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Digital polymerase chain reaction (PCR) system

A digital and droplet technology, which is applied in the fields of biomedicine and disease detection, can solve the problems of small flux, slow droplet formation speed, complicated operation, etc., and achieve the effect of reducing the amount of oil phase and consumption

Pending Publication Date: 2019-12-31
SHANGHAI IND U TECH RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0009] In view of the shortcomings of the prior art described above, the purpose of the present invention is to provide a digital PCR system and a method for forming digital PCR droplets, which are used to solve the problems of slow droplet formation, small flux, complicated operation, and PCR in the prior art. The problem of low oil utilization

Method used

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  • Digital polymerase chain reaction (PCR) system
  • Digital polymerase chain reaction (PCR) system
  • Digital polymerase chain reaction (PCR) system

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Embodiment 1

[0106] The present invention provides a digital PCR system, please refer to Figure 1a to Figure 1g ,in Figure 1a Shown as a schematic diagram of the three-dimensional structure of the digital PCR system, Figure 1b is shown as a top view of the digital PCR system, Figure 1c is shown as a bottom view of the digital PCR system, Figure 1d , Figure 1e , Figure 1f and Figure 1g They are respectively shown as side views of the digital PCR system in four directions. figure 2 It is shown as a schematic diagram of an exploded structure of the digital PCR system. In this embodiment, the digital PCR system includes at least one droplet forming component 1 and a droplet orifice component 3, and the droplet orifice component 3 is connected to the Droplet forming assembly 1 below.

[0107] Specifically, the droplet forming assembly 1 includes at least one droplet collection tank 2 . Figure 1a Shown in is the case where the number of the droplet forming assembly 1 is one, and ...

Embodiment 2

[0139] The present invention also provides a digital PCR droplet forming method, comprising the following steps: vaporizing part of the digital PCR solution liquid by using a vaporization component and quickly pushing the rest of the digital PCR solution liquid into the droplet forming oil to form a digital PCR liquid drop.

[0140] As an example, a thermal bubble technique is used for high-speed digital PCR droplet formation, and the vaporization part includes a heating part for partially vaporizing the digital PCR solution liquid by heating it.

[0141] Specifically, the formation speed of the digital PCR droplets is controlled by controlling the heating time, heating frequency and heating interval time of the heating component. Using the digital PCR droplet forming method of the present invention can achieve a digital PCR droplet formation speed greater than 1000 / second.

[0142] As an example, the digital PCR droplet forming method includes the following steps:

[0143] ...

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Abstract

The invention provides a digital polymerase chain reaction (PCR) system. The digital PCR system comprises a droplet formation assembly and a droplet nozzle assembly; the droplet formation assembly comprises a droplet collection tank; the droplet nozzle assembly is connected under the droplet formation assembly, and comprises a plurality of droplet nozzles; the droplet nozzles communicate with thedroplet collection tank; vaporizing components are arranged inside the droplet nozzles so as to be used for vaporizing part of the liquid of a digital PCR solution in the droplet nozzles, as well as quickly pushing the remaining liquid of the digital PCR solution into droplet forming oil in the droplet collection tank so as to form digital PCR droplets. By adopting a thermal bubble technology to form high-speed digital PCR droplets, the digital PCR system is capable of achieving a droplet formation rate of more than 1000 droplets per second; and moreover, the digital PCR system has highly efficient digital PCR oil utilization rate.

Description

technical field [0001] The invention belongs to the field of biomedicine, in particular to the field of disease detection, and relates to a digital PCR system and a digital PCR droplet forming method. Background technique [0002] It has been 20 years since the polymerase chain reaction (PCR) was proposed. During this period, PCR has developed into a key technology and a routine technology in the field of molecular biology, which has greatly promoted the development of various fields of life science. Especially in the late 1990s, the real-time fluorescence quantitative PCR (real time PCR, qPCR) technology and related products launched by the American ABI company developed PCR from in vitro synthesis and qualitative / semi-quantitative detection technology into a highly sensitive and highly specific method. and precise quantitative genetic analysis techniques. [0003] Although after more than ten years of rapid development, qPCR technology has been used in the diagnosis of al...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/38C12M1/00C12Q1/686
CPCB01L7/52C12Q1/686C12Q2563/159
Inventor 吴炫烨关一民
Owner SHANGHAI IND U TECH RES INST
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