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Production method of water-soluble chitosan liposome adjuvant for veterinary use

A technology of water-soluble chitosan and liposome, which is applied in the direction of medical preparations containing active ingredients, pharmaceutical formulas, allergic diseases, etc., can solve the high risk of organic solvent residue, short maintenance time of high-level antibodies, thermodynamic Poor stability and other problems, to achieve excellent hemostatic effect, small particle size distribution range, small molecular weight effect

Inactive Publication Date: 2019-12-27
WUHAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The current commercially available veterinary liposome adjuvants have the following problems: large particle size, poor uniformity, poor stability, poor thermodynamic stability, easy to separate after long-term storage, high risk of organic solvent residue in the adjuvant, high Horizontal antibodies are maintained in the body for a short time

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] A preparation method of water-soluble chitosan liposome adjuvant for animals, comprising the steps of:

[0041] (1) Dissolve phospholipids and cholesterol in chloroform and mix to obtain mixed solution A;

[0042](2) According to the ratio of propolis: 95% ethanol = 1kg: 15L, dissolve propolis in 95% ethanol to prepare propolis ethanol solution;

[0043] (3) The mixed solution A obtained in step (1) is uniformly mixed with the propolis ethanol solution obtained in step (2) according to a volume ratio of 1:3 to obtain a mixed solution B;

[0044] (4) Transfer the mixed solution B obtained in step (3) to a rotary evaporator, perform vacuum rotary evaporation to remove the organic solvent, and form a lipid film on the inner wall of the rotary evaporator flask;

[0045] (5) Wash the lipid film on the inner wall of the rotary evaporator flask in step (4) with deionized water, and mix the solution to obtain a mixed solution C;

[0046] (6) Incubating the mixed solution C ob...

Embodiment 2

[0054] A preparation method of water-soluble chitosan liposome adjuvant for animals, comprising the steps of:

[0055] (1) Dissolve phospholipids and cholesterol in chloroform and mix to obtain mixed solution A;

[0056] (2) According to the ratio of propolis: 95% ethanol = 1kg: 25L, dissolve propolis in 95% ethanol to prepare propolis ethanol solution;

[0057] (3) The mixed solution A obtained in step (1) is uniformly mixed with the propolis ethanol solution obtained in step (2) according to a volume ratio of 1:2 to obtain a mixed solution B;

[0058] (4) Transfer the mixed solution B obtained in step (3) to a rotary evaporator, perform vacuum rotary evaporation to remove the organic solvent, and form a lipid film on the inner wall of the rotary evaporator flask;

[0059] (5) Wash the lipid film on the inner wall of the rotary evaporator flask in step (4) with deionized water, and mix the solution to obtain a mixed solution C;

[0060] (6) Incubating the mixed solution C o...

Embodiment 3

[0068] A preparation method of water-soluble chitosan liposome adjuvant for animals, comprising the steps of:

[0069] (1) Dissolve phospholipids and cholesterol in chloroform and mix to obtain mixed solution A;

[0070] (2) According to the ratio of propolis: 95% ethanol = 1kg: 20L, dissolve propolis in 95% ethanol to prepare propolis ethanol solution;

[0071] (3) The mixed solution A obtained in step (1) is uniformly mixed with the propolis ethanol solution obtained in step (2) according to a volume ratio of 1:1 to obtain a mixed solution B;

[0072] (4) Transfer the mixed solution B obtained in step (3) to a rotary evaporator, perform vacuum rotary evaporation to remove the organic solvent, and form a lipid film on the inner wall of the rotary evaporator flask;

[0073] (5) Wash the lipid film on the inner wall of the rotary evaporator flask in step (4) with deionized water, and mix the solution to obtain a mixed solution C;

[0074] (6) Incubating the mixed solution C o...

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PUM

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Abstract

The invention discloses a production method of a water-soluble chitosan liposome adjuvant for veterinary use. The adjuvant comprises phospholipid, cholesterol, propolis, chitosan hydrochloride and glutaraldehyde. The water-soluble chitosan liposome adjuvant, produced through the production method, for veterinary use is small in particle size and good in uniformity, has good thermal stability and good cytocompatibility, is high in storage stability, and is not stratified after being placed for a long term, the risk of a residual organic solvent in the adjuvant is greatly eliminated, and hold time of a high level of antibody in a body can be prolonged.

Description

technical field [0001] The invention belongs to the technical field of veterinary vaccine adjuvants, in particular to a preparation method of veterinary water-soluble chitosan liposome adjuvants. Background technique [0002] Immune adjuvants can assist immunogens to stimulate the body to produce an earlier, stronger and longer-lasting immune response. Adjuvants can be roughly divided into two categories. The first category is antigen carriers, such as emulsions and liposomes, which can be more effective The second type is immunostimulants, such as Toll-like receptors (TLR), aluminum hydroxide, saponins, and cytokines. With the development of biotechnology, some new adjuvants gradually appear with the needs of the times, among which chitosan and its derivatives have good biodegradability, low toxicity, immunostimulatory activity, sustained release and targeting , has become an excellent carrier for constructing vaccine immune adjuvants. [0003] Aluminum salt has been used...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/39A61P37/04
CPCA61K39/39A61P37/04A61K2039/55555A61K2039/55583A61K2039/55588
Inventor 王春维王雪辉祝爱侠夏廉臣陶冶万蒙陈懿琳
Owner WUHAN POLYTECHNIC UNIVERSITY