A kind of high performance liquid phase detection method of glycyl-d-glutamine

Abstract

The invention discloses a high-efficiency liquid-phase detection method for glycyl-D-glutamine, comprising the following steps: preparing a test solution; preparing a mixed reference solution; deriving the test solution; and deriving the mixed reference solution; Chromatography: Precisely measure 2 μl of each sample derived from step (3) and step (4). After the chiral chromatographic column is well balanced, inject it into the liquid chromatograph, collect the chiral separation chromatogram, record the chromatogram for 60 minutes, and check seperate effect. The present invention realizes the problem of chiral separation of glycyl-L-glutamine and glycyl-D-glutamine, improves the test efficiency, and the stability of the chiral column is relatively good. Good separation effect, wide application range and good economic value.

Description

technical field

[0001] The invention belongs to the technical field of high performance liquid chromatography detection and relates to a high performance liquid phase detection method of glycyl-D-glutamine. Background technique

[0002] The presence of amino acids in the human body not only provides important raw materials for protein synthesis, but also provides a material basis for promoting growth, normal metabolism, and maintaining life. Amino acid drugs have low side effects, high drug efficacy, strong pertinence, and will not accumulate in the body to cause poisoning.

[0003] Glycyl-L-glutamine is often used to provide glutamine to promote protein synthesis. The amino acids that make up proteins are all L-type amino acids, and the drugs obtained through chemical synthesis are often enantiomers, not a single optical Isomers, in many cases, there are significant differences in the pharmacological activity, metabolic process, metabolic rate and toxicity of a pair of ena...

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