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Application of TRPC1 peptide molecule in preparing drugs for treating inflammation caused by viral infection

A virus infection, molecular technology, applied in the direction of antiviral agents, drug combinations, peptide/protein components, etc.

Active Publication Date: 2020-01-10
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is no application of the peptide molecule C1E3p of the third ectodomain of TRPC1 in the treatment of inflammation caused by viral infection at home and abroad.

Method used

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  • Application of TRPC1 peptide molecule in preparing drugs for treating inflammation caused by viral infection
  • Application of TRPC1 peptide molecule in preparing drugs for treating inflammation caused by viral infection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 C1E3p can significantly inhibit the colocalization of TRPC1 and HSV-1gD

[0023] Experimental method: Fluorescent staining of TRPC1 short peptide (C1E3p, AA608-616 called C1E3p) on HEp-2 cells: plant HEp-2 cells in confocal culture dishes, divide them into control group and experimental group, and treat the cells When the fusion degree reaches about 80%, the control group is directly infected with 0.5MOI HSV-1 at 37°C for 3 hours, and the experimental group is incubated with the drug C1E3p (10μM) and HSV-1 for 30min before infecting the cells at 37°C. 3h; Primary antibody used for cell fluorescence staining: Anti-TRPC1 rabbit polyclonal antibody and Anti-HSV gD mouse polyclonal antibody mixed in 5% BSA solution, the dilution ratio is 1:200; Secondary antibody: AF 488Donkey anti Mouse antibody and AF 546Donkey antiRabbit antibody Mixed in 5% BSA solution, the dilution ratio is 1:200. Fret imaging was performed under a confocal microscope.

[0024] Experimenta...

Embodiment 2

[0026] Example 2 C1E3p can effectively block the interaction of TRPC1-gD and inhibit HSV-1 infection

[0027] Experimental method: Construction of mouse ocular infection virus model: 20 mice were divided into 4 groups of A1, A2, B1, and B2, with 5 mice in each group. Among them, A1 and B1 are the TRPC1 WT control group, and A2 and B2 are the C1E3p treatment group. All mice were anesthetized with 20% urethane solution, the anesthesia dose was 0.1 mL / 10 g, and the right cornea of ​​the mice was scratched with a syringe needle. Mix normal saline with 10 4 Pfu / mouse of HSV-117syn+ was incubated for 30 minutes, and was dropped into the right cornea of ​​mice in group A1. Combine 10 μM C1E3p with 10 4 Pfu / mouse of HSV-1 17syn+ was incubated for 30 minutes and dripped into the right cornea of ​​mice in group A2. Mix normal saline with 10 3 The pfu / mouse of HSV-1 McKrae was incubated for 30 minutes and dripped into the right cornea of ​​mice in group B1. Combine 10 μM C1E3p with...

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Abstract

The invention discloses application of TRPC1 peptide molecule in preparing drugs for treating inflammation caused by viral infection, and belongs to the technical field of biomedicine. Studies show that the third extracellular domain polypeptide of TRPC1 (C1E3p) can significantly inhibit the co-localization of RPC1 and HSV-1 gD, therefore, the degree of HSV-1 entering into cells can be reduced significantly. The HSK of mouse can be reduced obviously after being treated by C1E3p in mouse stromal keratitis (hsk) model constructed by HSV-1 infection. H&E dyeing also show C1E3p can relieve the symptoms of inflammation in sclera, choroid, and retina and detachment of retina, and the survival rate of the mouse is improved significantly. Therefore, polypeptide C1E3p has significant meaning in drug studies for treating inflammation caused by HSV-1 and other similar virus infections.

Description

technical field [0001] The invention belongs to the technical field of biopharmaceuticals, and in particular relates to the application of a TRPC1 peptide molecule in the preparation of medicines for treating inflammation caused by viral infections. Background technique [0002] Herpes simplex virus type 1 (Herpes simplex virus type 1, HSV-1) is an enveloped DNA virus that threatens human health for a long time. HSV-1 is ubiquitous in nature, and the human body is its main host. The virus has a wide range of infection sites, and enters the human body mainly through direct contact with skin and mucous membranes or sexual contact, and can establish a lifelong latent infection in the host's trigeminal ganglion, which is prone to recurrence. In humans, common symptoms of HSV-1 infection are pharyngitis, tonsillitis, and today it remains a huge global medical burden with potentially significant morbidity in humans, however, clinically, there is currently no Effective drugs or m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/08A61P29/00A61P31/22
CPCA61K38/08A61P29/00A61P31/22
Inventor 马鑫毛爱琴何冬旭蔡燕飞
Owner JIANGNAN UNIV
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