Application of schizochytrium sp. powder to increment of DHA content in milk of ruminants
A technology of ruminants and Schizochytrium powder, which is applied in application, animal feed, animal feed, etc., can solve the problems of the taste of Schizochytrium powder preparation, the complexity of the fermentation medium composition, and the long feeding cycle, etc., to achieve Improve production performance, improve production efficiency, and easy absorption
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Embodiment 1
[0042] The preparation of embodiment 1 Schizochytrium powder
[0043] The steps for preparing Schizochytrium sp. D1.0 with the preservation number of CCTCC NO.M2016617 in China Typical Culture Collection Center are as follows, as follows: figure 1 As shown, the following steps can be repeated on the fermenter complete set of equipment:
[0044] (1) Preparation of culture medium
[0045] The seed medium is 20-50g / L glucose, 2-4g / L yeast extract, 10-20g / L sodium glutamate, 1.5-2.0g / L sodium chloride, 1.0-2.0g / L magnesium sulfate, and The amount is water, pH5.0-7.0. The prepared medium was sterilized by steam at 121°C, and after 30 minutes, it was lowered to the inoculation temperature for use;
[0046] The fermentation medium is glycerol 50-70g / L, glucose 50-70g / L, yeast extract 4-6g / L, sodium glutamate 10-20g / L, sodium chloride 2.4-4.0g / L, magnesium sulfate 1.0 -2.0g / L, ammonium sulfate 1.0-2.0g / L, the balance is water, pH5.0-7.0. The prepared medium was sterilized by stea...
Embodiment 2
[0069] Embodiment 2 Schizochytrium algae powder feeding dairy cows
[0070] (1) Selection and grouping of experimental animals
[0071] According to the principle of roughly the same or similar age, parity, lactation time and physiological state, 64 healthy Holstein lactating cows in the middle stage of lactation were selected and randomly divided into 4 groups, 16 cows in each group.
[0072] (2) Test diet
[0073] Basic ration—concentrate feed and roughage, keeping the original feeding conditions of the cattle farm: 10kg of concentrate feed, 6.5kg of brewer’s grains as roughage, 22kg of silage, 2.2kg of beet meal, and 2.2kg of Leymus chinensis every day. Feed once.
[0074] Schizochytrium powder: Schizochytrium powder was prepared according to the method in Example 1 above.
[0075] Addition method of Schizochytrium algae powder: according to the amount added in groups, add the test amount of each group to the concentrate and mix evenly.
[0076] (3) Feeding and manageme...
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