Preparation methods and application of euphausia superba iron chelate peptide and iron chelate

A technology of Antarctic krill and Antarctic krill meal, which is applied in the preparation method, application, chemical instrument and method of peptides, etc., can solve the problems of irritation of the gastrointestinal tract, high cost, low bioavailability, etc., and achieves good iron promotion. Absorption, high iron binding capacity, effect of improving absorption rate

Pending Publication Date: 2020-01-31
DALIAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the first generation of inorganic iron supplements has disadvantages such as low bioavailability, irritating the gastrointestinal tract, and instability. The second generation of organic acid iron supplements has made up for the shortcomings of inorga...

Method used

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  • Preparation methods and application of euphausia superba iron chelate peptide and iron chelate
  • Preparation methods and application of euphausia superba iron chelate peptide and iron chelate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] A preparation method of Antarctic krill iron chelating peptide, comprising the steps of:

[0040] S1. Antarctic krill pretreatment: Take 200g of Antarctic krill powder, add 2000mL of n-hexane / absolute ethanol mixture (the volume ratio of n-hexane to absolute ethanol is 3:1), stir at 50°C and 300rpm for 6h , to obtain precipitate A by suction filtration; take the precipitate A and add 2000mL n-hexane / absolute ethanol mixture (the volume ratio of n-hexane to absolute ethanol is 3:1), stir at 50°C and 300rpm for 6h, and suction filter to obtain Precipitation B; naturally air-dry the precipitation B until the water content is 6.9% by weight, and crush it to 80 mesh to obtain Antarctic krill defatted powder, and use the Kjeldahl method to determine the protein content in the Antarctic krill defatted powder ;

[0041] S2. Preparation of Antarctic krill enzymatic hydrolysis solution: Add the Antarctic krill defatted powder described in step S1 into water and mix well to prepa...

Embodiment 2

[0045] Ferrozine used in the following experiments comes from Sangon Bioengineering (Shanghai) Co., Ltd., product number A601542-0001.

[0046] A method for determining the iron binding capacity of Antarctic krill iron chelating peptide, comprising the steps of:

[0047] S1. Accurately weigh 1 mg of the sample to be tested, dissolve it in 1 mL of ultrapure water, prepare a 1 mg / mL sample solution, and use the ultrapure water without sample as a blank control;

[0048] S2. Add 1.35mL ultrapure water to the prepared 1mL sample solution or blank, and then add 50μL 2mM FeCl 2 Solution, shake at room temperature for 10 minutes;

[0049] S3. Add 100 μL of 5 mM Ferrozine aqueous solution, shake at room temperature for 10 minutes, and measure the absorbance at 562 nm. The formula for calculating the iron binding capacity is:

[0050] Iron binding amount (%)=(1-A 样品 / A 空白 )×100; among them, A 样品 is the absorbance value of the sample solution at 562nm, A 空白 is the absorbance value...

Embodiment 3

[0054] The preparation method of Antarctic krill peptide iron chelate comprises steps:

[0055] S1. Take 300 mg of the Antarctic krill iron-chelating peptide with a molecular weight 4 4H 2 O makes Fe 2+ The final concentration was 50mM, and A solution was obtained;

[0056] S2, adding Na to the A solution described in step S1 2 S 2 o 4 To a final concentration of 100mM, to prevent oxidation of ferrous ions, stir at 25°C, pH 6, 150rpm for 30min to obtain B solution;

[0057] S3. Add ethanol to the B solution described in step S2, stir evenly, remove unbound iron ions, centrifuge at 10,000 rpm for 5 minutes to take the precipitate, and vacuum freeze-dry to obtain Antarctic krill peptide iron chelate; wherein, the B solution and The volume ratio of ethanol is 3:17; the vacuum freeze-drying is the first stage precooling section temperature -40 ℃, 2h; the second stage vacuum section temperature -40 ℃, pressure 0Pa, 2h; the third stage lyophilization 25°C, under the condition ...

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Abstract

The invention discloses a preparation methods euphausia superba iron chelate peptide. Euphausia superba powder is used as a raw material, degreasing, enzymolysis and membrane separation are performed,and a component with molecular weight smaller than 3000 Da is taken and subjected to vacuum freeze-drying to obtain the euphausia superba iron chelate peptide. The euphausia superba iron chelate peptide can bind with Fe<2+> to form euphausia superba iron chelate. The prepared euphausia superba iron chelate peptide has iron chelate rate up to 93%, can improve the bioavailability of iron in organisms, has good iron absorption promoting activity, can promote iron absorption and supplement organisms with essential amino acids for organisms compared with inorganic iron FeSO4, and also provides a new direction for high-value utilization of euphausia superba. The prepared euphausia superba iron chelate peptide can be applied to preparation of iron-rich food or iron supplements.

Description

technical field [0001] The invention relates to the field of preparation of Antarctic krill functional active peptides, in particular to a preparation method and application of an Antarctic krill iron chelating peptide and an iron chelate. Background technique [0002] Antarctic krill has a large yield and rich nutrition, and is currently the most potential marine protein resource. The Antarctic krill enzymatic hydrolyzate is rich in amino acids, rich in essential amino acids, and the ratio of amino acids is reasonable, so it is a good source of human nutritional intake. However, at present, Antarctic krill is mostly used as bait, or used to extract Antarctic krill oil and astaxanthin, and the utilization of Antarctic krill protein is less. [0003] Iron is one of the essential trace elements for the human body. It is widely distributed in the human body and has important biological activities. It participates in various metabolic processes of the human body, such as the tr...

Claims

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Application Information

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IPC IPC(8): A23L33/165A23L33/18A23J1/04A23J3/34C12P21/06C07K1/34A61K38/01A61P3/02
CPCA23L33/165A23L33/18A23J1/04A23J3/341C12P21/06A61K38/012A61P3/02A23V2002/00A23V2250/55A23V2250/1592
Inventor 孙娜王彤彤林松毅崔蓬勃李冬梅姜鹏飞刘柯欣
Owner DALIAN POLYTECHNIC UNIVERSITY
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