles, belonging to the field of biochemical analysis instruments and technologies. Background technique: [0002] Charged small particles usually include inorganic small ions such as nutrient segregants and heavy metal ions in the soil and water environment, as well as various inorganic small ions in the human body, animals and plants, DNA, protein and other charged small particles. Others can be added through charge addition technology (such as magnetic bead adsorption) ) The small charged particles produced also belong to the above category. For the processing and detection technologies of these charged small particles, currently available methods include spectroscopy, mass spectrometry, chromatography, electrochemical analysis, etc. The above method has the disadvantages of expensive equipment, complicated operation, single function, not easy to portability, and low...

Claims

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Application Information

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IPC IPC(8): G01N1/40G01N27/06
CPCG01N1/40G01N27/06G01N2001/4038
Inventor 杨明鹏尤晖宋苗苗黄哲
Owner NANJING UNIV OF INFORMATION SCI & TECH
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