Aconitum carmichaeli Debx. homogeneous polysaccharide as well as preparation method and application thereof
A polysaccharide and uniform technology, applied in the field of medicine, can solve the problems of high active ingredients, loss of active ingredients, unclear, etc., and achieve the effect of high yield and strong reducing power measurement
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Embodiment 1
[0034] The preparation method of Paotianxiong uniform polysaccharide comprises the following steps:
[0035]Step 1: Heat the dried Paotianxiong decoction pieces with 5 times the amount of ethanol, heat, condense and reflux twice to degrease, and obtain the dregs after degreasing; Step 2: Extract the dregs twice with 5 times the amount of water at a temperature of 80°C , 1.5 hours each time, after concentrating the water extract under reduced pressure, add absolute ethanol until the alcohol content reaches 80%, let it stand for 18 hours, discard the supernatant, collect the precipitate, and obtain Paotianxiong crude polysaccharide; step 3: Reconstitute the crude polysaccharide of Paotianxiong, add pepsin to its content of 0.1%, put it in a constant temperature water bath at 32°C for 10 hours, remove the precipitate, repeat twice, collect the upper layer solution, concentrate, add Sevage reagent, shake vigorously for 20 minutes, and let stand 8h, collect the polysaccharide solut...
Embodiment 2
[0041] The preparation method of Paotianxiong uniform polysaccharide comprises the following steps:
[0042] Step 1: Heat the dried Paotianxiong decoction pieces with 6 times the amount of ethanol, heat, condense and reflux twice to degrease, and obtain the dregs after degreasing; Step 2: Extract the dregs twice with 7 times the amount of water at a temperature of 90°C , 1.5 hours each time, after concentrating the water extract under reduced pressure, add absolute ethanol until the alcohol content reaches 82%, let it stand for 20 hours, discard the supernatant, collect the precipitate, and obtain the crude polysaccharide of Paotianxiong; step 3: Reconstitute the crude polysaccharide of Paotianxiong, add pepsin until its content is 0.2%, put it in a constant temperature water bath at 35°C for 11 hours, remove the precipitate, repeat twice, collect the upper layer solution, concentrate, add Sevage reagent, shake vigorously for 25 minutes, and let stand 10h, collect the polysacc...
Embodiment 3
[0048] The preparation method of Paotianxiong uniform polysaccharide comprises the following steps:
[0049] Step 1: Heat the dried Paotianxiong decoction pieces with 8 times the amount of ethanol to condense and reflux for 3 times to degrease, and obtain the dregs after degreasing; Step 2: Extract the dregs with 8 times the amount of water for 3 times, the temperature is 100°C , 2 hours each time, after concentrating the water extract under reduced pressure, adding absolute ethanol until the alcohol content reaches 85%, standing for 24 hours, discarding the supernatant, collecting the precipitate, and obtaining the crude polysaccharide of Paotianxiong; step 3: After the crude polysaccharide of Paotianxiong was reconstituted, add pepsin until its content was 0.4%, put it in a constant temperature water bath at 37°C for 12 hours, remove the precipitate, repeat 3 times, collect the upper layer solution, concentrate, add Sevage reagent, shake vigorously for 30 minutes, and let it ...
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