Preparation methods and application of euphausia superba iron chelate peptide and iron chelate
A technology of Antarctic krill and Antarctic krill meal, which is applied in the preparation method, application, chemical instrument and method of peptides, etc., can solve the problems of irritation of the gastrointestinal tract, high cost, low bioavailability, etc., and achieves good iron promotion. Absorption, high iron binding capacity, effect of improving absorption rate
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Embodiment 1
[0039] A preparation method of Antarctic krill iron chelating peptide, comprising the steps of:
[0040] S1. Antarctic krill pretreatment: Take 200g of Antarctic krill powder, add 2000mL of n-hexane / absolute ethanol mixture (the volume ratio of n-hexane to absolute ethanol is 3:1), stir at 50°C and 300rpm for 6h , to obtain precipitate A by suction filtration; take the precipitate A and add 2000mL n-hexane / absolute ethanol mixture (the volume ratio of n-hexane to absolute ethanol is 3:1), stir at 50°C and 300rpm for 6h, and suction filter to obtain Precipitation B; naturally air-dry the precipitation B until the water content is 6.9% by weight, and crush it to 80 mesh to obtain Antarctic krill defatted powder, and use the Kjeldahl method to determine the protein content in the Antarctic krill defatted powder ;
[0041] S2. Preparation of Antarctic krill enzymatic hydrolysis solution: Add the Antarctic krill defatted powder described in step S1 into water and mix well to prepa...
Embodiment 2
[0045] Ferrozine used in the following experiments comes from Sangon Bioengineering (Shanghai) Co., Ltd., product number A601542-0001.
[0046] A method for determining the iron binding capacity of Antarctic krill iron chelating peptide, comprising the steps of:
[0047] S1. Accurately weigh 1 mg of the sample to be tested, dissolve it in 1 mL of ultrapure water, prepare a 1 mg / mL sample solution, and use the ultrapure water without sample as a blank control;
[0048] S2. Add 1.35mL ultrapure water to the prepared 1mL sample solution or blank, and then add 50μL 2mM FeCl 2 Solution, shake at room temperature for 10 minutes;
[0049] S3. Add 100 μL of 5 mM Ferrozine aqueous solution, shake at room temperature for 10 minutes, and measure the absorbance at 562 nm. The formula for calculating the iron binding capacity is:
[0050] Iron binding amount (%)=(1-A 样品 / A 空白 )×100; among them, A 样品 is the absorbance value of the sample solution at 562nm, A 空白 is the absorbance value...
Embodiment 3
[0054] The preparation method of Antarctic krill peptide iron chelate comprises steps:
[0055] S1. Take 300 mg of the Antarctic krill iron-chelating peptide with a molecular weight 4 4H 2 O makes Fe 2+ The final concentration was 50mM, and A solution was obtained;
[0056] S2, adding Na to the A solution described in step S1 2 S 2 o 4 To a final concentration of 100mM, to prevent oxidation of ferrous ions, stir at 25°C, pH 6, 150rpm for 30min to obtain B solution;
[0057] S3. Add ethanol to the B solution described in step S2, stir evenly, remove unbound iron ions, centrifuge at 10,000 rpm for 5 minutes to take the precipitate, and vacuum freeze-dry to obtain Antarctic krill peptide iron chelate; wherein, the B solution and The volume ratio of ethanol is 3:17; the vacuum freeze-drying is the first stage precooling section temperature -40 ℃, 2h; the second stage vacuum section temperature -40 ℃, pressure 0Pa, 2h; the third stage lyophilization 25°C, under the condition ...
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