Establishment method of improved allogeneic vein transplantation model in rats

Abstract

The invention discloses an establishment method of an improved allogeneic vein transplantation model in rats. The establishment method of the improved allogeneic vein transplantation model in rats comprises the following steps: carrying out field preparation of anesthetics, weighing a rat, and carrying out intraperitoneal injection of the anesthetics at certain amount according to the weight of the rat so as to have the rat anesthetized; putting the anesthetized rat onto a temperature-controlled blanket, and fixing the rat on an operation table; ensuring that the body of the rat is straight and tension-free, shaving off hairs on the chest of the rat by using a shaver, and carrying out local disinfection by using Anerdian (a skin disinfectant); making an anterior incision, of which the length is about 3cm, on the right sternocleidomastoid muscle of the rat so as to have the skin and the subcutaneous tissue cut open; and then, separating out the superior vena cava in the rat, cutting offthe distal end of the superior vena cava, and flushing the venous lumen by using heparin saline of which the concentration is 5000 U / L. The establishment method of the improved allogeneic vein transplantation model in rats allows direct contact of the inner walls of the artery and the vein, so that relatively large anastomosis area is ensured so as to prevent the blood vessels from necrosis; andmoreover, the vascular cuff does not contact with the blood, and the vascular bridge is completely the vein after the transplantation, so that there is no risk of vascular blockage. Therefore, successrate of vein allografts in rats is improved; and mechanism of clinical vascular transplantation is better met.

Description

technical field [0001] The invention relates to the technical field of animal model establishment, in particular to a method for establishing an improved rat allogeneic vein transplantation model. Background technique [0002] Vascular grafting is a common surgical method when there is a defect in the blood vessel that cannot be directly sutured, including embedding the grafted blood vessel in the defect of the blood vessel, first suturing one end of the blood vessel to cut off the excess blood vessel, and then suturing the other end of the blood vessel. The transplanted blood vessel can be autologous Arteries, veins, or allogeneic arteries and veins, or artificial blood vessels, but in microvascular surgery, autologous arteries and veins are still the most commonly used. Rats are not easy to form thromboembolism due to their fast heart rate and fast blood flow. It is often used to establish a pathophysiological model simulating human coronary artery bypass grafting. In the ...

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Problems solved by technology

[0003] The purpose of the present invention is to provide a method for establishing an improved rat allogeneic vein graft model, to solve the problem that the existing vein graft model proposed in the above-mentioned background technology forms a section of vascular bridge between the arterial and venous vessels after transplantation. In line with the research mechanism, it is easy to block, and the position where the arterial blood vessel wall and the blood vessel cover are in contact, and the position where the venous blood vessel wall and the blood vessel cover are in contact is prone to blood vessel necrosis