Weissella fusion MP4 strain with antibacterial effect and application thereof
A Weissella and bacteriostatic technology, applied in the field of fusion Weissella MP4 strains, can solve the problems affecting the sustainable and healthy development of agriculture, excessive pesticide residues, environmental pollution, etc., and achieve the effect of good market application prospects.
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[0025] 1. Isolation and screening of strains
[0026] On June 15, 2017, 30 grape root samples were collected in Daze Mountain, Qingdao. The samples were transferred to a sterilized mortar for grinding, diluted with sterile water to 10ml, and then diluted to 10ml with sterile water. -3 -10 -4 Take 200ul of PDA-coated plates (200g / l potato, 20g / l glucose, 20g / l agar, natural pH), and culture them statically at 26°C.
[0027] Pick a single colony and inoculate a single bacterial colony in MRS (peptone 10.0g / l, beef extract 10.0g / l, yeast extract 5.0g / l, glucose 20.0g / l, triammonium citrate 2.0g / l , sodium acetate 5.0g / l, MgSO 4 0.1g / l, MnSO 4 0.05g / l, K 2 HPO 4 2.0g / l, pH 6.5) in liquid culture medium at 26°C for 48h, then centrifuged at 12,000rpm at 4°C, and the supernatant was filtered through a filter with a diameter of 0.22um for later use.
[0028] The filtrate was added to the MRS medium according to different proportions, and the conidia of Botrytis cinerea and Fus...
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