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Preparation method and application of glucose-oxidase-modified mesoporous manganese dioxide composition

A technology of glucose oxidase and manganese dioxide, which is applied in the field of medicine, can solve problems such as no public reports, and achieve the effects of stable and reliable preparation method, low cost, and huge economic and social benefits

Inactive Publication Date: 2020-02-04
XINXIANG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, inventing a glucose oxidase-modified mesoporous manganese dioxide pharmaceutical composition has great significance and value in tumor diagnosis and treatment, but there has been no public report so far

Method used

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  • Preparation method and application of glucose-oxidase-modified mesoporous manganese dioxide composition

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Effect test

Embodiment 1

[0020] A method for preparing a glucose oxidase-modified mesoporous manganese dioxide pharmaceutical composition of the present invention comprises the following steps:

[0021] (1), preparation of mesoporous manganese dioxide nanoparticles: ultrasonically dissolve 7.8g of sodium dodecylbenzenesulfonate in 100mL of ultrapure water to form a solution of dodecylbenzenesulfonic acid; dissolve 1.4g of sodium dodecylbenzenesulfonate Sodium hydrogen was dissolved in 15mL ultrapure water to form a colorless and transparent solution; 1.5g of MnCl 2 4H 2 O was dissolved in 9mL of ultrapure water to form a pale pink transparent solution; the dodecylbenzenesulfonic acid solution was placed in an oil bath at 50°C and stirred for 7 minutes, then added with sodium bicarbonate solution and continued to stir for 35 minutes, and then added dropwise to the dodecylbenzenesulfonic acid solution Good MnCl 2 4H 2 The O solution was stirred for 3.5 hours, centrifuged at 13,000 rpm for 10 minutes ...

Embodiment 2

[0026] A method for preparing a glucose oxidase-modified metal-organic framework pharmaceutical composition of the present invention is realized by the following steps:

[0027] (1) Preparation of mesoporous manganese dioxide nanoparticles: Weigh 7.75g of sodium dodecylbenzenesulfonate and dissolve it in 100mL of ultrapure water; weigh 1.4g of sodium bicarbonate and dissolve it in 15mL of ultrapure water into a colorless transparent solution; weigh 1.5g of MnCl 2 4H 2 O was dissolved in 9mL of ultrapure water to form a pale pink transparent solution; the dodecylbenzenesulfonic acid solution was placed in an oil bath at 50°C and stirred for 7.5min, then sodium bicarbonate solution was added to continue stirring for 35min, and then added dropwise Prepared MnCl 2 4H 2 The O solution was stirred for 3.5 hours, centrifuged at 12,500 rpm for 10 minutes to collect the precipitate, washed with ultrapure water for 4 times, and then washed with absolute ethanol for 4 times, dried at ...

Embodiment 3

[0032] A method for preparing a glucose oxidase-modified metal-organic framework pharmaceutical composition of the present invention is realized by the following steps:

[0033] (1) Preparation of mesoporous manganese dioxide nanoparticles: Weigh 7.5g of sodium dodecylbenzenesulfonate and dissolve it in 90mL of ultrapure water; weigh 1.3g of sodium bicarbonate and dissolve it in 12mL of ultrapure water into a colorless transparent solution; weigh 1g of MnCl 2 4H 2 O was dissolved in 6mL of ultrapure water to form a pale pink transparent solution; the dodecylbenzenesulfonic acid solution was placed in an oil bath at 50°C and stirred for 5 minutes, then sodium bicarbonate solution was added to continue stirring for 30 minutes, and then the dodecylbenzenesulfonic acid solution was added dropwise. Good MnCl 2 4H 2 The O solution was stirred for 3 hours, centrifuged at 10,000 rpm for 10 minutes to take the precipitate, washed with ultrapure water for 3 times, then washed with ab...

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Abstract

The invention relates to a preparation method and application of a glucose-oxidase-modified mesoporous manganese dioxide composition, and effectively solves the problem of providing new drugs for treating tumor diseases by preparing a glucose-oxidase-modified mesoporous manganese dioxide composition so as to realize synergistic inhibition of tumor cell growth by sonodynamic therapy and starvationtherapy. The preparation method of the glucose-oxidase-modified mesoporous manganese dioxide composition comprises the following steps: modifying surfaces of mesoporous manganese dioxide particles with glucose oxidase through amide bond reaction; physically loading a small-molecular sound sensitizer in pore canals of the mesoporous manganese dioxide, thereby obtaining an internally loaded sensitizer; and then, performing covalent modification on the outer side of the internally loaded sensitizer with glucose-oxidase-modified pharmaceutical composition of mesoporous manganese dioxide nanoparticles. Particle sizes of the glucose-oxidase-modified pharmaceutical composition of the mesoporous manganese dioxide nanoparticles are 100-300 nm; and mass ratio of the glucose oxidase to the manganesedioxide is 1 to 5. The preparation method of the glucose-oxidase-modified mesoporous manganese dioxide composition disclosed by the invention is stable, reliable, and low in cost; and moreover, the glucose-oxidase-modified mesoporous manganese dioxide composition can be used as a magnetic resonance imaging contrast agent for performing real-time monitoring on tumors. Therefore, the glucose-oxidase-modified mesoporous manganese dioxide composition is an innovation in tumor treatment drugs.

Description

technical field [0001] The invention belongs to the field of medicine, and relates to a preparation method and application of a glucose oxidase-modified mesoporous manganese dioxide pharmaceutical composition. Background technique [0002] In recent years, sonodynamic therapy (SDT) has become an emerging means of treating tumors, and sonodynamic therapy is a preferred treatment method developed on the basis of photodynamic therapy (PDT). Compared with PDT, SDT has the characteristics of deep tissue penetration, non-invasiveness, and low toxicity to normal tissues. SDT refers to the administration of a certain dose of sonosensitizer to the patient. Under the excitation of a certain frequency and intensity of ultrasound, the sonosensitizer transitions from the ground state to the excited state, and then releases energy and transfers it to oxygen molecules to generate active oxygen when the excited state returns to the ground state ( ROS), leading to irreversible damage to tum...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K38/44A61K9/51A61K47/02A61K49/08A61P35/00
CPCA61K9/5115A61K38/443A61K41/0033A61K49/08A61P35/00C12Y101/03004A61K2300/00
Inventor 尹延彦马伟伟杨鹏飞王泽颖牛秉轩李娜宋宇梁金英
Owner XINXIANG MEDICAL UNIV
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