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New trichoderma harzianum bacterial strain

A new strain and strain technology, applied in the field of microorganisms, can solve problems such as soil degradation, environmental pollution, and drug resistance of pathogens

Pending Publication Date: 2020-02-04
ZHEJIANG BIOASIA PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, long-term and extensive use of chemical agents can easily lead to soil degradation, environmental pollution, and pathogenic bacteria to develop drug resistance.

Method used

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  • New trichoderma harzianum bacterial strain

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The isolation of embodiment 1 Trichoderma harzianum strain

[0023] The method of detection and isolation of fungi in soil was used to separate the soil samples of various samples. For the separation method, refer to the method of "P132 (7) Detection and isolation of fungi in soil" in "Methods of Plant Disease Research" edited by Fang Zhongda. The separation medium is: Martin's (Martin) medium: glucose 10g, peptone 5g, KH 2 PO 4 1g, MgSO 4 7H 2 O 0.5g, agar 20g, streptomycin 30.0mg, 1 / 30000 Bengal red aqueous solution 100ml, water 900ml. The various components are dissolved in water, heated, and Bengal red is added when it begins to boil, then aliquoted and sterilized, and streptomycin is added before pouring into plates. For the isolation of fungi from soil.

Embodiment 2

[0024] Embodiment 2 Trichoderma harzianum is to the inhibition rate measurement of Fusarium graminearum

[0025] For more than 200 strains of phytopathogenic fungi isolated from the soil, their inhibitory rate to Fusarium graminearum was measured, and the bacteriostatic ability of Trichoderma harzianum to Fusarium graminearum was measured by the colony confrontation method. The specific methods are as follows:

[0026] ① Prepare a PDA plate, culture the strains to be tested and Fusarium graminearum respectively, and after the plate is basically full, then use a sterilized 4mm puncher to punch holes in the parts where the growth of the two media is consistent ( equidistant from the edge of the dish).

[0027] ② On the empty PDA plate, use sterilized tweezers to pick up the perforated culture medium blocks, invert and inoculate the two fungi, the distances are basically the same, and then measure the size of the inhibition zone every 12 hours (cross method). The antibacterial r...

Embodiment 3

[0036] The cultivation of embodiment 3 Trichoderma harzianum spore powder

[0037] The strain used was BACC0957, and the spores on the slope were inserted into shake flasks for culture. The culture medium was 200g of potato juice, 2% glucose, water to 1L, temperature 25°C, rotation speed 180rpm, and culture for 7 days. When the culture solution in the shake flask is viscous and green to dark green, the culture can be stopped.

[0038]Then connect the seed liquid to the solid medium and ferment for 7 days. Fermentation conditions: temperature 25°C, humidity 95%; solid fermentation medium: bran 4.6%, rice husk 13.9%, corn flour 13.9%, sawdust 4.6%, water 63.0%. The inoculum volume is 20%. After the inoculation, carry out shading and dark culture at 24°C for 2-3 days. When the mycelium grows full, turn to light and culture at 25°C for 5-7 days. When the spore filaments are full and the whole plate is dark green, you can harvest , the solid culture was dried in a drying oven at ...

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Abstract

The invention relates to a new trichoderma harzianum (Trichoderma harzianum) bacterial strain BACC0957. The bacterial strain is preserved in the CGMCC of China Committee for Culture Collection of Microorganisms as a patent strain, wherein the preservation No. is CGMCC NO:15981. The bacteria restraining rate of the bacterial strain on a main pathogeny namely Fusarium graminearum ( Fusarium graminearum ) is as high as 62.97%.

Description

technical field [0001] The invention relates to a new Trichoderma harzianum strain BACC0957, which belongs to the field of microorganisms. Background technique [0002] Trichoderma (Trichoderma spp.) belongs to the Hyphospora class of the half-known fungi and the half-knowledge fungi. It exists in abundance in ecological environments such as plant rhizomes, leaf circles, seeds and bulbs, and has antagonistic effects on a variety of important plant pathogenic fungi, and is considered to be a promising biological control factor. The most widely used Trichoderma is Trichoderma harzianum, which is one of the parasites and antagonists of various plant pathogens. In 1981, in Western Europe and other countries, there were commercial Trichoderma preparations used to prevent and control silver leaf disease of Prunus fruit trees. At the same time, products made from its conidia were used to prevent and control soil-borne diseases such as bacterial blight, blight, and blight. It is c...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N3/00A01P3/00C12R1/885
CPCC12N1/14C12N3/00C12R2001/885C12N1/145Y02A40/10
Inventor 樊美珍彭国杰顾玲玲谭悠久董建飞王莹孙长胜
Owner ZHEJIANG BIOASIA PHARMA CO LTD
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