Digital PCR reagent kit and detection method for quantitative detection of EB virus nucleic acid

Abstract

The invention belongs to the field of detection of virus nucleic acid, and particularly relates to a digital PCR reagent kit and detection method for quantitative detection of EB virus nucleic acid. The reagent kit contains digital PCR reaction liquid and a quality control product, wherein the digital PCR reaction liquid contains a primer and probe for detecting EB virus nucleic acid, and comprises a forward primer as shown in SEQID NO:1, a reverse primer as shown in SEQID NO:2 and a probe as shown in SEQID NO:3. Fluorophore is marked at two ends of the probe. The reagent kit and the detectionmethod can realize absolute quantitation of nucleic acid molecules, so as to avoid deviation caused by PCR amplification efficiency variance, and the reagent kit and the detection method have high accuracy, sensitivity and repeatability, and easily realize standardization.

Description

technical field [0001] The invention belongs to the field of virus nucleic acid detection, and in particular relates to a digital PCR kit and a detection method for quantitative detection of EB virus nucleic acid. Background technique [0002] Human beings are the only natural host of Epstein-Barr virus (EBV), and the infection rate of EB virus in the world exceeds 90%. Primary infection usually occurs in infancy and is asymptomatic, presenting as an occult infection; however, infectious mononucleosis may result if infection occurs in adolescents or adults. According to reports, more than 95% of the population in my country has been infected with EBV at the age of 3-5. After primary infection, the virus can persist for a long time, showing a latent infection state, which is asymptomatic in most cases. Epstein-Barr virus is mainly transmitted by saliva, but it has also been reported to be transmitted by blood and sexual activity. There are two forms of Epstein-Barr virus-i...

AI Technical Summary

Problems solved by technology

The traditional detection of Epstein-Barr virus-related antibodies is the most commonly used method for diagnosis and monitoring of patients' conditions, but its sensitivity and specificity are poor

The current fluorescent quantitative PCR can only perform relative quantification of the original nucleic acid, and needs to rely on the standard curve or reference gene to determine the amount of nucleic acid, and there are uncertain test results on whether the virus is positive, resulting in multiple tests or test results Inaccurate

Images