A bacteriostatic fusion Weissella mp4 bacterial strain and its application
A technology of Weissella and bacteriostasis, which is applied in the field of fusion of Weissella MP4 strains, can solve problems affecting sustainable and healthy agricultural development, environmental pollution, and reduced control efficiency, and achieve good market application prospects
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[0025] 1. Isolation and screening of strains
[0026] On June 15, 2017, 30 grape root samples were collected in Daze Mountain, Qingdao. The samples were transferred to a sterilized mortar for grinding, diluted with sterile water to 10ml, and then diluted to 10ml with sterile water. -3 -10 -4 Take 200ul of PDA-coated plates (200g / l potato, 20g / l glucose, 20g / l agar, natural pH), and culture them statically at 26°C.
[0027] Pick a single colony and inoculate a single bacterial colony in MRS (peptone 10.0g / l, beef extract 10.0g / l, yeast extract 5.0g / l, glucose 20.0g / l, triammonium citrate 2.0g / l , sodium acetate 5.0g / l, MgSO 4 0.1g / l, MnSO 4 0.05g / l, K 2 HPO 4 2.0g / l, pH 6.5) in liquid culture medium at 26°C for 48h, then centrifuged at 12,000rpm at 4°C, and the supernatant was filtered through a filter with a diameter of 0.22um for later use.
[0028] The filtrate was added to the MRS medium according to different proportions, and the conidia of Botrytis cinerea and Fus...
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