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Mutant of xylanase T-Xyn and application of mutant

A technology of xylanase and mutant, applied in the field of enzyme engineering

Active Publication Date: 2019-12-20
BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Currently, only a few reports have investigated the hydrolyzate properties and XOS production of T. thermophila xylanase

Method used

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  • Mutant of xylanase T-Xyn and application of mutant
  • Mutant of xylanase T-Xyn and application of mutant
  • Mutant of xylanase T-Xyn and application of mutant

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Embodiment Construction

[0028] The present invention will be further elaborated below through specific embodiments or examples, so as to better understand the present invention.

[0029] 1. Materials and methods

[0030] 1. Genes, strains, media, vectors and reagents

[0031] Using T-Xyn from Talaromycesthermophilus F1208 (Q.Li, B.G.Sun, K.Xiong, C.Teng, Y.Q.Xu, L.J.Li, X.T.Li, Improving special hydrolysis characterization into Talaromycesthermophilus F1208 xylanase by engineering of N- terminal extension and site-directed mutagenesis in C-terminal, Int. J.Biol.Macromol.96(2017)451-458.), the recombinant pET-28a-Ttxyn expression vector was constructed.

[0032] Corn cobs (27.0% hemicellulose content) were purchased from Shandong Province, China and kept in a dry place; Escherichia coli (DH3α), Escherichia coli BL21(DE3), plasmids pMD18-T and pET28a(+), Taq polymerase, DNA Gel extraction kit, isopropyl-β-D-thiogalactopyranoside (IPTG), ampicillin, and kanamycin were purchased from Takara (Tokyo, Jap...

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Abstract

The invention provides a mutant T-XynT(38)S(50)C(122)C(166) of xylanase T-Xyn. The mutant has good heat resistance and hydrolyzing performance, and is high in yield of xylo-oligosaccharide generated from corn cobs, the reaction time is short to 2 hours, and far shorter than known enzymolysis reaction time, so that the mutant can be used for producing Xylo-oligosaccharides from agricultural wastes,and has great application potential.

Description

technical field [0001] The invention belongs to the field of enzyme engineering, in particular to the mutation of xylanase and its application in producing xylooligosaccharides from agricultural waste. Background technique [0002] The large amount of agricultural waste generated globally annually from the production and processing of agricultural products is a major component of lignocellulosic biomass. This lignocellulosic biomass is an underutilized resource rich in cellulose (40–50wt.%), hemicellulose (20–40wt.%) and lignin (10–30wt.%), which are useful renewable resource. Cellulose, hemicellulose, and lignin form dense structural networks that prevent their efficient use, so most lignocellulosic biomass is discarded or destroyed through uncontrolled combustion. In addition, the depletion of fossil fuels and increasing environmental and health concerns promote the development of agricultural waste-based technologies to produce biochemicals (e.g. xylooligosaccharides, p...

Claims

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Application Information

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IPC IPC(8): C12N9/24C12N15/56C12P19/14C12P19/04C12P19/12C12P19/00
CPCC12N9/248C12P19/00C12P19/04C12P19/12C12P19/14
Inventor 范光森李秀婷吴秋华滕超杨然
Owner BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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