A kind of high performance liquid phase detection method of glycyl-d-glutamine
A glutamine and high-performance liquid phase technology, applied in the field of high-performance liquid chromatography detection, can solve the problems of difficult separation, long reaction time, cumbersome operation steps, etc.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0050] Chromatographic conditions: covalently bonded on the surface of silica gel-quinine (8R, 9S)-(1R, 2R)-cyclohexylsulfamic acid derivatives as a chromatographic column, mobile phase is formic acid-diethylamine-methanol ( 1.9:2.6:995.5), the detection wavelength is 248nm, the flow rate is 0.2ml / min, the column temperature is 20°C, and the injection volume is 2μl.
[0051]Preparation of the test solution: Accurately weigh an appropriate amount of glycyl-L-glutamine, dilute it with water to a solution containing 3mg of glycyl-L-glutamine per 1ml of water, as the test solution; Take appropriate amount of glycyl-L-glutamine and glycyl-D-glutamine, dilute with water so that every 1ml of water contains 3mg of glycyl-L-glutamine, glycyl-D-glutamine 4.5 μg of the mixed solution, as a mixed reference solution. Precisely measure 10 μl of the test solution, put it in a dry derivation tube (diameter 6 mm, height 50 mm), add 30 μl of buffer solution (Buffer1), vortex mix, add 60 μl of ...
Embodiment 2
[0054] Chromatographic conditions: covalently bonded on the surface of silica gel-quinine (8R, 9S)-(1R, 2R)-cyclohexylsulfamic acid derivatives as a chromatographic column, mobile phase is formic acid-diethylamine-methanol ( 1.9:2.6:995.5), the detection wavelength is 248nm, the flow rate is 0.2ml / min, the column temperature is 22°C, and the injection volume is 2μl.
[0055] Preparation of the test solution: Accurately weigh an appropriate amount of glycyl-L-glutamine, dilute it with water to a solution containing 3mg of glycyl-L-glutamine per 1ml of water, as the test solution; Take appropriate amount of glycyl-L-glutamine and glycyl-D-glutamine, dilute with water so that every 1ml of water contains 3mg of glycyl-L-glutamine, glycyl-D-glutamine 4.5 μg of the mixed solution, as a mixed reference solution. Precisely measure 10 μl of the test solution, put it in a dry derivation tube (diameter 6 mm, height 50 mm), add 30 μl of buffer solution (Buffer1), vortex mix, add 60 μl of...
Embodiment 3
[0058] Chromatographic conditions: covalently bonded on the surface of silica gel-quinine (8R, 9S)-(1R, 2R)-cyclohexylsulfamic acid derivatives as a chromatographic column, mobile phase is formic acid-diethylamine-methanol ( 1.9:2.6:995.5), the detection wavelength is 248nm, the flow rate is 0.22ml / min, the column temperature is 20°C, and the injection volume is 2μl.
[0059] Preparation of the test solution: Accurately weigh an appropriate amount of glycyl-L-glutamine, dilute it with water to a solution containing 3mg of glycyl-L-glutamine per 1ml of water, as the test solution; Take appropriate amount of glycyl-L-glutamine and glycyl-D-glutamine, dilute with water so that every 1ml of water contains 3mg of glycyl-L-glutamine, glycyl-D-glutamine 4.5 μg of the mixed solution, as a mixed reference solution. Precisely measure 10 μl of the test solution, put it in a dry derivation tube (diameter 6 mm, height 50 mm), add 30 μl of buffer solution (Buffer1), vortex mix, add 60 μl o...
PUM
Property | Measurement | Unit |
---|---|---|
separation | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com