Application of bacillus BCJB01 and BMJBN02 to production of strawberries

A technology of Bacillus megaterium and Bacillus megaterium, applied in application, microorganism-based methods, bacteria and other directions, can solve problems such as damage, hidden dangers of food safety, waste of resources and environment, and achieve simple preservation methods, reduce morbidity, and promote strawberry growth. Effect

Active Publication Date: 2020-01-07
BIOTECH CENT OF SHANDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

During the planting process, in order to increase the yield, fruit growers use a large amount of chemical fertilizers or even abuse them. Although short-term economic benefits are achieved, in the long run, it not only aggravates soil acidification and compaction and soil fertility decline, but also causes waste of resources and The destruction of the environment also poses a great hidden danger to food safety.

Method used

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  • Application of bacillus BCJB01 and BMJBN02 to production of strawberries
  • Application of bacillus BCJB01 and BMJBN02 to production of strawberries
  • Application of bacillus BCJB01 and BMJBN02 to production of strawberries

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1 is applied in the preparation of the bacterial agent of bacillus BCJB01 and BMJBN02 in strawberry production

[0030] The application of Bacillus BCJB01 and BMJBN02 in strawberry production involves the preparation of Bacillus cereus BCJB01 wettable powder and Bacillus megaterium BMJBN02 wettable powder, which is carried out by the following steps:

[0031] (1) Streak and inoculate Bacillus cereus BCJB01 and Bacillus megaterium BMJBN02 stored in an ultra-low temperature refrigerator on solid LB medium respectively, culture overnight at 30°C±1°C in the dark, and pick a single colony into the liquid In LB medium, cultivate to the logarithmic growth phase at 30°C±1°C, the OD value is 0.6-0.8, and the concentration of the seed solution is not less than 1.0×10 8 cfu / mL, respectively to obtain the seed solution to be inserted into the fermentation medium;

[0032] (2) Bacillus cereus BCJB01 fermentation medium formula: corn flour 2%, soybean meal 1%, yeast powde...

Embodiment 2

[0037] Application of embodiment 2 bacterial agents in industrialization strawberry seedling cultivation

[0038] The application of the bacterial agent of bacillus BCJB01 and BMJBN02 in factory strawberry seedling cultivation is carried out by the following steps:

[0039] (1) Add the wettable powder of Bacillus cereus BCJB01 and the wettable powder of Bacillus megaterium BMJBN02 to the seedling cultivation substrate at 10 g / L respectively, and mix well to obtain the cultivation substrate I (containing Bacillus cereus cereus BCJB01 wettable powder) and cultivation medium II (containing Bacillus megaterium BMJBN02 wettable powder), sow and manage Xingdu No. The formula of the seedling raising substrate produced: organic matter content≥45%, humic acid content≥15%, pH value: 5.5-6.8, free water content≤20%) as the No. 2 cultivation group of Xingdu as cultivation substrate as negative control, use The Xingdu No. 2 cultivation group in which compound fertilizer (50kg / mu of superp...

Embodiment 3

[0048] Application of embodiment 3 inoculum in greenhouse strawberry (product of the present invention is compared with common chemical fertilizers in the field)

[0049] The application of the bacterial agent of Bacillus BCJB01 and BMJBN02 in the strawberry in greenhouse is carried out through the following steps:

[0050] (1) The subject of the test is the high-yielding variety Xingdu No. 2 seedlings of Example 2 (1) with consistent growth. The test was carried out in the greenhouse of Sunjia Village, Jinan City, Shandong Province, and the test time was December 2018.

[0051] (2) There are 6 treatments in total in the test: weeds should be thoroughly removed before planting in the greenhouse soil. The Xingdu No. 1 seedlings treated with Bacillus cereus BCJB01 in Example 2 (1) are used as seedlings, and Bacillus cereus is respectively set Bacillus cereus BCJB01 treatment (wettable powder 3.0kg / mu), Bacillus megaterium BMJBN02 treatment (wettable powder 3.0kg / mu), strawberry ...

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Abstract

The invention relates to the technical field of application of microorganisms, in particular to an application of bacillus BCJB01 and BMJBN02 to production of strawberries. The bacillus comprises Bacillus cereus BCJB01 and Bacillus megaterium BMJBN02. The bacterial strain is separated and preserved by a laboratory. The notable characteristic lies in that microbial inoculum is subjected to strawberry factory seedling raising, planting in a strawberry green house, and outdoor planting, and is notable in effect of promoting growth for strawberry factory seedling raising, the root / shoot ratio is notably increased, and is separately increased by 30.93% and 21.90% compared with that of a blank control group. In addition, the strawberries are planted and directly applied by the method, so that yield can be increased by 15-25%. Through combination of the method and fertilizer application reduction to strawberry planting, the yield can be increased by 22-28%, and besides, the method also has the characteristic of controlling powdery mildew of the strawberries, and has great application value for culture of the strawberries.

Description

technical field [0001] The invention relates to the technical field of application of microorganisms, in particular to the application of bacillus BCJB01 and BMJBN02 in strawberry production. Background technique [0002] Strawberry is a perennial herb of Rosaceae, which is rich in nutrients and has a sweet and sour taste, so it is loved by people. With its advantages of easy reproduction, short cultivation period, and high income, strawberry planting is widely carried out in various places in my country, especially in northern regions such as Shandong, Hebei, and Xinjiang. In recent years, my country's strawberry production has taken the lead in the world. During the planting process, in order to increase the yield, fruit growers use a large amount of or even abuse chemical fertilizers. Although short-term economic benefits are achieved, in the long run, it not only aggravates soil acidification and compaction and soil fertility decline, but also causes waste of resources ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05G3/00A01G22/05A01G24/20A01G24/10A01G24/30C12R1/085C12R1/11
CPCC12N1/20C05G3/00A01G22/05A01G24/20A01G24/10A01G24/30
Inventor 谢雪迎张新建周方园王加宁郭书海周红姿范素素赵晓燕张广志
Owner BIOTECH CENT OF SHANDONG ACAD OF SCI
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