Establishment method of improved allogeneic vein transplantation model in rats

A technique for vein transplantation and establishment of methods, applied in medical science, veterinary instruments, veterinary surgery, etc., can solve the problems of easy necrosis of blood vessels, easy blockage, and inconsistent with the research mechanism, and achieves short operation time, less trauma, and improved success rate effect

Pending Publication Date: 2020-01-10
LABREAL BIOTECH KUNMING CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a method for establishing an improved rat allogeneic vein graft model, to solve the problem that the existing vein graft model proposed in the above-mentioned background technology forms a section of vascular bridge between the arterial and venous vess

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A method for establishing an improved rat allogeneic vein transplantation model, comprising the following steps:

[0024] S1: The anesthetic is prepared now, the rat is weighed, and a certain amount of anesthetic is injected intraperitoneally according to the weight of the rat to anesthetize the rat;

[0025] S2: Put the rat on a temperature-controlled blanket and fix it on the operating table to ensure that the body of the rat is straight and without tension. The hair on the chest of the rat is scraped off with a hair clipper, and the rat is locally sterilized with anal iodine;

[0026] S3: Take an incision about 3 cm in front of the right sternocleidomastoid muscle of the rat, cut the skin and subcutaneous tissue, separate the superior vena cava of the rat, cut off the distal end of the vein, and rinse the vein with 5000 U / L heparin saline lumen, and immersed in heparin solution for rinsing;

[0027] S4: Prepare a vascular sleeve with an epidural anesthesia catheter,...

Embodiment 2

[0037] A method for establishing an improved rat allogeneic vein transplantation model, comprising the following steps:

[0038] S1: The anesthetic is prepared now, the rat is weighed, and a certain amount of anesthetic is injected intraperitoneally according to the weight of the rat to anesthetize the rat;

[0039] S2: Put the rat on a temperature-controlled blanket and fix it on the operating table to ensure that the body of the rat is straight and without tension. The hair on the chest of the rat is scraped off with a hair clipper, and the rat is locally sterilized with anal iodine;

[0040] S3: Take an incision about 3 cm in front of the right sternocleidomastoid muscle of the rat, cut the skin and subcutaneous tissue, separate the superior vena cava of the rat, cut off the distal end of the vein, and rinse the vein with 5000 U / L heparin saline lumen, and immersed in heparin solution for rinsing;

[0041] S4: Prepare a vascular sleeve with an epidural anesthesia catheter,...

Embodiment 3

[0051] A method for establishing an improved rat allogeneic vein transplantation model, comprising the following steps:

[0052] S1: The anesthetic is prepared now, the rat is weighed, and a certain amount of anesthetic is injected intraperitoneally according to the weight of the rat to anesthetize the rat;

[0053] S2: Put the rat on a temperature-controlled blanket and fix it on the operating table to ensure that the body of the rat is straight and without tension. The hair on the chest of the rat is scraped off with a hair clipper, and the rat is locally sterilized with anal iodine;

[0054] S3: Take an incision about 3 cm in front of the right sternocleidomastoid muscle of the rat, cut the skin and subcutaneous tissue, separate the superior vena cava of the rat, cut off the distal end of the vein, and rinse the vein with 5000 U / L heparin saline lumen, and immersed in heparin solution for rinsing;

[0055] S4: Prepare a vascular sleeve with an epidural anesthesia catheter,...

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Abstract

The invention discloses an establishment method of an improved allogeneic vein transplantation model in rats. The establishment method of the improved allogeneic vein transplantation model in rats comprises the following steps: carrying out field preparation of anesthetics, weighing a rat, and carrying out intraperitoneal injection of the anesthetics at certain amount according to the weight of the rat so as to have the rat anesthetized; putting the anesthetized rat onto a temperature-controlled blanket, and fixing the rat on an operation table; ensuring that the body of the rat is straight and tension-free, shaving off hairs on the chest of the rat by using a shaver, and carrying out local disinfection by using Anerdian (a skin disinfectant); making an anterior incision, of which the length is about 3cm, on the right sternocleidomastoid muscle of the rat so as to have the skin and the subcutaneous tissue cut open; and then, separating out the superior vena cava in the rat, cutting offthe distal end of the superior vena cava, and flushing the venous lumen by using heparin saline of which the concentration is 5000 U/L. The establishment method of the improved allogeneic vein transplantation model in rats allows direct contact of the inner walls of the artery and the vein, so that relatively large anastomosis area is ensured so as to prevent the blood vessels from necrosis; andmoreover, the vascular cuff does not contact with the blood, and the vascular bridge is completely the vein after the transplantation, so that there is no risk of vascular blockage. Therefore, successrate of vein allografts in rats is improved; and mechanism of clinical vascular transplantation is better met.

Description

technical field [0001] The invention relates to the technical field of animal model establishment, in particular to a method for establishing an improved rat allogeneic vein transplantation model. Background technique [0002] Vascular grafting is a common surgical method when there is a defect in the blood vessel that cannot be directly sutured, including embedding the grafted blood vessel in the defect of the blood vessel, first suturing one end of the blood vessel to cut off the excess blood vessel, and then suturing the other end of the blood vessel. The transplanted blood vessel can be autologous Arteries, veins, or allogeneic arteries and veins, or artificial blood vessels, but in microvascular surgery, autologous arteries and veins are still the most commonly used. Rats are not easy to form thromboembolism due to their fast heart rate and fast blood flow. It is often used to establish a pathophysiological model simulating human coronary artery bypass grafting. In the ...

Claims

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Application Information

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IPC IPC(8): A61D1/00
CPCA61D1/00
Inventor 孔令泽唐梅李慧贺永胜
Owner LABREAL BIOTECH KUNMING CO LTD
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