The invention discloses a method for improving
linear amplification detection accuracy through multiple detection, a kit and application. The method comprises the following steps: respectively carrying out
linear amplification on a targeted target sequence and a reference sequence of a detection sample and a targeted target sequence and a reference sequence of a
control sample by using multiple primers; linkers are added to the obtained
linear amplification products respectively; performing sequencing; and finally, analyzing a sequencing result by using a standardized
algorithm. According to the method, by reasonably designing primers, carrying out multiple linear amplification detection, standardizing an
algorithm and firstly carrying out linear amplification and then connecting a single-chain connector, accumulation of introduced base errors can be effectively avoided, and the problem of primer preference existing in linear amplification is relieved; target
DNA molecule missing detection is reduced, and the copy number of a target sequence is detected more accurately. The method is widely applicable to CNV detection of any sample possibly containing CNV; particularly, a heterogeneous sample is used for detecting diseases including infectious diseases, cancers and the like.