Taq DNA polymerase mutant and application thereof

Abstract

The invention discloses a Taq DNA polymerase mutant and application thereof. The Taq DNA polymerase mutant has amino acid substitutions at one or more of the following amino acid positions in the sequence shown as SEQ ID NO. 1; and the various amino acid substitutions are represented in triplets as 'letters-numbers-letters', wherein the numbers reflect position of an amino acid mutation, the letters before the numbers are corresponding to an amino acid involved in the mutation, and the letters after the numbers reflect an amino acids used to replace the amino acid corresponding to the lettersbefore the numbers. The Taq DNA polymerase mutant disclosed by the invention is capable of changing configuration of an enzyme, thereby improving tolerance of the enzyme; and thus, the mutant can be very well applied in blood sample amplification.

Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to a Taq DNA polymerase mutant and application thereof. Background technique

[0002] PCR technology has been widely used in many fields such as molecular diagnosis of genetic diseases, animal and plant import and export quarantine, clinical inspection, food safety monitoring, paternity testing and soil microbial detection. The detected gene templates mainly come from tissues, saliva, cells, sputum, blood, feces, and soil. Because there are hemoglobin, heme, lactoferrin, IgG and humic acid in samples such as blood and soil, these substances have a strong inhibitory effect on Taq DNA polymerase. The traditional method is to extract the nucleic acid from these samples and then perform PCR amplification. Due to the increasing detection throughput in this field, the traditional method has many steps, resulting in low work efficiency, time-consuming reagents, high time and financial costs,...

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