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Silk fibroin solution preparation and identification method

A technology of protein solution and silk fibroin, which is applied in the field of biomedicine, can solve the problems of poor mechanical properties of regenerated silk fibroin materials, and achieve the effect of shortening the time-consuming identification and rapid elution

Inactive Publication Date: 2020-01-17
长沙凯泽工程设计有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a preparation and identification method of silkworm silk fibroin protein solution, so as to solve the technical problem of poor mechanical properties of the regenerated silk fibroin protein material obtained from the existing regenerated silk fibroin protein solution

Method used

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  • Silk fibroin solution preparation and identification method

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Embodiment 1

[0029] A method for preparing a silkworm fibroin protein solution, which uses silkworm silk as a raw material, and undergoes the steps of degumming, hydrolysis, impurity removal and sterilization in sequence to obtain the silk fibroin protein solution described above, wherein the specific method of the hydrolysis step yes:

[0030] (1) First mix calcium chloride, sodium thiocyanate, distilled water, 1,2,4-butanetriol, polyethylene glycol 300, and diethylenetriaminepentaacetic acid in a mass ratio of 1:0.5:8:2:5 : 0.8 mixed to obtain the solution, for subsequent use;

[0031] (2) Then the degummed silk obtained after the degumming step is successively subjected to freeze-thaw treatment and microwave treatment;

[0032] (3) Finally, add the silk treated in step (2) into a dissolving solution with 6 times the weight, and treat it for 5 hours under the conditions of 70° C. and 0.6 MPa.

[0033] The specific method of degumming treatment is as follows: soak silk in 0.5% sodium ca...

Embodiment 2

[0044] A method for preparing a silkworm fibroin protein solution, which uses silkworm silk as a raw material, and undergoes the steps of degumming, hydrolysis, impurity removal and sterilization in sequence to obtain the silk fibroin protein solution described above, wherein the specific method of the hydrolysis step yes:

[0045] (1) First, calcium chloride, sodium thiocyanate, distilled water, 1,2,4-butanetriol, polyethylene glycol 300, and diethylenetriaminepentaacetic acid are mixed in a mass ratio of 1:0.6:6:3:4 : 1 mixed to obtain a solution, for subsequent use;

[0046] (2) Then the degummed silk obtained after the degumming step is successively subjected to freeze-thaw treatment and microwave treatment;

[0047] (3) Finally, add the silk treated in step (2) into a 4-fold weight solution, and treat it for 8 hours at 80° C. and 0.5 MPa.

[0048] The specific method of degumming treatment is: soak the silk in 1% sodium carbonate aqueous solution with a mass concentrati...

Embodiment 3

[0059] A method for preparing a silkworm fibroin protein solution, which uses silkworm silk as a raw material, and undergoes the steps of degumming, hydrolysis, impurity removal and sterilization in sequence to obtain the silk fibroin protein solution described above, wherein the specific method of the hydrolysis step yes:

[0060] (1) First, calcium chloride, sodium thiocyanate, distilled water, 1,2,4-butanetriol, polyethylene glycol 300, and diethylenetriaminepentaacetic acid are mixed in a mass ratio of 1:0.55:7:2.5:4.5 : 0.9 mixed to obtain a solution, for subsequent use;

[0061] (2) Then the degummed silk obtained after the degumming step is successively subjected to freeze-thaw treatment and microwave treatment;

[0062] (3) Finally, add the silk treated in the step (2) into a dissolving solution with 5 times the weight, and treat it for 6 hours under the conditions of 75° C. and 0.55 MPa.

[0063] The specific method of degumming treatment is: immerse silk in 0.8% so...

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Abstract

The invention provides a silk fibroin solution preparation and identification method. Silkworm silk serves as a raw material and is sequentially degummed, hydrolyzed, washed and sterilized to preparesilk fibroin solution. The step of hydrolysis includes performing freezing and thawing treatment and microwave treatment on the degummed silk obtained after the step of degumming, and then adding thedegummed silk into solving liquid for heating and pressurizing treatment. Strong acid is omitted in the hydrolysis process, damage to a silk structure is avoided, and excellent mechanical performancesare retained. Besides, tangled fibers are sufficiently opened by freezing and thawing treatment and microwave treatment, the silk is sufficiently hydrolyzed by the aid of the subsequent solving liquid, and the complicated step of dialysis is omitted.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a method for preparing and identifying a silkworm silk fibroin protein solution. Background technique [0002] Bombyx mori silk is fibrous, and biomedical materials require diversity and plasticity in the shape of scaffolds. Therefore, the regeneration of natural silk fibers is a necessary step to broaden its application range. At present, silk protein can be processed into ropes, re-growth silk, nanofiber membranes, silk membranes, porous membranes, hydrogels, etc. after degumming and dissolving. Among them, the dissolution of degummed silk is a very critical step, which affects the subsequent silk fibroin. The properties of materials, especially the mechanical properties, are related to the application properties of silk fibroin materials. Silk is mainly composed of sericin and silk fibroin. Sericin is outside and silk fibroin is inside. The content of silk fibroin is 70-8...

Claims

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Application Information

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IPC IPC(8): C07K14/435C07K1/14G01N33/68C08J5/18C08J3/075C08L89/00
CPCC07K14/43586C08J3/075C08J5/18C08J2389/00G01N33/68G01N2333/43578
Inventor 不公告发明人
Owner 长沙凯泽工程设计有限公司
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