High-concentration liquid fermentation method of Bacillus coagulans
A technology of Bacillus coagulans and liquid fermentation, applied in microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of low bacterial content, high solid content, high processing cost, etc., and improve the spore rate. , The effect of reducing drying cost and production cost
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Embodiment 1
[0027] The present embodiment provides a high-concentration Bacillus coagulans liquid fermentation method, comprising the following steps:
[0028] (1) Bacillus coagulans was inserted into the selective medium, cultured in a constant temperature incubator at 40-45°C for 24 hours to obtain a single colony, and then a single colony was picked on the seed medium for seed culture and preservation.
[0029] Specifically, the selection medium is used for the separation and detection of Bacillus coagulans, and its components are as follows: glucose 0.5%, peptone 1%, yeast powder 0.5%, beef extract 0.5%, anhydrous calcium chloride 0.015%, sodium chloride 0.25% , 0.01% manganese sulfate monohydrate, 0.05% L-cysteine hydrochloride, the balance is water, the pH is adjusted to 5.0-5.5 with hydrochloric acid, then 1.5% agar powder is added, and it is prepared by sterilizing at 115°C for 30 minutes.
[0030] The composition of the seed medium is as follows: glucose 2%, peptone 2%, yeast e...
Embodiment 2
[0040] The high-concentration Bacillus coagulans liquid fermentation method provided by the present embodiment, its specific process is basically consistent with embodiment 1, and the difference is:
[0041] The composition of the selection medium is as follows: glucose 0.2%, peptone 2%, yeast powder 0.5%, beef extract 0.5%, calcium chloride anhydrous 0.1%, sodium chloride 0.1%, manganese sulfate monohydrate 0.03%, L-cysteine The hydrochloride is 0.05%, the balance is water, the pH is adjusted to 5.0-5.5 with hydrochloric acid, then 1.5% agar powder is added, and it is prepared by sterilizing at 115°C for 30min.
[0042] The composition of the slant culture medium is as follows: Weigh 1 g of bran, add 90 mL of water and boil over low heat for 30 min to 1 h, filter the juice with gauze, dilute to 100 mL, add 1.5% agar powder, and sterilize at 115°C for 30 min.
[0043] The composition of the fermentation medium is as follows: glucose 0.5%, yeast extract 3%, corn steep liquor 1%...
Embodiment 3
[0047] The high-concentration Bacillus coagulans liquid fermentation method provided by the present embodiment, its specific process is basically consistent with embodiment 1, and the difference is:
[0048] The composition of the selection medium is as follows: glucose 1%, peptone 1.5%, yeast powder 1%, beef extract 0.2%, calcium chloride anhydrous 0.01%, sodium chloride 0.5%, manganese sulfate monohydrate 0.05%, L-cysteine 0.01% hydrochloride, the balance is water, use hydrochloric acid to adjust the pH to 5.0-5.5, then add 2% agar powder, and sterilize at 115°C for 30min to prepare.
[0049] The composition of the slant culture medium is as follows: Weigh 10 g of bran, add 90 mL of water and boil over low heat for 30 min to 1 h, filter the juice with gauze, dilute to 100 mL, add 1.5% agar powder, and sterilize at 115 °C for 30 min.
[0050] The composition of the fermentation medium is as follows: glucose 2%, yeast extract 1%, corn steep liquor 0.1%, potassium dihydrogen ph...
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