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Method for preparing glucose oxidase-modified metal organic framework pharmaceutical composition

A glucose oxidase and metal-organic framework technology, applied in the field of biomedicine, can solve the problems of tumor cell apoptosis and achieve huge economic and social benefits, enhanced anti-tumor effect, and low cost

Active Publication Date: 2019-12-20
ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In view of the above situation, in order to solve the defects of the prior art, the purpose of the present invention is to provide a method for preparing a glucose oxidase-modified metal-organic framework pharmaceutical composition, which can effectively solve the problem of triple-strike interactive comprehensive treatment for tumors, and promote tumor cell apoptosis

Method used

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Examples

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Embodiment 1

[0019] A method for preparing a glucose oxidase-modified metal organic framework pharmaceutical composition, comprising the following steps:

[0020] (1) Dissolve 285 mg of terephthalic acid into 7 mL of N,N-dimethylformamide solvent, weigh 935 mg of ammonium cerium nitrate and dissolve in 7 mL of ultrapure water to form ammonium cerium nitrate aqueous solution, Seal it, mix the N,N-dimethylformamide solution of terephthalic acid and the cerium ammonium nitrate aqueous solution, stir in an oil bath at 100°C for 17 minutes, and centrifuge at 12,500 rpm for 10 minutes to collect the precipitate, which is first washed with N,N- Wash 4 times with dimethylformamide to remove excess terephthalic acid, then wash 4 times with acetone to remove residual N,N-dimethylformamide, dry at 70°C for 24 hours, and grind to pass through a 110-mesh sieve The white powder of Ce-MOF is obtained;

[0021] (2) Calcining the Ce-MOF prepared in step (1) under the protection of nitrogen, raising the te...

Embodiment 2

[0025] A method for preparing a glucose oxidase-modified metal organic framework pharmaceutical composition, comprising the following steps:

[0026] (1) Dissolve 280 mg of terephthalic acid into 5 mL of N,N-dimethylformamide solvent, weigh 930 mg of ammonium cerium nitrate and dissolve in 5 mL of ultrapure water to form ammonium cerium nitrate aqueous solution, Seal it, mix the N,N-dimethylformamide solution of terephthalic acid and the cerium ammonium nitrate aqueous solution, stir in an oil bath at 90°C for 15 minutes, and centrifuge at 10,000 rpm for 10 minutes to collect the precipitate. The precipitate is first washed with N,N- Wash 3 times with dimethylformamide to remove excess terephthalic acid, then wash 3 times with acetone to remove residual N,N-dimethylformamide, dry at 70°C for 24 hours, and grind to pass through a 100-mesh sieve The white powder of Ce-MOF is obtained;

[0027] (2) Calcining the Ce-MOF prepared in step (1) under the protection of nitrogen, raisi...

Embodiment 3

[0031] A method for preparing a glucose oxidase-modified metal organic framework pharmaceutical composition, comprising the following steps:

[0032] (1) Dissolve 290mg of terephthalic acid into 10 mL of N,N-dimethylformamide solvent, weigh 940 mg of cerium ammonium nitrate and dissolve it in 10 mL of ultrapure water to form an aqueous solution of cerium ammonium nitrate, seal it, Mix the N,N-dimethylformamide solution of terephthalic acid with the cerium ammonium nitrate aqueous solution, stir in an oil bath at 110°C for 20 minutes, and centrifuge at 15,000 rpm for 10 minutes to collect the precipitate. Wash 5 times with formamide to remove excess terephthalic acid, then wash 5 times with acetone to remove residual N,N-dimethylformamide, dry at 70°C for 24 hours, and grind to a white powder passing through a 120-mesh sieve , to get Ce-MOF;

[0033] (2) Calcining the Ce-MOF prepared in step (1) under the protection of nitrogen, raising the temperature to 410°C at 6°C / min, cal...

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Abstract

The invention discloses a method for preparing a glucose oxidase-modified metal organic framework pharmaceutical composition, wherein the metal organic framework is cerium-based MOF (Ce-MOF). The method comprises the following steps: carbonizing synthesized Ce-MOF by calcination under protection of nitrogen, modifying glucose oxidase on a surface of the Ce-MOF through an amide bond reaction, and finally physically loading an anti-tumor drug inside the Ce-MOF to obtain the glucose oxidase-modified metal organic framework pharmaceutical composition with a particle size of 150-250 nm. The methodis stable and reliable, and low in cost. The prepared pharmaceutical composition can play a role of triple-strike interactive comprehensive treatment on tumors in preparation of anti-tumor drugs, enhance anti-tumor effect, is an innovation in the anti-tumor drugs, and has huge economic and social benefits.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a method for preparing a glucose oxidase-modified metal-organic framework pharmaceutical composition. Background technique [0002] In recent years, metal organic frameworks (Metal organic frameworks, MOF) have not only been used in traditional storage, separation and catalysis, but also have been intensively studied in biomedical applications, such as imaging and cancer treatment or as biosensor materials, MOF With the advantages of adjustable size, high surface area, and large internal pore volume, it can be an excellent drug carrier, and the surface has functional functional groups to facilitate the modification of macromolecules; among them, cerium-based MOF (Ce-MOF) contains peroxidase Active cerium oxide (CeO 2 ) nanoparticles, so it can interact with hydrogen peroxide (H 2 o 2 ) undergoes a Fenton-like reaction to generate reactive oxygen species (Reactive oxygen species, ROS...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/51A61K47/22A61K45/06A61K31/704A61K31/337A61K38/44A61P35/00
CPCA61K9/5123A61K45/06A61K31/704A61K31/337A61K38/443A61P35/00C12Y101/03004A61K2300/00
Inventor 冯倩华郝雨桐王宁王泽颖张雪莉张振中
Owner ZHENGZHOU UNIV
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