Selenium-enriched cordyceps militaris liquor with function of liver protection, and preparation method of selenium-enriched cordyceps militaris liquor
A Cordyceps militaris and selenium-enriched technology, which is applied in the preparation of alcoholic beverages, the function of food ingredients, animal feed, etc., can solve the problems of low selenium production rate, high impurities, and high cost of selenium-containing wine, so as to reduce liver damage and reduce Damage, easily absorbed effect
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[0039] The preparation method of the selenium-enriched Cordyceps militaris wine includes the following steps:
[0040] See CN201710433450.3 for the cultivation method of selenium-enriched Cordyceps militaris. The high selenium-tolerant Cordyceps militaris strain (Cordyceps militaris CP1, deposit number CCTCC NO:M 2019671) was cultivated in a medium supplemented with nano-selenium to obtain selenium-enriched Cordyceps militaris fruiting bodies with an organic selenium content of 120-140mg / kg.
[0041] The preparation method for preparing selenium-enriched Cordyceps militaris wine with selenium-enriched Cordyceps militaris fruit body is as follows ( figure 1 ):
[0042] (1) Preparation of selenium-enriched Cordyceps militaris extract:
[0043] 1) Processing the raw material of selenium-enriched Cordyceps militaris: Dry the dry sample of selenium-enriched Cordyceps militaris, crush it and sieve 200 meshes to obtain superfine selenium-enriched Cordyceps militaris fruit body powder, whic...
Embodiment 1
[0053] Example 1 Obtaining the raw material of selenium-enriched Cordyceps militaris
[0054] Using the selenium-enriched Cordyceps militaris cultivation method disclosed in CN201710433450.3, the highly selenium-tolerant Cordyceps militaris strain (Cordyceps militaris CP1, preservation number CCTCC NO:M 2019671) was cultured in a medium supplemented with nano-selenium to obtain organic selenium content Selenium Cordyceps militaris fruiting bodies at 120-140mg / kg. After harvesting the selenium-enriched Cordyceps militaris fruiting bodies obtained through biotransformation, they were dried at 45°C, added to the crushing device and pulverized, and passed through a 200-mesh sieve to obtain the selenium-enriched Cordyceps militaris fruiting body ultrafine bacteria powder ( figure 2 ) For subsequent extraction.
Embodiment 2
[0055] Example 2 Preparation of selenium-enriched Cordyceps militaris liquor in different alcohol concentrations
[0056] The selenium-enriched Cordyceps militaris fruit body supermicro bacteria powder obtained in Example 1 was immersed in six different pure grain wines with an alcohol content of 15-96 degrees at a material-to-liquid ratio of 2.5%, stirred and mixed, and ultrasonically extracted for 10 minutes, 15 Let stand for 9h at -20℃, filter and clarify to obtain the supernatant stock solution, repeat 3 times; the supernatant obtained by combining is the leaching stock solution ( image 3 ). 15°C, 25°C, 40°C, 46°C and 68°C wine can dissolve total selenium 482.9±15.6μg / L, 420.1±32.1μg / L, 319.9±10.5μg / L, 304.1±6.2μg / L, 305.6±16.8μg / L, while the total selenium dissolution of the 96-degree pure grain wine under this condition is only 104.1±9.5μg / L ( Figure 4 ). The selenium-enriched Cordyceps militaris fruiting bodies are rich in carotenoids. The selenium-enriched Cordyceps m...
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