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Preparation method of cytokine preparation for promoting wound regeneration and repair

A technology of cytokines and wounds, applied in the field of biomedicine, can solve the problems of easy differentiation and low success rate of transplantation, and achieve the effect of reducing susceptibility to contamination and simple preparation methods

Inactive Publication Date: 2020-02-11
安徽科门生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the deficiencies of the prior art, the purpose of the present invention is to provide a preparation method of a cytokine preparation that promotes wound regeneration and repair, which solves the problem in the prior art that differentiation is prone to occur during the preparation process, resulting in a low success rate of transplantation

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  • Preparation method of cytokine preparation for promoting wound regeneration and repair

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Effect test

Embodiment 1

[0028] Such as figure 1 As shown, the embodiment of the present invention provides a preparation method of a cytokine preparation for promoting wound regeneration and repair, comprising the following steps:

[0029] S1. Preparation of tendon stem cell culture medium: 10% by weight of serum, 100 U / mL of penicillin, and 100 U / mL of streptomycin were added to DMEM (containing various amino acids required for nutrition and glucose) medium.

[0030] S2. Preparation of tendon stem cells: Under aseptic conditions, the Achilles tendon tissue pieces were washed with 0.1mol / L phosphate buffer solution, then washed with normal saline, and then cut into 1-2mm blocks, and then placed in digestive fluid Completely submerge for 1 hour, then add tendon stem cell culture medium to obtain mixed solution A, wherein: the digestive solution in S2 is mixed with collagenase and 0.1% trypsin solution in parts by weight at a ratio of 1:1.

[0031] S3. Obtaining primary tendon stem cells: Centrifuge t...

Embodiment 2

[0035] The embodiment of the present invention provides a preparation method of a cytokine preparation for promoting wound regeneration and repair, comprising the following steps:

[0036] S1. Preparation of tendon stem cell culture medium: 10% by weight of serum, 100 U / mL of penicillin, and 100 U / mL of streptomycin were added to DMEM (containing various amino acids required for nutrition and glucose) medium.

[0037] S2. Preparation of tendon stem cells: Under aseptic conditions, the Achilles tendon tissue pieces were washed with 0.1mol / L phosphate buffer solution, then washed with normal saline, and then cut into 1-2mm blocks, and then placed in digestive fluid Completely submerge for 2 hours, and then add tendon stem cell culture medium to obtain mixed solution A, wherein: the digestion solution in S2 is mixed with collagenase and 0.1% trypsin solution in parts by weight at a ratio of 1:1.

[0038] S3. Obtaining primary tendon stem cells: Centrifuge the mixture A obtained i...

Embodiment 3

[0042] The embodiment of the present invention provides a preparation method of a cytokine preparation for promoting wound regeneration and repair, comprising the following steps:

[0043] S1. Preparation of tendon stem cell culture medium: 10% by weight of serum, 100 U / mL of penicillin, and 100 U / mL of streptomycin were added to DMEM (containing various amino acids required for nutrition and glucose) medium.

[0044]S2. Preparation of tendon stem cells: Under aseptic conditions, the Achilles tendon tissue pieces were washed with 0.1mol / L phosphate buffer solution, then washed with normal saline, and then cut into 1-2mm blocks, and then placed in digestive fluid Completely submerge for 1 hour, then add tendon stem cell culture medium to obtain mixed solution A, wherein: the digestive solution in S2 is mixed with collagenase and 0.1% trypsin solution in parts by weight at a ratio of 1:1.

[0045] S3. Obtaining primary tendon stem cells: Centrifuge the mixture A obtained in S2 a...

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Abstract

The invention discloses a preparation method of a cytokine preparation for promoting wound regeneration and repair. The preparation method includes the following steps of S1, preparation of a tendon stem cell culture solution; S2, preparation of tendon stem cells; S3, acquisition of primary tendon stem cells; S4, tendon stem cell culturing; and S5, acquisition of the tendon stem cells. The tendonstem cells are acquired by a direct shear method adopted by preparation of the tendon stem cells, then are placed in a digestive juice to be digested to obtain single cells to avoid excessive accumulation of the single cells, the obtained single cells are cultured separately to reduce multicellular aggregation resulting in inadequate absorption of other local components, inadequate nutrient absorption in cell culture can be prone to leading a differentiation phenomenon, the cell proliferation rate is affected, acquired platelet-rich plasma after centrifugation contains a large amount of growthfactors, and the preparation method can be applied directly.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a preparation method of a cytokine preparation for promoting wound regeneration and repair. Background technique [0002] With the development of society and the improvement of technology, beauty has been constantly changing and improving from the content to the form. According to the different connotations of beauty, modern beauty can be divided into two parts: life beauty and medical beauty. Generally, a series of surgical treatments are performed on the facial skin by means of medical treatment in order to maintain the facial skin for a long time, but this method has certain surgical risks, and the drugs used will remain in the human skin for a long time, which will cause damage to the human body. secondary injury. [0003] With the growth of age, the epidermis of the general human body will continue to fall off and be replenished by the proliferation of basa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/077
CPCC12N5/066C12N2509/00C12N2509/10
Inventor 张鹏成
Owner 安徽科门生物科技有限公司