Cryopreservation method of autologous skull

Abstract

The invention discloses a cryopreservation method of an autologous skull. The method is used for solving the problem that autologous skulls are polluted easily by a preservation medium in the prior art and can be used to improve bone growth activity. Preservation is carried out by sequentially adopting the following steps: performing pretreatment, performing washing, performing pre-freezing, preparing a gel, performing radiation sterilization, performing freezing storage and performing post-treatment. A preparation process of the gel is combined with preservation of an autologous skull, then the gel with growth activity is embedded in gaps on the surface of the skull, and growth of bones is promoted after replantation. According to the invention, a preparation process of polyvinyl alcohol(PVA) hydrogel carrying nano-hydroxyapatite (n-HA) is combined with preservation of the autologous bone, the PVA hydrogel in a liquid state is in full contact with bone tissues, and the PVA hydrogel forms a gel when the skull is taken out, so that adhesive force of the gel on bones is improved, exogenous pollution is avoided, and bone growth after replantation can be promoted.

Description

technical field [0001] The invention belongs to the technical field of human tissue storage, and in particular relates to a method for cryopreservation of an autologous skull. Background technique [0002] Decompressive craniectomy for hematoma removal or simple decompressive craniectomy is a common neurosurgery operation in primary hospitals. It is of great importance for patients with cerebral hemorrhage and severe craniocerebral injury to pass the peak period of cerebral edema, reduce complications, and reduce mortality. Compared with traditional methods such as titanium mesh and allograft bone repair, autologous skull replantation has more obvious advantages. Not only does the shape need not be readjusted, but also there is no rejection, the wound heals well, and the cost of use is lower, which can ensure the integrity of the skull. sex. However, there is no unified standard for the method of autologous skull preservation. [0003] The existing technology provides a me...

AI Technical Summary

Problems solved by technology

However, there is currently a lack of uniform standards for the preservation of autologous skulls

[0003] The existing technology provides a method for bone tissue preservation: first, the donor is scrubbed with 1‰ geramine solution, and then, in a sterile environment, the muscle, periosteum and other appendages on the bone surface are removed, and rinsed repeatedly with normal saline Next, put the rinsed donor into the plasma bag, seal it with heat, cool down at a rate gradient of 4°C / min, and store it at -80°C. When rewarming, put the bone tissue in 45 ℃ in Ringer’s solution, and then soaked in gentamicin before use; however, because the existing technology is to freeze the donor in plasma, plasma is a good medium, and it is easy to use in aliquots. It is polluted by the outside world and grows slowly in long-term frozen storage, which will affect the use of donors. If the plasma is sterilized, the normal cells in it will also be inactivated, and the role of plasma in maintaining the biological activity of bone tissue will disappear. , even if the donor is put into the plasma, because the density of the donor is higher, it will sink to the bottom and will not be fully contacted. Using plasma will not achieve the expected effect. In addition, this method can only be used for autologous skull preservation. Allogeneic reimplantation can lead to severe rejection

[0004] In addition, after skull replantation, apart from infection, there is also the speed of skull repair. Although the autologous skull will not cause rejection, it will be sterilized before and after preservation, which will affect the activity of the skull. growth factor and promote bone growth; the Chinese journal "Journal of Synthetic Crystals" published an article entitled "Research Progress in the Synthesis and Application of Hydroxyapatite", which introduced the application of hydroxyapatite in bone tissue engineering, and also It has been proved that hydroxyapatite has good osteoinductivity, but its mechanical properties are poor. The current application direction is to compound with other materials to improve the performance of bone tissue, and there is no disclosure of the method of using hydroxyapatite for low-temperature preservation

[0005] Gel materials are widely used in medical treatment due to their unique properties, as wound dressings or slow-release carriers, and are also used in the field of orthopedics. 》The Chinese literature introduced a method of making cartilage using polyvinyl alcohol hydroxyapatite material, but there is no case of using gel material for tissue cryopreservation

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