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A triterpene compound with anti-tumor activity isolated from discarded Trichosanthes fruit pulp and its application

A technology of triterpene compound and triterpene compound, which is applied to the triterpene compound with anti-tumor activity and its application field isolated from the discarded Trillium fruit pulp, can solve the problems of discarded fruit pulp and environmental pollution, and achieve strong anti-tumor The effect of tumor activity and convenient clinical administration

Active Publication Date: 2021-06-22
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Purpose of the invention: The purpose of this invention is to solve the problem of waste and pollute the environment of Trichosanthes or bilateral Trichosanthes fruit pulp, provide a new triterpenoid compound with strong anti-tumor activity, and provide raw materials for the development of anti-tumor drugs

Method used

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  • A triterpene compound with anti-tumor activity isolated from discarded Trichosanthes fruit pulp and its application
  • A triterpene compound with anti-tumor activity isolated from discarded Trichosanthes fruit pulp and its application
  • A triterpene compound with anti-tumor activity isolated from discarded Trichosanthes fruit pulp and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A new triterpene compound, which is prepared by the following method:

[0032] (1) Take 10 kg of fresh Trichosanthes fruit pulp, add an appropriate volume of 95% ethanol to make the alcohol concentration reach 80%, reflux extraction for 2 hours, extract twice, filter, combine the filtrates, concentrate under reduced pressure until no alcohol smell, and obtain Trichosanthes Concentrate of fruit pulp ethanol extract.

[0033] (2) Take the concentrated solution of the ethanol extract of Trichosanthes fruit pulp, and extract with petroleum ether and ethyl acetate in sequence respectively to obtain the petroleum ether fraction, the ethyl acetate fraction and the raffinate fraction.

[0034] (3) get the ethyl acetate extract of step (2) gained and concentrate under reduced pressure, obtain the medicinal extract 30g of ethyl acetate extraction part, get ethyl acetate part and silica gel by weight ratio 1:2 mix sample, then with 1:2 The proportion of the sample amount of 1 was...

Embodiment 2

[0041] Embodiment 2 antitumor experiment

[0042] Lung cancer cells (A549, H1299), colon cancer cells (SW480, HT29), pancreatic cancer cells (BxPC-3, PANC-1), prostate cancer cells (PC3, LNCaP) were used for in vitro anti-tumor experiments using the CCK-8 method. The test drug is the triterpenoid compound prepared in Example 1. The sample solvent dimethyl sulfoxide was used as a control. The experimental results in Table 2 show that the triterpene compound prepared by the present invention has a strong inhibitory effect on tumor cells. In particular, it has a particularly significant inhibitory effect on lung cancer cells.

[0043] Table 2 The inhibitory effect of triterpenoids on tumor cells

[0044]

[0045]The above experimental results show that the present invention turns waste into treasure, and by a preferred method, using the fruit flesh of Trichomonas or bilateral Trichosanthes as raw material, a new triterpene cucurbitacin compound with better antitumor effect ...

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Abstract

The invention discloses a new triterpenoid compound isolated from discarded Trichosanthes or bilateral Trichosanthes fruit flesh, with molecular formula C 33 h 48 o 9 , in vitro anti-tumor activity studies show that the compound has anti-tumor activity, including strong anti-tumor effects on colon cancer cells, prostate cancer cells, pancreatic cancer cells, and lung cancer cells. The invention can solve the problem of discarding and polluting the environment of Trichosanthes or bilateral fruit pulp, and can provide raw materials for the development of antitumor drugs, and has important practical application value.

Description

technical field [0001] The invention relates to a compound with antitumor activity, in particular to a triterpene compound with antitumor activity extracted from the non-medicinal parts of Trichosanthes chinensis or the fruit flesh of Trichosanthes bilobata and its use in preventing and treating tumor diseases. Background technique [0002] Cucurbitaceae plant Trichosanthes kirilowii Maxim. or Trichosanthes rosthornii Harms is the base plant of Trichosanthes kirilowii Maxim. and Trichosanthes rosthornii Harms, which are listed in the current Chinese Pharmacopoeia. In recent years, the planting area of ​​Trichosanthes nationwide is about 500,000 mu, mainly concentrated in Anhui, Henan, Jiangsu and other places, to collect seeds and produce hanging melon seeds to generate economic benefits, and collect a small amount of trichosanthes peel and trichosanthes. The mass ratio of peel, seed and fruit flesh of Trichosanthes quinquefolius is 1:1:1 (dry weight). During the process of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07J9/00A61P35/00A61K31/575
CPCA61P35/00C07J9/00
Inventor 刘培张黄琴钱大玮赵明段金廒
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE