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Substrates with modified surface reactivity and antifouling properties in biological reactions

A substrate and oxide technology, applied in biochemical equipment and methods, bioreactor/fermenter combination, specific-purpose bioreactor/fermenter, etc., can solve the problem of reducing the accuracy of results

Pending Publication Date: 2019-12-17
QUANTUM SI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Where a population of non-specifically bound molecules is within such a region, filtering out interfering signals during analysis can be challenging and can reduce the accuracy of results by facilitating overall statistical analysis

Method used

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  • Substrates with modified surface reactivity and antifouling properties in biological reactions
  • Substrates with modified surface reactivity and antifouling properties in biological reactions
  • Substrates with modified surface reactivity and antifouling properties in biological reactions

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0125] Example 1. Improved wettability on metal oxide surfaces using block copolymers

[0126] Methods for passivating metal oxide surfaces were evaluated by wettability experiments. with TiO 2 and SiO 2 Samples of the constructed surface area were subjected to a two-step process involving the formation of the coating followed by the formation of a stain-resistant cover. For the purposes of these experiments, hexylphosphonic acid (HPA) was used to generate the coating, and Pluronic P123 triblock copolymer (HO(C 2 h 4 O) a (C 3 h 6 O) b (C 2 h 4 O) a H) Creating an antifouling layer.

[0127] make TiO 2 / SiO 2 The samples were exposed to HPA over a period of 24 hours to allow the HPA monolayer to 2 self-assembled on the surface. After HPA passivation, the surface wettability was evaluated by contact angle measurement, where the TiO 2 The drop on the side measures about 85° and the SiO 2 The droplet measures about 15° on the side. The curve of contact angle v...

Embodiment 2

[0129] Example 2. Binding Selectivity Assay for Assessing Surface Functionalization

[0130] in TiO 2 / SiO 2 On samples, the surface selectivity of silanization was assessed by quantum dot (QD)-streptavidin analysis using biotin-conjugated silanes. In these experiments, the surface selectivity of silane compounds was examined for samples prepared with HPA passivated coating only, or samples prepared by HPA passivation followed by stain-resistant coverage of triblock copolymers. Fluorescence imaging results for the different conditions analyzed are shown in Figure 7 middle.

[0131] Using TiO that has not been subjected to a surface chemical reaction modification step 2 / SiO 2 The sample obtained the background fluorescence measurement value ( Figure 7 ,top). For control experiments, TiO was prepared by passivation with HPA followed by surface functionalization with silane-PEG-biotin 2 / SiO 2 sample. Fluorescence measurements were obtained using the QD-streptavidin ...

Embodiment 3

[0133] Example 3. Antifouling effect in biological reaction

[0134] The effect of stain-resistant coverage on the sequencing chip was assessed by observing the fluorescence emission from the dye-labeled nucleotides on the chip treated with HPA or HPA / triblock copolymer. like Figure 8 As shown in (left), minimal fluorescent signal was observed in chips treated with HPA / triblock copolymer. In contrast, chips treated with HPA alone exhibited a much higher emission detected from labeled nucleotides in solution ( Figure 8 ,right). These results indicate that HPA-treated chips are more susceptible to dye-labeled nucleotides remaining in the evanescent volume of the sample well than HPA / triblock copolymer-treated chips. Additionally, this would suggest that the triblock copolymer imparts antifouling properties to minimize or eliminate the susceptibility of dyes to adhere to surfaces within the evanescent volume. The sequencing reaction was further performed using chips treated...

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Abstract

The present invention provides a method of preparing the surface of a sample well. In some aspects, methods of preparing a sample well surface involve contacting the sample well with a block copolymer to form a stain-resistant coating over the metal oxide surface of the sample well. In some aspects, methods of passivating and / or selectively functionalizing a surface of a sample well are provided.

Description

[0001] related application [0002] Pursuant to 35 U.S.C. §119(e), this application claims priority to U.S. Provisional Patent Application No. 62 / 502,525, filed May 5, 2017, which is hereby incorporated by reference in its entirety. technical field [0003] The present application is generally directed to selectively modified substrate surface moieties and methods of making the same. Aspects of the technology provided herein involve altering the reactivity of one or more desired portions of a sample well surface. In some aspects, the present disclosure provides methods of selectively modifying one or more surface portions of a sample well. Background technique [0004] Microarrays are widely used as tools in single-molecule analysis, including nucleic acid analysis, nucleic acid sequencing, gene expression analysis, genotyping, mutation analysis, peptide analysis, peptide sequencing, and drug screening. Microarrays are typically formed on the surface of glass, metal, plast...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12M1/00C12M1/34
CPCB01L3/5085B01L2200/12B01L2300/16C09D5/1662C09D171/02B01L2300/0829B01L2300/0851B01L2300/0819C09D187/005B01L3/508C12Q1/6848B01L2200/141B01L2300/165
Inventor 乔纳森·M·罗斯伯格杰瑞米·拉基陈国钧
Owner QUANTUM SI
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