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Method for preparing bone gelatin

A technology of bone gelatin and bone powder, which is applied to the preparation method of peptides, chemical instruments and methods, animal/human protein, etc., can solve the problems of time-consuming and cumbersome steps, reduce production costs, simplify production processes, and reduce water consumption Effect

Active Publication Date: 2020-01-07
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the existing method for producing bone gelatin by enzymatic hydrolysis still has defects such as time-consuming (about 10-20 hours) and cumbersome steps (for details, see the patent application texts with publication numbers CN201610122663 and CN201110225294), these defects Both will cause adverse effects on the industrialization process of enzymatic production of bone gelatin. Therefore, if the process of enzymatic production of bone gelatin can be further improved so that it can completely replace the process of alkaline production of bone gelatin, it will be of great significance to the production of bone gelatin.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Specific steps are as follows:

[0043] Take the defatted bone grains, and grind the defatted bone grains to a particle size of 0.6 mm with a pulverizer to obtain bone powder;

[0044] (2) Pour the bone meal obtained in (1) into a dilute hydrochloric acid solution with a mass percent concentration of 3% (the mass ratio of bone meal to dilute hydrochloric acid is 1:9), and add 0.2 ‰ of pepsin (at this time, the pH is 2±0.2), enzymatically hydrolyzes collagen at a temperature of 30°C and a stirring speed of 200r / min for 4 hours, and removes hydroxyapatite while completing enzymatic hydrolysis of collagen;

[0045] (3) Centrifuge the mixed solution obtained in (2) at a rotational speed of 5000r / min to remove the supernatant acid solution, wash the remaining precipitate with water to remove impurities, and obtain enzymatic bone powder;

[0046] (4) Mix the enzymatically decomposed bone powder and water at a mass ratio of 1:2 and adjust the pH to 5, then extract the gelatin...

Embodiment 2

[0051](1) Take the defatted bone grains, and use a pulverizer to pulverize the defatted bone grains to a particle size of 0.3 mm to obtain bone meal;

[0052] (2) Pour the bone meal obtained in (1) into a dilute hydrochloric acid solution with a concentration of 3.5% by mass (the mass ratio of bone meal to dilute hydrochloric acid is 1:7), and add 0.2 ‰ of pepsin and acid protease compound enzyme (the pH at this time is 2±0.2), enzymolysis at 28°C and stirring speed of 150r / min for 4h, and complete enzymolysis of collagen while removing hydroxyapatite, wherein, The mass ratio of pepsin and acid protease is 1:1;

[0053] (3) Centrifuge the mixed solution obtained in (2) at a rotational speed of 5000r / min to remove the supernatant acid solution, wash the remaining precipitate with water to remove impurities, and obtain enzymatic bone powder;

[0054] (4) Mix the enzymatic bone meal and water at a mass ratio of 1:2 and adjust the pH to 4.8, then extract the gelatin at 60°C and 2...

Embodiment 3

[0059] (1) Take the defatted bone grains, and use a pulverizer to pulverize the defatted bone grains to a particle size of 0.5 mm to obtain bone meal;

[0060] (2) Pour the bone powder obtained in (1) into a dilute hydrochloric acid solution with a concentration of 3.5% by mass (the mass ratio of bone powder to dilute hydrochloric acid is 1:11), and add 0.2 ‰ of pepsin and acid protease compound enzyme (the pH at this time is 2±0.2), enzymatic hydrolysis at a temperature of 30°C and a stirring speed of 150r / min for 4 hours, and complete enzymatic hydrolysis of collagen while removing hydroxyapatite, wherein, The mass ratio of pepsin and acid protease is 1:1;

[0061] (3) Centrifuge the mixed solution obtained in (2) at a rotational speed of 5000r / min to remove the supernatant acid solution, wash the remaining precipitate with water to remove impurities, and obtain enzymatic bone powder;

[0062] (4) Mix the enzymatic bone powder and water at a mass ratio of 1:2 and adjust the...

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Abstract

The invention discloses a method for preparing bone gelatin and belongs to the technical field of gelatin preparation. By adopting the method disclosed by the invention, three steps (acid pickling, liming and deliming) of a pretreatment process of a traditional alkali method for producing bone gelatin and two steps (acid pickling demineralization and enzymolysis) of a pretreatment process of a conventional enzyme method for producing bone gelatin can be simplified into one step (acid pickling demineralization and enzymolysis are implemented simultaneously), so that the production cycle of thebone gelatin can be greatly shortened, and meanwhile, the production process of the bone gelatin can be simplified; the pretreatment process of the alkali method for producing the bone gelatin takes 20-60 days, the pretreatment process of the conventional enzyme method for producing the bone gelatin takes 15-25 hours, and the pretreatment process of the enzyme method disclosed by the invention forproducing the bone gelatin only takes 3-6 hours. In addition, compared with the conventional alkali method, the enzyme method disclosed by the invention is capable of reducing about 2.6 tons of alkali consumption and 60% (about 300 tons) of water consumption when 1 ton of the bone gelatin is produced.

Description

technical field [0001] The invention relates to a method for preparing bone gelatin, which belongs to the technical field of gelatin preparation. Background technique [0002] Bone gelatin refers to the product obtained by purifying the collagen in animal bone after moderate hydrolysis. It is mainly used in the preparation of pharmaceutical products, such as soft capsules, hard capsules, and tablets. [0003] At present, the production of bone gelatin at home and abroad mainly adopts the alkaline production process, that is, the transformation of bone collagen into bone gelatin is realized through long pretreatment processes such as pickling, liming, and deliming. However, this process takes a long time (it takes about 20 ~60d), large water consumption (about 500t of water is needed to produce 1t of bone gelatin), and, due to the serious energy consumption of sewage treatment, this process will cause serious environmental pollution. Therefore, it is urgent to improve the pro...

Claims

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Application Information

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IPC IPC(8): C07K14/78C12P21/06C07K1/36C07K1/34C07K1/18
CPCC07K14/78C12P21/06
Inventor 赵伟马艳丽
Owner JIANGNAN UNIV