Preparation method and application of ganoderma amboinense extract

An extract and antler technology, applied in the field of preparation of antler Ganoderma lucidum extract, can solve the problems of difficulty in collecting the final product, heat loss of the extract, low content of active substances, etc., and achieve the effect that the extraction process is simple and easy to operate

Inactive Publication Date: 2020-02-04
SHENZHEN HUACHUANG BIO PHARM TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The Ganoderma lucidum extract obtained by the solvent extraction method currently used does not have a high content of active substances, and the traditional heating extraction and concentration methods easily lead to its loss of activity
Moreover, there are very few fat-soluble components in the fruiting bodies of Ga

Method used

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  • Preparation method and application of ganoderma amboinense extract

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The preparation of the extract of embodiment 1 staghorn ganoderma lucidum

[0025] Take the dried staghorn ganoderma fruiting bodies, chop them, pass through a 80-mesh sieve, weigh 10g of staghorn ganoderma fruiting body powder, and disperse the staghorn ganoderma fruiting bodies in 100mL 70-90°C according to the liquid-to-material ratio of 10:1 (mL / g) In distilled water, mix evenly, use an ultrasonic crusher: JY92-IIN type, 700W ultrasonic crushing for 1 hour, then filter, extract the filter cake with water for later use, add 5 times the volume of ethanol to the filtrate of the water extraction, stir, and let it settle at 4°C , filter to obtain the alcohol precipitation filter cake as the first extract;

[0026] Concentrate the alcohol precipitation filtrate and combine it with the water-extracted filter cake, add distilled water according to the liquid-to-solid ratio of 15:1 (mL / g), adjust the pH value to 5.6 with citric acid buffer, and add fiber at 20% of the weight...

Embodiment 2

[0027] The preparation of the extract of embodiment 2 staghorn ganoderma

[0028] Take the dried staghorn ganoderma fruiting bodies, chop them, pass through a 50-mesh sieve, weigh 10g of staghorn ganoderma fruiting body powder, and disperse the staghorn ganoderma fruiting bodies in 150mL of 70-90°C distilled water according to the liquid-to-material ratio of 15:1 (mL / g) Medium, mix evenly, use an ultrasonic crusher: JY92-IIN type, 500W ultrasonic crushing for 1.5 hours, then filter, extract the filter cake with water for later use, add 8 times the volume of ethanol to the filtrate from the water extraction, stir, and let it settle at 4°C. Filtration to obtain the alcohol precipitation filter cake is the first extract;

[0029] Concentrate the alcohol precipitation filtrate and combine it with the water extraction filter cake, add distilled water according to the liquid-to-solid ratio of 25:1 (mL / g), adjust the pH value to 5.6 with citric acid buffer solution, and add papaya in...

Embodiment 3

[0030] Embodiment 3 extraction rate experiment

[0031] 3.1 Extraction rate experiment of β-glucan

[0032] The yield of β-glucan was determined by the Congo red method.

[0033] β-glucan yield (mg / g) = β-glucan mass (mg) / raw material mass (g)

[0034] 3.2 Extraction rate experiment of Ganoderma lucidum triterpenes

[0035] The methanol solutions of ganoderma acid A and ganoderma acid B were used as reference substances, and were detected by high performance liquid chromatography. The extraction rate of staghorn ganoderma lucidum triterpenes is calculated based on the sum of the content of ganoderma acid A and ganoderma acid B, and the calculation formula is:

[0036] Extraction rate=(content of ganoderma acid A+content of ganoderma acid B)×volume of extraction solution / mass of raw material.

[0037] 3.3 The experimental results of extraction rate are as shown in Table 1. On the one hand, two groups of extracts can be obtained by adopting the method of the present invent...

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Abstract

The present invention belongs to the technical field of traditional Chinese medicinal material extraction and particularly relates to a preparation method and an application of a ganoderma amboinenseextract. The preparation method comprises the following steps of processing raw materials, obtaining a first extract and obtaining a second extract. The present invention also provides a nutritional product. The nutritional product comprises a first extract and/or a second extract. In a provided technical scheme, two different extraction methods and extraction solvents are used to realize relatively higher extraction rates of ganoderma triterpene and beta glucan in ganoderma amboinense and at the same time roughly separate the two components, which is convenient to adjust addition amount of the two components according to requirements in a later period, besides, a high-temperature and high-pressure extraction method is avoided in an extraction process, the used solvents are also safe and environment-friendly, the extraction process is simple and easy to operate, and the feasible method is provided for effective and efficient utilization of the ganoderma amboinense.

Description

technical field [0001] The invention belongs to the technical field of extraction of traditional Chinese medicinal materials, and in particular relates to a preparation method and application of antler ganoderma extract. Background technique [0002] Antler Ganoderma is a kind of Ganoderma lucidum. It is a kind of fungus with long stipe, club-shaped top, no umbrella-shaped cap or only small nearly spatula-shaped fungus formed by abnormal growth due to changes in external conditions during the cultivation process. A new strain of the cover, multi-branched and shaped like antlers. [0003] The main active ingredients in staghorn ganoderma include ganoderma triterpenes and β-glucan, which can directly inhibit or kill tumors and cancer cells through various effective ways. [0004] β-glucan is a water-soluble substance, while Ganoderma lucidum triterpenoids are fat-soluble components. The Ganoderma lucidum extract obtained by the solvent extraction method currently adopted doe...

Claims

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Application Information

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IPC IPC(8): A61K36/074A61K36/815A61K36/39A61K36/068A61K35/644C08B37/02
CPCA61K35/644A61K36/068A61K36/074A61K36/39A61K36/815A61K2236/19A61K2236/331A61K2236/333A61K2236/51A61K2236/53C08B37/0003C08B37/0024A61K2300/00
Inventor 刘东
Owner SHENZHEN HUACHUANG BIO PHARM TECH CO LTD
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