Composite fluorescent particle protected by superhydrophobic shell and preparation method thereof

Abstract

The invention discloses composite fluorescent particles with a superhydrophobic protective shell layer and a preparation method of the composite fluorescent particles. The method comprises the following steps: by using zinc nitrate hexahydrate and 2-methylimidazole as raw materials, introducing a rare earth complex as a fluorescent molecule to synthesize zeolite imidazate skeleton material-8 coated rare earth complex core fluorescent particles; in a mixed solvent of ethanol and water, by using zinc hexahydrate and imidazole-2-carboxaldehyde as raw materials, growing the hydrophobic zeolite imidazate skeleton material-90 shell layer in situ on the surfaces of the core fluorescent particles; and finally, performing fluorination modification on the surfaces of the core-shell structural composite fluorescent particles to obtain the composite fluorescent particles with the superhydrophobic protective shell layer capable of maintaining fluorescence stability. The composite fluorescent particles have good dispersibility and fluorescence stability, the average particle size is 60+ / -5 nm, the fluorescence lifetime can reach 1.8 ms, the quantum yield can reach 19.1%, and the composite fluorescent particles have huge application prospects in the fields of biological detection and fluorescence imaging.

Description

technical field [0001] The invention belongs to the technical field of synthesis of luminescent nanomaterials, and in particular relates to a composite fluorescent particle protected by a superhydrophobic shell and a preparation method thereof. Background technique [0002] With the in-depth research on the pathological process of tumors and its subsidiary features, many characteristic markers such as specific proteins and small peptide molecules have been discovered by researchers. To a certain extent, these characteristic markers can be used as a detection signal source to reflect A cell or tissue in the body that may become cancerous. Due to the low cure rate and high cost of current cancer treatment, the development of early tumor diagnosis technology to curb the occurrence of tumor has become one of the hot research issues today. [0003] At present, the simple, efficient, accurate and reliable suspension array diagnostic technology is one of the latest technologies fo...

AI Technical Summary

Problems solved by technology

First, because its porous structure is in an ionic and aqueous environment, it cannot limit the penetration of ions and water into the MOF to quench the fluorescence of fluorescent dyes; second, under the strong coordination of foreign anions to metal ions in MOF materials , the topological structure of MOF materials is prone to amorphization, which affects the spatial structure of fluorescent molecules inside the particle, causing its fluorescence intensity to weaken or even disappear.

[0005] It can be seen that there are still problems in the existing preparation technology: due to the strong coordination of various ions in the environment to be tested, the complex fluorescent particles based on MOF materials as carriers often lead to the coordination of rare earth complexes due to unstable structures. The structure changes, so that the fluorescence stability cannot be guaranteed, and it is difficult to apply to the field of fluorescent labeling

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