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A kind of Bacillus megaterium for preventing and controlling plant nematode diseases and its application

A technology of Bacillus megaterium and plant nematodes, applied in the field of microorganisms, can solve the problems that the ideal effect cannot be exerted, and the biological control effect varies greatly.

Active Publication Date: 2022-07-12
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Most biocontrol fungi have good control effects in indoor and pot experiments, but in field control experiments, they are often limited by various factors such as colonization ability, temperature, humidity, etc.
Bacteria generally have the characteristics of easy cultivation, fast reproduction, and strong colonization ability, and are important nematode biocontrol bacteria resources. However, the biocontrol effects of different bacterial strains vary greatly, and they are strain-specific. Screen efficient, broad-spectrum nematode antagonistic bacterial strains , the development of its cultivation and application technology is an important problem to be solved in the research and application of biological control of nematode diseases

Method used

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  • A kind of Bacillus megaterium for preventing and controlling plant nematode diseases and its application
  • A kind of Bacillus megaterium for preventing and controlling plant nematode diseases and its application
  • A kind of Bacillus megaterium for preventing and controlling plant nematode diseases and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1: Isolation and screening of Bacillus megaterium

[0019] 1. Preparation of Medium

[0020] The formula of common beef extract peptone agar medium is: beef extract 5g; peptone 10g; sodium chloride 5g; agar powder 20g; distilled water, 1L. The pH was adjusted to 7.2, and the cells were sterilized at 121°C for 20 min.

[0021] The formula of enriched beef extract peptone medium is: beef extract 10g; peptone 20g; sodium chloride 5g; distilled water, 1L; high temperature sterilization at 121°C for 20min.

[0022] 2. Isolation and screening of Bacillus megaterium

[0023] Weigh 500 g of soil samples from wheat cyst nematode fields and place them in a pot, rinse with tap water, stir, pour the upper layer of water into the sieve (upper layer 40 mesh sieve, lower layer 60 mesh sieve), and repeat the operation five times. The soil sample retained by the 60 mesh sieve was rinsed into a beaker with a small amount of water. After mixing, pour it into a porous plastic d...

Embodiment 2

[0036] Example 2 Identification of Bacillus megaterium BCCX15

[0037] 1. Morphological identification and detection of physiological and biochemical characteristics

[0038] The isolated strains were inoculated on beef extract peptone plates, incubated at a constant temperature of 28°C for 18-48 hours, the characteristics of the colonies were observed, the bacteria were picked to prepare smears, and Gram staining was performed to observe the morphology and spore formation microscopically. Morphological identification was performed. Sugar (alcohol) fermentation test, Voges-Proskauer test (V-P test, V.P. test), methyl red test (M.R test), indole test, citrate utilization test, starch hydrolysis test, gelatin test were carried out by conventional methods Hydrolysis test, contact enzyme test, tyrosine hydrolysis test (Dong Xiuzhu, Cai Miaoying. System Identification Manual of Common Bacteria, First Edition. Beijing, Science Press, 2001).

[0039] The results showed that the col...

Embodiment 3

[0067] Example 3 The culture conditions of Bacillus megaterium BCCX15 cells

[0068] Set the initial pH values ​​to be 5.5, 6.0, 6.5, 7.0, 7.5, 8.0, 8.5, 9.0, and 9.5, the inoculum amounts to be 1%, 3%, 5%, 7%, and 9%, respectively, and the bottling amounts to be 10%, 10%, and 10%, respectively. 20%, 30%, 40%, 50%, 3 repetitions per treatment, shake culture in liquid medium enriched with beef extract peptone at 28°C and 140rpm for 24-72h and take samples, measure OD600 value, and study different initial pH values , Inoculum amount, bottling amount on the number of bacteria. The results showed that when the initial pH value of the enriched beef extract peptone liquid medium was adjusted to 7.5, the inoculum amount was 3%, and the bottling amount was 10%, it was beneficial to the growth of bacteria, and the number of bacteria was the largest, which was the optimal culture condition.

[0069] To further compare the growth of bacteria under the optimal culture conditions and conv...

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Abstract

The invention discloses a Bacillus megaterium and its application in preventing and treating plant nematode diseases. application. The Bacillus megaterium colonies are milky white, round or approximately round, with transparent serrated edges, uniform texture, milky, moist and flat. Bacillus megaterium BCCX15 is rod-shaped and arranged in a chain; spore-forming, spore-mesogenic, short-column-shaped, and Gram-positive; the Bacillus megaterium can utilize sucrose but not lactose. The Bacillus megaterium provided by the invention is a broad-spectrum nematode antagonistic strain, and the prepared fungicide can effectively prevent and control plant nematode diseases, and also has certain effects of promoting crop growth and increasing yield, and is of great significance in the prevention and control of plant nematode diseases.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and relates to a Bacillus megaterium and its application in preventing and treating plant nematode diseases. Background technique [0002] Plant parasitic nematodes, or plant pathogenic nematodes for short, are widely distributed and have many hosts. They infect plant root tissues and often form root knots or cysts, resulting in plant nematode diseases, which are more harmful than bacteria and viruses, second only to fungal diseases. The global annual crop loss caused by plant-parasitic nematodes is as high as 78 billion US dollars. Nematode disease control has become an important issue in agricultural and forestry production. Plant nematodes mainly include root-knot nematodes and cyst nematodes. Among them, root-knot nematodes have a wide range of hosts. According to incomplete statistics, root-knot nematodes have more than 3,000 species of plants, such as Solanaceae, Cucurbitaceae, Cruci...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/22A01P5/00C12R1/11
CPCC12N1/20A01N63/00C12R2001/11C12N1/205Y02A40/10
Inventor 咸洪泉张启航杨亚楠赵洪海李雅华梁晨
Owner QINGDAO AGRI UNIV
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