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Waterbody pathogen concentration apparatus and concentration method

A technology for concentrating devices and pathogens, applied in the field of microbial detection, can solve the problems of effective application range, large volume, difficult detection, etc., and achieves the effects of not affecting the complete collection of pathogens, simple device structure, and ensuring reliability.

Pending Publication Date: 2019-12-31
NINGBO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the large volume of the water body, the concentration of pathogens is relatively low, and most of them are attached to the particles in the water, which is more difficult to detect than clinical specimens. It is usually necessary to effectively enrich and concentrate the pathogens in the water body by chemical methods or special devices. make it detectable
Existing methods for concentrating pathogens in water mainly include: filtration, precipitation, adsorption-elution, ultrafiltration, reverse osmosis, dehydration dialysis, electrical barrier and immunological methods, etc. Although these concentration methods Each has its own advantages, but also has its own limitations, such as effective application range, limited treatment water volume, easy to cause pathogen inactivation, false negative test results, etc., and the existing methods often need to collect the water body to be tested, and then After being transported to the laboratory or laboratory, the concentration device is used for concentration. Due to the large volume of the water body, it is difficult to collect and carry the water body, which causes some inconvenience to the development of field water body testing.

Method used

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  • Waterbody pathogen concentration apparatus and concentration method
  • Waterbody pathogen concentration apparatus and concentration method
  • Waterbody pathogen concentration apparatus and concentration method

Examples

Experimental program
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Effect test

Embodiment 1

[0042] Example 1: A water body pathogen concentration device

[0043] refer to Figure 1-4, the present invention provides a water pathogen concentration device 1, said device 1 comprising: a water tank 10, a water pump 20, a pathogen concentrator 30 and a connecting pipe 40 for connecting the above components, wherein the outlet of the water tank The water port 102 is connected with the water inlet 303 of the pathogen concentrator, and the first water outlet 304 of the pathogen concentrator is connected with the water inlet 101 of the water tank, so the water pump 20 is located at the water outlet 102 of the water tank and between the pathogen concentrator. Between the water inlets 303. In this embodiment, the water body to be concentrated is injected into the water tank 10 through the water tank water inlet 101, and the water outlet 102 of the water tank is connected with the water pump 20, and the water pump 20 is used to pump the water in the water tank 10 into the path...

Embodiment 2

[0065] Embodiment 2: the concentrated detection of PMMoV plant virus in the water body

[0066] The concentration device is installed with a 500nm pore size inorganic ceramic ultrafiltration membrane. First, the water to be tested (50L raw water) is filtered through a 600-mesh stainless steel mesh to filter large particles such as duckweed and gravel, and the filtered water is slowly poured into the tank. First use the 500nm aperture inorganic ceramic ultrafiltration membrane (pressure 0.2MPa, temperature 10-40°C) to retain the filtrate (called 500nm filtrate). The 500nm filtrate was slowly injected into the tank again, and filtered with a 50nm pore size inorganic ceramic ultrafiltration membrane (pressure 0.2MPa, temperature 10-40°C). After the filtration is completed, take out the ultrafiltration membrane, and use a 50mL syringe to rinse with 20-50mL 1×PBS several times, and the final rinse solution is the concentrated solution. Draw 5 μL each of raw water, concentrated s...

Embodiment 3

[0067] Example 3: Bacterial Filtration

[0068] Install the concentration device with a 500nm pore size inorganic ceramic ultrafiltration membrane, first pass the water to be tested through a 600-mesh stainless steel mesh to filter large particles such as duckweed and gravel, and then slowly pour the filtered water into the tank. First use inorganic ceramic ultrafiltration membrane with a pore size of 500nm (pressure 0.2MPa, temperature 10-40°C) to retain the filtrate. Take 100 μL of raw water and 500nm filtrate and spread them on LB medium, and culture them at 28°C for 48 hours. The results show that the number of colonies in raw water is significantly higher than that in filtered water ( Figure 5 ), indicating that the device can retain most bacteria.

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Abstract

The present invention discloses a waterbody pathogen concentrating apparatus and a concentration method. The apparatus includes a water tank, a water pump, a pathogen concentrator, and a connecting pipeline for connecting the above components. A water outlet of the water tank is connected to a water inlet of the pathogen concentrator are connected, a first water outlet of the pathogen concentratoris connected to a water inlet of the water tank, and the water pump is located between the water outlet of the water tank and the water inlet of the pathogen concentrator. The pathogen concentrator includes a casing and an inorganic ceramic ultrafiltration membrane filter element arranged in the casing, and the pathogen concentrator further includes a second water outlet. The apparatus of the present invention has a simple structure, a small volume, and is easy to carry, and is particularly suitable for rapidly and continuously enriching pathogens in environmental water bodies in a field scene such as a farm field to make the pathogens detectable. The apparatus can ensure that the pathogens in the water bodies are concentrated or collected as much as possible, and ensures activity of thepathogens and reliability of test results.

Description

technical field [0001] The invention relates to the technical field of microorganism detection, in particular to a water body pathogen concentration device and a concentration method. Background technique [0002] Water resources are an important material basis for human survival and development. The quality of water environment is one of the most important and key barriers in the water and food safety system. Therefore, water environmental pollution has always been one of the focuses of public and environmental science research. one. The pollution of pathogens in water bodies is an important problem of water environment pollution. Pathogens in natural water bodies can survive for a long time and infect the human body through drinking water, diet or skin contact, endangering human production and human health. [0003] The detection of pathogens in water is a major technical indicator for evaluating water treatment, and it is also an important detection standard for agricult...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/40C02F1/44
CPCG01N1/4077C02F1/444G01N2001/4088
Inventor 彭杰军鲁宇文尹跃艳严丹侃郑红英燕飞林林陈剑平
Owner NINGBO UNIV
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