Treatment method for aniline wastewater
A technology for aniline wastewater and a treatment method, which is applied in the field of water treatment, can solve the problems of high requirements for wastewater components and high treatment costs, and achieves the effects of cost reduction and good operation stability.
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Embodiment 1
[0032] To prepare co-immobilized enzymes, proceed as follows:
[0033] Step 1: Prepare a mixed buffer solution of catalase and laccase.
[0034] Preparation of phosphate buffer:
[0035] Weigh Na 2 HPO 4 3.4092g, NaH 2 PO 4 1.5944g, dissolved in distilled water, adjusted to pH 7.0, and adjusted to 500mL to obtain 0.05M phosphate buffer, stored at 4°C (boiled before use).
[0036] Preparation of catalase solution:
[0037] Accurately weigh a certain quality of catalase freeze-dried powder (commercially available product) on an electronic balance, dissolve in the phosphate buffer saline solution of 0.05M with pH=7, obtain the enzyme solution of certain concentration, place 4 after preparing ℃ refrigerator and use as soon as possible.
[0038] Preparation of acetic acid-sodium acetate buffer:
[0039] Solution 1: 0.2mol / L sodium acetate solution, take 8.2g sodium acetate, dissolve it in deionized water and set the volume in a 500mL volumetric flask.
[0040] Solution 2...
Embodiment 2
[0047] Take the co-immobilized enzyme prepared in Example 1, put 4g of co-immobilized enzyme into a 250mL Erlenmeyer flask, add 100mL of aniline simulated wastewater, add H 2 o 2 As a pro-oxidant, the dosage is 0.3wt% of the aniline simulated wastewater, adjust the pH=5, place each group of Erlenmeyer flasks in a constant temperature shaker at 35°C and a speed of 140rpm, and shake the reaction. The total reaction time is 24h. After the end, the removal rate of aniline was measured as high as 73.26%.
Embodiment 3
[0049] Weigh the co-immobilized enzyme prepared in Example 1, put 6g of co-immobilized enzyme into a 250mL Erlenmeyer flask, add 100mL of aniline simulated wastewater, and place each group of Erlenmeyer flasks in a constant temperature shaker at 25°C and a speed of 140rpm , shaking reaction, the total reaction time is 24h, and the removal rate of aniline is as high as 71.8% after the reaction.
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