Formaldehyde detection method
A detection method and formaldehyde technology are applied in the detection field to achieve the effect of strong anti-interference and high sensitivity
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Embodiment 1
[0025] The molecular structure of the fluorescent chromogenic agent of the present embodiment is shown in formula I:
[0026]
[0027] The H NMR spectrum of the compound is shown in image 3 shown.
[0028] The preparation method of the fluorescent chromogenic agent of the present embodiment is to dissolve 120mg (0.21mmol) of the compound of the structure shown in formula II in 10mL of tetrahydrofuran, and then add 100mg (0.65mmol) of p-nitrobenzaldehyde, 100 μL ( 1.6mmol) of glacial acetic acid and 180mg (0.9mmol) of sodium triacetate borohydride were stirred at room temperature for 18 hours. Glacial acetic acid, as a co-solvent for p-nitrobenzaldehyde, is beneficial to promote the mixing of p-nitrobenzaldehyde and other substances. The reaction formula is as follows:
[0029]
[0030] After the reaction was completed, pour 50 mL of saturated sodium bicarbonate solution, extract 4 times with 30 mL of ethyl acetate, and dry the organic phase in vacuum. Wash with wate...
Embodiment 2
[0035] The preparation method of the fluorescent chromogen of the present embodiment is to dissolve 120mg (0.21mmol) of the compound of the structure shown in formula II in 10mL of tetrahydrofuran, and then add 100mg (0.65mmol) of p-nitrobenzaldehyde 180mg (0.9 mmol) of sodium triacetate borohydride, stirred at room temperature for 18 hours. After the reaction was completed, pour 50 mL of saturated sodium bicarbonate solution, extract 4 times with 30 mL of ethyl acetate, and dry the organic phase in vacuum. Wash with water, methanol, and anhydrous ether several times. Vacuum dry. The yield was 70%.
Embodiment 3
[0037] The preparation method of the fluorescent chromogen of the present embodiment is to dissolve 120mg (0.21mmol) of the compound of the structure shown in formula II in 10mL of tetrahydrofuran, and then add 50 (0.325mmol) of p-nitrobenzaldehyde, 100 μL ( 1.6mmol) of glacial acetic acid and 180mg (0.9mmol) of sodium triacetate borohydride were stirred at room temperature for 18 hours. After the reaction was completed, pour 50 mL of saturated sodium bicarbonate solution, extract 4 times with 30 mL of ethyl acetate, and dry the organic phase in vacuum. Wash with water, methanol, and anhydrous ether several times. Vacuum dry. The yield was 70%.
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