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Bacterial strain for removing formaldehyde, formaldehyde scavenger and preparation method thereof

A formaldehyde scavenger and formaldehyde-removing technology, applied in the field of formaldehyde scavenger and its preparation, formaldehyde-removing bacterial strains, can solve the problems of poor formaldehyde removal effect, achieve excellent removal effect and strong formaldehyde degradation ability

Active Publication Date: 2021-05-25
北京森海氧源科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] 2. Urea-formaldehyde resin inevitably leaves a part of free formaldehyde in the glue-making process and emits to the outside world;
[0006] 3. During the curing process of wood-based panels, a part of the linear resin does not form a network structure and decomposes into free formaldehyde and emits to the outside;
[0012] The formaldehyde scavenger in the prior art still has the problem of poor formaldehyde scavenging effect

Method used

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  • Bacterial strain for removing formaldehyde, formaldehyde scavenger and preparation method thereof
  • Bacterial strain for removing formaldehyde, formaldehyde scavenger and preparation method thereof
  • Bacterial strain for removing formaldehyde, formaldehyde scavenger and preparation method thereof

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preparation example Construction

[0030] A preparation method of formaldehyde scavenger, comprising the following steps:

[0031] (1) activate the above-mentioned bacterial strain for removing formaldehyde, and then place the activated bacterial strain in a microbial culture medium containing formaldehyde to cultivate;

[0032] (2) Centrifuge the obtained bacterial suspension, remove the supernatant and resuspend the obtained bacterial liquid, then nitrogen freeze the obtained bacterial liquid, grind and rupture the nitrogen frozen bacterial liquid, and then Resuspension and centrifugation are carried out, and the supernatant obtained by centrifugation is mixed with trehalose, organic silicon and ferric sulfate to obtain the formaldehyde scavenger.

[0033] As can be seen from the foregoing description, the beneficial effects of the present invention are:

[0034] The bacterial strain used for removing formaldehyde of the present application has the characteristic of extremely strong ability to degrade formal...

Embodiment 1

[0041] The preparation method of the formaldehyde scavenger of the present embodiment may further comprise the steps:

[0042] (1) The mutagenesis of Bacillus subtilis was carried out using a normal temperature and pressure plasma mutagenesis machine (ARTP), and the formaldehyde culture solution with different concentrations was used as a screening. After diluting the bacterial suspension 100 times, draw 10ul drops on the ARTP circular receiving piece under aseptic conditions and spread evenly, and then place the receiving piece under the radiation emitter. Using helium as the working gas, instrument parameters: helium gas flow rate 10L / min, input power 120W, mutagenesis time set to 30s, 60s, 90s, 120s, 150s, 180s, 240s. Place the treated samples in EP tubes containing 1ml of normal saline, shake them on a vortex shaker for 2 minutes, so that the bacteria fall off the slide, and inoculate 100ul of them in beef extract peptone medium containing 100mg / L formaldehyde Perform sha...

Embodiment 2

[0047] The preparation method of the formaldehyde scavenger of the present embodiment may further comprise the steps:

[0048] (1) The mutagenesis of Bacillus subtilis was carried out using a normal temperature and pressure plasma mutagenesis machine (ARTP), and the formaldehyde culture solution with different concentrations was used as a screening. After diluting the bacterial suspension 100 times, draw 10ul drops on the ARTP circular receiving piece under aseptic conditions and spread evenly, and then place the receiving piece under the radiation emitter. Using helium as the working gas, instrument parameters: helium gas flow rate 10L / min, input power 120W, mutagenesis time set to 30s, 60s, 90s, 120s, 150s, 180s, 240s. Place the treated samples in EP tubes containing 1ml of normal saline, shake them on a vortex shaker for 2 minutes, so that the bacteria fall off the slide, and inoculate 100ul of them in beef extract peptone medium containing 100mg / L formaldehyde Perform sha...

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Abstract

The invention relates to a bacterial strain for removing formaldehyde, and the registration number of the bacterial strain in the General Microorganism Center of China Microorganism Culture Collection Management Committee is CGMCC NO.18343. The present invention also relates to a formaldehyde scavenger comprising the above-mentioned strain for scavenging formaldehyde and a preparation method thereof. The bacterial strain for removing formaldehyde, the formaldehyde scavenger and the preparation method thereof of the present invention have the advantages of rapidly and completely degrading formaldehyde, especially removing residual formaldehyde inside the wood-based panel, with excellent removal effect and no rebound.

Description

technical field [0001] The invention relates to formaldehyde removal technology, in particular to a bacterial strain for removing formaldehyde, a formaldehyde scavenger and a preparation method thereof. Background technique [0002] According to the "Toxic and Harmful Air Pollutants List (2018)" formulated by the Ministry of Ecology and Environment in conjunction with the Health and Health Commission, formaldehyde ranks second on the list of toxic and harmful air pollutants, and was identified as a Class 1 carcinogen as early as 2006. Wood-based panels are made of wood or other non-wood plants, separated into various unit materials by certain mechanical processing, and then glued together with adhesives. Formaldehyde is a raw material for the manufacture of synthetic resins, paints, plastics and man-made fibers, and is also an important raw material for the manufacture of urea-formaldehyde resin glue, melamine resin glue and phenolic resin glue in the wood-based panel indust...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20B01D53/84B01D53/72C12R1/125
CPCB01D53/72B01D53/84B01D2251/95B01D2257/708C12N1/20C12N1/205C12R2001/125Y02A50/20
Inventor 陈云平陈为健黄平左汉荣
Owner 北京森海氧源科技有限公司
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