Preparation method of macadimia nut protein and application of preparation method

A technology for nut protein and protein extract, applied in the field of preparation of macadamia nut protein, can solve the problems of protein denaturation and oxidative deterioration of degreasing nut meal, and achieve the effects of preventing oxidation, preventing the generation of harmful substances and improving quality.

Pending Publication Date: 2020-01-07
JIANGCHENG ZHONGAO AGRI TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the extraction method of macadamia nut oil used in industry (such as hot pressing method, etc.) will cause the oil to oxidize and deteriorate, and even produce harmful substances such as trans fatty acids and oil polymers. Nut meal should only be used as animal feed

Method used

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  • Preparation method of macadimia nut protein and application of preparation method
  • Preparation method of macadimia nut protein and application of preparation method
  • Preparation method of macadimia nut protein and application of preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Embodiment 1: Example of preparation of macadamia nut protein

[0042] The extraction steps of macadamia nut protein are as follows:

[0043] Step (1): using macadamia nut kernels to prepare defatted nut meal, crushing and passing through a 40-mesh sieve to obtain defatted fruit meal powder;

[0044] Step (2): Disperse the defatted fruit meal powder in water, and the dosage ratio of the defatted fruit meal powder to water is 1g:6ml. Then add compound enzyme A, mix evenly to obtain enzymatic hydrolysis system A, and the mass concentration of compound enzyme A in enzymatic hydrolysis system A is 8%. Compound enzyme A is a mixture of cellulase (aladdin, C298989, CAS number: 9012-54-8) and amylase (aladdin, A109181, CAS number: 9000-90-2), the quality of cellulase and amylase The ratio is 1:1. The enzymatic hydrolysis reaction is carried out on the defatted fruit meal powder, the temperature of the enzymatic hydrolysis reaction on the defatted fruit meal powder is 40° C....

Embodiment 2

[0052] Embodiment 2: Example of preparation of macadamia nut protein

[0053] The extraction steps of macadamia nut protein are as follows:

[0054] Step (1): using macadamia nut kernels to prepare defatted nut meal, crushing and passing through a 40-mesh sieve to obtain defatted fruit meal powder;

[0055] Step (2): Disperse the defatted fruit meal powder in water, and the dosage ratio of the defatted fruit meal powder to water is 1g:5ml. Then add compound enzyme A, mix evenly to obtain enzymatic hydrolysis system A, and the mass concentration of compound enzyme A in enzymatic hydrolysis system A is 6%. Compound enzyme A is a mixture of cellulase and amylase, and the mass ratio of cellulase and amylase is 1:1. The enzymatic hydrolysis reaction is carried out on the defatted fruit meal powder, the temperature of the enzymatic hydrolysis reaction on the defatted fruit meal powder is 30°C, the time is 120min, and the pH value is 7.0.

[0056] Step (3): After the enzymolysis r...

Embodiment 3

[0062] Embodiment 3: Example of preparation of macadamia nut protein

[0063] The extraction steps of macadamia nut protein are as follows:

[0064] Step (1): using macadamia nut kernels to prepare defatted nut meal, crushing and passing through a 40-mesh sieve to obtain defatted fruit meal powder;

[0065] Step (2): Disperse the defatted fruit meal powder in water, and the dosage ratio of the defatted fruit meal powder to water is 1g:8ml. Then add compound enzyme A, mix evenly to obtain enzymatic hydrolysis system A, and the mass concentration of compound enzyme A in enzymatic hydrolysis system A is 9%. Compound enzyme A is a mixture of cellulase and amylase, and the mass ratio of cellulase and amylase is 1:1. The enzymatic hydrolysis reaction is carried out on the defatted fruit meal powder, the temperature of the enzymatic hydrolysis reaction on the defatted fruit meal powder is 30°C, the time is 120min, and the pH value is 7.0.

[0066] Step (3): After the enzymolysis r...

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Abstract

The invention belongs to the field of natural plant product extraction, relates to a preparation method of a macadimia nut protein and application of the preparation method, and in particular relatesto the preparation method of the macadimia nut protein and application of the preparation method. The preparation method comprises the following steps: preparing degreased nut bran, performing enzymetreatment on a material twice, performing alkali dissolution and acid isolation, and the like. By adopting the preparation method, proteins in the degreased nut bran can be efficiently extracted, theproteins and polysaccharides can be effectively separated, and the quality of the obtained macadimia nut protein can be improved. The preparation method is applicable to production practice for preparing healthcare products with the macadimia nut protein or peptide.

Description

technical field [0001] The invention belongs to the field of plant natural product extraction, and relates to a preparation method and application of plant protein, in particular to a preparation method and application of macadamia nut protein. Background technique [0002] Macadamia integrifolia F.Muell (also known as macadamia nut) fruit oil contains a large amount of unsaturated fatty acids, so macadamia nuts are often used to extract natural vegetable oils. However, a large amount of defatted nut meal will be produced after oil extraction. If it is directly discarded, it will cause huge waste and environmental pollution. Defatted nut meal contains a large amount of protein, which contains 17 kinds of amino acids, 10 of which are amino acids that cannot be synthesized by the human body and must be supplied by food. Moreover, the proportion of essential amino acids in its protein composition just meets the needs of the human body, and the utilization rate of this type of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J1/00A23L33/185C12P21/06C07K1/30C07K1/14
CPCA23J1/005A23L33/185C12P21/06C07K1/30C07K1/145A23V2002/00A23V2200/30A23V2250/548
Inventor 徐斌施蕊万举河卢淼马宁李彪
Owner JIANGCHENG ZHONGAO AGRI TECH DEV
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